A method for improving xanthan gum production conversion rate

A conversion rate and xanthan gum technology, which is applied in the field of improving the conversion rate of xanthan gum fermentation production, can solve the problems of high metabolic residue, low fermentation rubber production rate, and low production conversion rate, so as to reduce fermentation metabolic residue and reduce production Cost, the effect of increasing corporate profits

Active Publication Date: 2017-07-04
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to increase the yield, the maximum amount of cornstarch will be put into the fermentation medium, but due to the limitations of the process and equipment, after the fermentation, there are still some starch hydrolyzates that have not been converted into the target product - xanthan gum, and its expression form is Low production conversion rate, low fermentation rubber yield and high metabolic residue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for improving the conversion rate of xanthan gum production, the method may further comprise the steps:

[0020] Step 1) Preparation of calcium salt solution: Dissolve calcium chloride in water to prepare a 15% (w / w) calcium chloride solution, maintain at 121°C for 30 minutes, then cool down to 35°C;

[0021] Step 2) Prepare sodium citrate solution: Dissolve sodium citrate in water to prepare a 2% (w / w) sodium citrate solution, maintain at 121°C for 30 minutes, and cool down to 35°C;

[0022] Step 3) Stirring: When fermenting for 40 hours, press 1L / m into the fermentation broth 3 Add the calcium chloride solution in step 1), stir at 500 rpm for 3 minutes; then add 1L / m to the fermentation broth 3 Add the sodium citrate solution in step 2), and stir for 3 minutes at 500 rpm;

[0023] Step 4) Enzymolysis: adjust the pH of the fermentation broth to 6.2, keep the temperature of the fermenter at 32°C, and press 480,000 U / m through feeding equipment 3 Add medium t...

Embodiment 2

[0031] A method for improving the conversion rate of xanthan gum production, the method may further comprise the steps:

[0032] Step 1) Preparation of calcium salt solution: Dissolve calcium nitrate in water to prepare a 20% (w / w) calcium nitrate solution, maintain at 121°C for 30 minutes, then cool down to 35°C;

[0033] Step 2) Prepare sodium citrate solution: Dissolve sodium citrate in water to prepare a 2% (w / w) sodium citrate solution, keep it at 121°C for 30 minutes, then cool down to 35°C;

[0034] Step 3) Stirring: When fermenting for 40 hours, press 1L / m into the fermentation liquid 3 Add the calcium nitrate solution in step 1), stir at 500 rpm for 3 minutes; then press 1L / m 3 Add the sodium citrate solution in step 2), and stir for 3 minutes at 500 rpm;

[0035] Step 4) Enzymolysis: adjust the pH of the fermentation broth to 6.2, keep the temperature of the fermenter at 35°C, and press 600,000 U / m through feeding equipment 3 Add medium temperature α-amylase to th...

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Abstract

The invention belongs to the technical field of production of xanthan gum and discloses a method for improving the conversion rate in production of xanthan gum. The method mainly comprises the steps of pre-processing a soluble calcium salt solution, supplementing a calcium salt solution and an enzyme solution, after the fermentation is completed, adding sodium hypochlorite, carrying out enzyme deactivation and inactivation, cooling, adding ethanol, extracting and drying to obtain the product. By the method, the utilization rate of a carbon source can be increased to as much as possible, the conversion rate in production of xanthan gum is improved, the fermentation metabolism residues are decreased and the method can bring better economic value.

Description

technical field [0001] The invention belongs to the technical field of xanthan gum production, and in particular relates to a method for improving the conversion rate of xanthan gum fermentation production. Background technique [0002] Xanthan gum, also known as xanthan gum, is a widely used microbial polysaccharide produced by Xanthomonas bacteria using carbohydrates as raw materials through fermentation. Xanthan gum is soluble in cold water and hot water, and forms a high viscosity after being fully hydrated. Solution, with efficient thickening effect, good temperature resistance, salt resistance, heat resistance and acid and alkali resistance. Xanthan gum has good water solubility and forms a high-viscosity solution after being fully hydrated. It is the current international product that integrates thickening, suspension, emulsification and stabilization. Bio-glue with the best performance; it can be used as emulsifier, stabilizer, gel thickener, wetting agent, film for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/06C12R1/64
Inventor 王均成张传森丁兆堂宋玉丽卢松陆秀香王伟徐彩凤
Owner 内蒙古阜丰生物科技有限公司
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