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Method for preparing enzyme capable of degrading ligno-cellulose

A technology of lignocellulose and culture medium, applied in the direction of hydrolytic enzymes, etc., can solve the problems of refractory natural lignocellulose, failure to meet production requirements, and low lignocellulose degradation efficiency, achieving less sugar consumption, low cost, and realization of The effect of energy regeneration

Inactive Publication Date: 2009-06-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under artificial culture conditions, it is difficult to directly decompose natural lignocellulose by relying on purely cultured microorganisms and enzymes.
At present, the degradation efficiency of lignocellulose in production is not high, and it is far from meeting the production demand. Therefore, it is necessary to find a method for efficiently degrading lignocellulose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation of the enzyme of degrading lignocellulose

[0021] The straws are treated as follows: stack rice straws into stacks, and then place them for more than 3 years at a temperature of -20°C to +20°C and a relative humidity of 20-30%, until the straws at the bottom of the stacks rot; take the rotten straws as samples to prepare enzymes.

[0022] 1. Preliminary cultivation: Take 5.0 g of the above-mentioned rotten straw, inoculate it into a 500 ml Erlenmeyer flask filled with 150 ml medium I and a 1 cm×6 cm filter paper strip, and cultivate under the following conditions: the temperature is 30° C., Aerobic, pH value is 7.0, shake at a speed of 150rpm / min, and the radius of rotation is 13mm; when the color of the filter paper strip turns yellow, that is, when the filter paper strip is degraded, the culture in the Erlenmeyer flask is 5% volume Inoculate into a 500ml Erlenmeyer flask filled with 150ml medium I and a 1cm×6cm filter paper strip, culti...

Embodiment 2

[0037] Embodiment 2, the preparation of the enzyme of degrading lignocellulose

[0038] The stalks are treated as follows: stack corn stalks into stacks, and then place them for more than 3 years at a temperature of -20°C to +20°C and a relative humidity of 20-30%, until the straws at the bottom of the stack rot; take the rotten straw as samples to prepare enzymes.

[0039] 1. Preliminary cultivation: Take 5.0 g of the above-mentioned rotten stalks, inoculate it into a 500 ml Erlenmeyer flask containing 150 ml medium I and a 1 cm×6 cm filter paper strip, and cultivate under the following conditions: the temperature is 28° C., Aerobic, with a pH value of 6.8, shaking at a speed of 120rpm / min, and a radius of rotation of 13mm; when the color of the filter paper strip turns yellow, that is, when the filter paper strip is degraded, put all the cultures in the Erlenmeyer flask with 5% Inoculate by volume into a 500ml Erlenmeyer flask containing 150ml medium I and a 1cm×6cm filter ...

Embodiment 3

[0051] Embodiment 3, the preparation of the enzyme of degrading lignocellulose

[0052] The stalks are treated as follows: the soybean stalks are stacked into stacks, and then placed at a temperature of -20°C to +20°C and a relative humidity of 20-30% for more than 3 years until the straws at the bottom of the stack rot; the rotten straws are used as samples to prepare enzymes.

[0053] 1. Get 5.0 g of the above rotten stalks, inoculate it into a 500 ml Erlenmeyer flask filled with 150 ml medium I and a 1 cm × 6 cm filter paper strip, and cultivate under the following conditions: temperature is 32 ° C, aerobic, The pH value is 7.2, shake at a speed of 180rpm / min, and the radius of rotation is 13mm; when the color of the filter paper strip turns yellow, that is, when the filter paper strip is degraded, inoculate all the cultures in the Erlenmeyer flask at a volume ratio of 5% Transfer to a 500ml Erlenmeyer flask filled with 150ml medium I and a 1cm×6cm filter paper strip, cult...

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Abstract

The invention discloses a method for preparing an enzyme for degrading lignocellulose. The method comprises the following steps: using a culture medium I to culture treated straws and obtaining culture I; and obtaining the enzyme for degrading the lignocellulose from the culture I. The compositions of the culture medium I comprise the following: each liter of the culture medium I comprises 4 to 6 grams of peptone, 0.8 to 1.2 grams of yeast extract, 4 to 6 grams of NaCl, 0.8 to 1.2 grams of K2HPO4, 0.30 to 0.40 gram of MgSO47H2O, 2.5 to 3.5 grams of CaCO3 and 0.1 to 0.2 gram of the straws, and water is taken as a solvent. As proved by experiments, the enzyme prepared by the method has high activity, high purity, high efficiency of decomposing the natural lignocellulose, small sugar consumption, and small number of byproducts, can effectively convert the cellulose into sugar, and realize energy regeneration. Simultaneously, the method has the advantages of low cost and simple operation. Therefore, the method has important application value in the aspect of degrading the cellulose.

Description

technical field [0001] The invention relates to a method for preparing an enzyme for degrading lignocellulose. Background technique [0002] Composed of lignin, hemicellulose and cellulose, lignocellulose is a potentially renewable resource that can be degraded to produce disaccharides or monosaccharides. Monosaccharide itself is a kind of energy substance, which can also be processed into other energy substances such as alcohol. Lignocellulose is the most abundant natural organic matter in the world, accounting for more than 50% of the carbon content in the plant kingdom, and its sources are extensive, such as cotton, wood, hemp, wheat straw, straw, bagasse, etc. are rich sources of cellulose. In today's energy shortage situation, degrading lignin can not only produce energy substances, but also turn waste into treasure, which is a double win. [0003] In recent years, lignocellulose degradation has become a research hotspot. Methods for degrading lignocellulose include ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24
Inventor 崔宗均王小芬郭鹏王小娟王慧曹燕篆
Owner CHINA AGRI UNIV
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