Method for increasing yield of 5-hydroxytryptophan

Abstract

The invention provides a method for increasing the yield of 5-hydroxytryptophan. In the acid production stage of the middle fermentation stage, the ventilation quantity is reduced, the dissolved oxygen is reduced and citric acid is fed, so that sufficient reducing power in the BH4 synthesis process is ensured, the sugar supplementing rate is reduced after bottom sugar is consumed completely, and accumulation of tryptophan is reduced; according to the method, the yield of 5-HTP is increased and the intermediate product tryptophan is reduced by controlling dissolved oxygen to a suboptimal dose of cells, feeding citric acid to inhibit an EMP pathway, enhancing an HMP pathway and reducing the glucose feeding rate in the fermentation process, the intermediate product tryptophan is reduced, the problems that tetrahydropterin is oxidized and consumed in the biological fermentation process, the reducing power in the synthesis process is insufficient, and the yield of 5-HTP is affected due to excessive accumulation of tryptophan are solved.

Description

technical field [0001] The invention relates to the field of amino acid production by fermentation, in particular to a method for increasing the yield of 5-hydroxytryptophan. Background technique [0002] Depression is a common mental disorder that threatens millions of people around the world. Insufficiency of the neurotransmitter serotonin in the central nervous system (CNS) is thought to be an important physiological factor in depression. [0003] 5-Hydroxytryptophan (5-HTP) is the direct biosynthetic precursor of serotonin in humans and animals. It has been shown to be clinically effective in the treatment of depression with relatively few side effects. 5-Hydroxytryptophan (5-HTP) is a natural amino acid that does not participate in protein synthesis. It is produced by replacing the 5' hydrogen atom on the benzene ring of tryptophan with a hydroxyl group. The chemical name is 5-hydroxy-3- Indolyl-alpha-alanine. In mammals, 5-HTP is the precursor of the neurotransmitt...

AI Technical Summary

Problems solved by technology

However, in general, the chemical synthesis reaction system is relatively complicated and the steps are cumbersome. Therefore, microbial synthesis is a 5-HTP production method with great potential due to its low cost, simple operation, green environmental protection, and mild reaction.

However, the current microbial method still has the problems of catalyzed enzymes, whose enzyme activity is unstable, and expensive cofactors need to be added from an external source, resulting in high cost and low catalytic efficiency.

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