Production method for efficiently secreting and expressing porcine epidemic diarrhea virus S1 protein

A technology for porcine epidemic diarrhea and secretion expression, applied in biochemical equipment and methods, viruses, viral peptides, etc., can solve problems such as losses and unsatisfactory effects in the pig industry, save labor and material costs, cost-effective, and improve stability. sexual effect
CN113336833AInactive Publication Date: 2021-09-03GUANGZHOU BONIZZI BIOTECH CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU BONIZZI BIOTECH CO LTD
Publication Date
2021-09-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a production method for efficiently secreting and expressing porcine epidemic diarrhea virus S1 protein. According to the invention, amplifying and expressing porcine epidemic diarrhea virus S1 protein through a poly-fiber paper carrier by using a cell bioreactor, culturing for 2-3 days by using a DMEM (Dulbecco Modified Eagle Medium) containing 10% fetal calf serum in a cell culture stage, discarding all the culture medium, washing with PBS (Phosphate Buffer Solution) for 1-2 times to remove the serum, then completely replacing the culture medium with a serum-free culture medium and starting to produce and express the porcine epidemic diarrhea virus S1 protein; collecting part of the culture medium every 3-5 days, adding serum-free culture medium again, and supplementing glucose according to the sugar consumption of cells every day to keep the concentration of the glucose is kept at 4 g / L. By adopting the method provided by the invention, the expression level of cells is effectively improved, the manufacturing cost of vaccines is reduced, and a foundation is laid for realizing large-scale production of vaccines.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a production method for efficiently secreting and expressing porcine epidemic diarrhea virus S1 protein. Background technique

[0002] Porcine epidemic diarrhea (Porcine epidemic diarrhea, PED) caused by porcine epidemic diarrhea virus (Porcine epidemic diarrhea virus, PEDV) is characterized by vomiting of pigs, watery diarrhea, dehydration and high mortality of suckling piglets. Sexual intestinal diseases. In the 1970s, the disease was mainly prevalent in major pig producing countries in Europe; in 1982, it was first discovered in Japan in Asia and spread rapidly to neighboring countries. In 1984, Xuanhua and others confirmed the existence of the disease in our country through fluorescent antibody test and serum neutralization test, and it is widely prevalent in our country at present. The epidemic of PEDV affects pig herds of all ages in many provinces of China, with ...

Claims

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