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Spreading cultivation method for saccharomycetes, application of spreading cultivation method and method for fermenting ethanol

A yeast and yeast technology, which is applied in the field of ethanol fermentation and yeast expansion, can solve the problems of low sugar utilization rate, high equipment investment intensity, and bacteria pollution, etc., achieve high activity, improve sugar alcohol conversion rate and woodiness Sugar consumption rate and the effect of avoiding bacterial contamination

Active Publication Date: 2017-06-27
COFCO GROUP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are following defects in the aforementioned expansion method: on the one hand, it is easy to cause miscellaneous bacteria pollution in the expansion cultivation, and the equipment investment intensity is large; on the other hand, it is difficult to effectively control the amount of yeast obtained, which often leads to overcultivation (expansion) The number of yeast in the nutrient solution cultivated is at least 300 million / ml), causing a considerable amount of sugars to be used for the metabolism of the yeast body itself during fermentation, resulting in lower utilization of sugars for ethanol fermentation; third aspect, The vigor (mainly reflected in the conversion rate of sugar alcohol and the consumption rate of xylose during fermentation) of the yeast thus obtained is relatively low

Method used

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preparation example Construction

[0018] In the present invention, the method for preparing the above-mentioned liquefied mash is not particularly limited, and can be various methods commonly used in the art. Preferably, the preparation method of the liquefied mash includes: crushing the starch raw material, followed by hydrothermal treatment and solidification. Liquid separation treatment, wherein the conditions of the hydrothermal treatment include: the temperature is 75-85° C., the pH value is 4.5-5, and the time is 3-4 hours. Those skilled in the art should understand that the hydrothermal treatment can gelatinize-liquefy the starch and destroy cells to form a uniform liquefied mash.

[0019] In the present invention, preferably, the starch raw material is corn and / or cassava.

[0020] In the present invention, preferably, the preparation method of liquefied mash further includes: adding liquefaction enzyme to the product obtained from solid-liquid separation treatment for liquefaction treatment, wherein, ...

Embodiment 1

[0041] (1) Preparation of corn liquefaction mash: use 2.5kg of corn as raw material, crush it, then hydrothermally treat it at 80°C and pH value of 4.8 for 3 hours, and then filter to obtain 10L corn liquefaction mash filtrate, then use the crushed corn Based on the weight of the raw material (2.5kg), 0.2 wt‰ of liquefaction enzyme was added to the corn liquefaction mash filtrate for liquefaction treatment. The conditions of liquefaction treatment included: temperature 105°C, pH value 6.5, time 0.5h. Finally, taking the volume of the product obtained by the liquefaction treatment as a benchmark, add 0.5g / L potassium dihydrogen phosphate and 0.5g / L urea to the product obtained by the liquefaction treatment, and mix uniformly to obtain 10L corn liquefaction mash. The DE value of the liquefied mash was 75.

[0042] (2) Under aseptic conditions, first add 3L of corn liquefaction mash to the 10L seed tank, then inoculate the activated yeast liquid into the seed tank with an inoculu...

Embodiment 2

[0048] (1) Preparation of corn liquefaction mash: use 2.5kg of corn as raw material, pulverize, then hydrothermally treat at 85°C and pH value of 4.5 for 3.5h, then filter to obtain 10L corn liquefaction mash filtrate, and then use the pulverized Based on the weight of corn raw material (2.5kg), add 0.1% by weight of liquefying enzyme to the corn liquefaction mash filtrate for liquefaction treatment. The conditions of liquefaction treatment include: temperature is 95°C, pH value is 6.5, and time is 1.5h. Finally, based on the volume of the product obtained by liquefaction treatment, add 0.8g / L potassium dihydrogen phosphate and 0.3g / L ammonium bicarbonate to the product obtained by liquefaction treatment, and mix uniformly to obtain 10L corn liquefaction mash. , the DE value of corn liquefaction mash is 70.

[0049] (2) Under aseptic conditions, first add 2.4L corn liquefaction mash to the 10L seed tank, then inoculate the activated yeast liquid into the seed tank with an inoc...

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Abstract

The invention relates to the technical fields of spreading cultivation of microorganism strains and fermentation of ethanol and discloses a spreading cultivation method for saccharomycetes, application of the spreading cultivation method and a method for fermenting ethanol. The spreading cultivation method comprises the step of with liquefied mash as a raw material, sequentially carrying out intermittent spreading cultivation and continuous spreading cultivation on activated saccharomycetes under an aseptic condition. The implementation manner of the continuous spreading cultivation comprises the step of simultaneously introducing the liquefied mash and discharging a culture solution in the same volume flow rate after the intermittent spreading cultivation is finished, wherein the volume flow rate is (0.1-0.6)V / h, and V is the volume of the culture solution when the intermittent spreading cultivation is finished. By utilizing the spreading cultivation method, the number of the saccharomycetes can be effectively controlled, the activities of the obtained saccharomycetes are relatively high, and the sugar alcohol conversion rate and wood sugar consumption rate in a fermentation process can be obviously increased.

Description

technical field [0001] The invention relates to the technical field of microbial strain expansion and ethanol fermentation, in particular to a method for expanding yeast and its application and a method for fermenting ethanol. Background technique [0002] Due to the non-renewability of fossil energy and the fact that resources are increasingly depleted, the development of liquid fuels using renewable materials (such as cellulose-containing biomass raw materials) has attracted more and more attention. After pretreatment and enzymatic hydrolysis of cellulose-containing raw materials to obtain available fermentable sugars, how to convert these sugars into liquid fuels (such as ethanol) is a key point in the entire conversion route. [0003] In the existing technology, available sugars, such as xylose, glucose, cellobiose, mannose, etc., are converted into ethanol mainly through microbial fermentation. Among them, such microorganisms include: Saccharomyces cerevisiae, Pachysol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/10C12R1/865
CPCC12N1/16C12P7/10Y02E50/10
Inventor 李凡沈乃东熊强张宏嘉唐堂
Owner COFCO GROUP
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