236results about How to "Small flux" patented technology

The invention provides a micro-fluidic device and a method for detecting circulating tumor cells on the basis of dimensions. The micro-fluidic device comprises a solution storage chamber, a micro-fluidic chip, a waste liquid collecting syringe and a power system which are connected in sequence, wherein the micro-fluidic chip is made of a glass substrate layer and a PDMS (Polydimethylsiloxane) chip layer in a laminated manner; the PDMS chip layer comprises an injection port, a block mass filtering area, a target sell screening area and a discharge port which are communicated in sequence; the block mass filtering area is formed by a column array; the target cell screening area comprises a plurality of main pipelines and side pipelines which are extended in a parallel manner at intervals and are communicated through filtering channels; the front ends of the main pipelines are opened; the filtering channels are formed in the rear ends of the main pipelines; the front ends of the side pipelines are sealed; the rear ends of the side pipelines are opened; the main pipelines and the side pipelines have a first height greater than the dimensions of the circulating tumor cells; the filtering channels have a second height smaller than the dimensions of the circulating tumor cells. The micro-fluidic device and the method for detecting the circulating tumor cells on the basis of the dimensions are good in sensitivity, simple to operate, low in cost, large in flux and short in time.

The invention discloses a compound reverse osmosis membrane with interpenetrating network desalting layer and a preparation method of the membrane. The desalting layer consists of a stereoscopic network which is formed by interpenetrating macromolecule nanogel and polyamide. The preparation method comprises the following steps of: pre-adding hydrophilic and / or oleophylic macromolecule nanogel with good dispersion into a solvent of solution A and / or B, preparing a water phase A solution containing m-phenylenediamine and / or p-phenylenediamine, preparing an organic phase B solution containing trimesoyl chloride, enabling the solution A and the solution B to fully contact on a porous support carrier for interface reaction, rinsing by using deionized water, carrying out moisturizing treatment by using glycerol, and drying. The compound reverse osmosis membrane disclosed by the invention has a sodium chloride removal rate of not less than 99% and a flux of not less than 25GFD (Geophysical Fluid Dynamics) under a pressure of 225psi; the microstructure and the charge property of the desalting layer can be regulated and controlled through the macromolecule nanogel so that the reverse osmosis membrane with high anti-pollution capability is prepared; and the compound reverse osmosis membrane can further be used for developing novel low-pollution reverse osmosis membranes.

The invention discloses a nucleic acid extraction and purification method based on nanometer magnetic beads, comprising the following steps: mixing a biological sample and a lysis buffer to make nanometer magnetic beads in the lysis buffer and nucleic acid DNA / RNA which moves into the lysis buffer form a magnetic bead-nucleic acid compound; transferring the compound under the action of a magnetic field to a washing buffer to wash off impurities on the magnetic bead-nucleic acid compound; and transferring the washed magnetic bead- nucleic acid compound under the action of the magnetic field to an elution buffer so as to elute and recover nucleic acid. The nanometer magnetic beads used in the invention have advantages of uniform size, smooth surface, large surface area ratio, high adsorption capacity of nucleic acid, fast magnetic response speed and rapid separation, and can be stored together with the lysis buffer at room temperature for a long time. The extracted nucleic acid DNA / RNA has high purity, is complete and can be directly used for follow-up detection. The method provided by the invention has shorter nucleic acid extraction time than a general magnetic bead method by the use of a nucleic acid extraction reagent, is more suitable for automation and is adopted to realize high-flux nucleic acid DNA / RNA extraction.

The invention relates to a membrane treatment process for oilfield reinjection water, which comprises the specific steps of: firstly introducing oil-containing wastewater into a settling tank for naturally settling to remove part of large suspended solid granules and waste oil; directly introducing the settled water into a ceramic membrane ultrafiltration device for internal circulating filtration, directly using the liquid penetrating through the membrane for reinjection, introducing the circulating liquid containing large amounts of suspended solid granules and waste oil which do not penetrate through the membrane into the settling tank for continuing settlement, mixing with inflow water and introducing into the ceramic membrane ultrafiltration device for filtration again; automatically discharging pollutants when the wastewater in a circulation tank reaches a certain concentration ratio; and automatically adjusting the membrane surface flow rate of an online detection device according to the inflow water quality conditions, so as to achieve the purpose of energy conservation. The membrane treatment process provided by the invention has the advantages of simple process, low investment cost, high filtering precision, no need for the introduction of any reagents during the treatment process, no secondary pollution, etc.

This invention relates to a method for prparing temperature-sensitive polyvinylidene flourine intelligent membrane. The method is: NIPA as grafted monomer, PVDF as macromelocular initiator, cuprous chloride as catalyst,4,4 demethyl 2,2 bipyridine as ligand, using atom transfer free radical process, preparation of temperature-sensitive polyvinylidene flourine intelligent membrane comprising: in reaction kettle, adding 7-13% PVDF(WT%) and solvent N-methyl-2-pyrrolidone,heating to 50 deg. C to make it solve completely; then adding grafted monomer NIPA, its mol ratio with cuprous chloride is 2000:1-2500:1,filling nitrogen gas 30mins. at stirring, and separately adding 0.20-025g ligand 4,4-dimethyl 2,2-bipyridine and 0.04-0.06g catalyst cupous chloride, stirring and heating to 80-120deg.C,constant reaction 19-25 hrs; then washing reaction product using pure water to obtain light brown solid, filtering and 80 C degree drying to obtain finish product.

The invention relates to a hydrophilic polyolefin-based composite nanofiltration membrane. The membrane is characterized in that a polyolefin microporous membrane is immersed in a hydrophilic agent toobtain a hydrophilically modified polyolefin membrane; and a polyamide selective skin layer is formed by interfacial polymerization of polyamine water-phase monomers and poly acyl chloride oil-phasemonomers on the hydrophilically modified polyolefin membrane. The membrane disclosed by the invention has the positive effects that compared with a traditional ultrafiltration base, the adopted polyolefin film base is higher in water flux, long in flux retention time and stable in performance; a hydrophilic modification process of the polyolefin film is simple and easy to operate and strong in controllability and the modified membrane can be permanently hydrophilic, so that a capability of continuous large-scale industrial production is provided; and the composite nanofiltration membrane prepared by adopting the polyolefin base is high in water flux, low in NaCl intercepting rate and high in Na2SO4 intercepting rate and has a good charge separating ability.

An imaging system including a source (310) having a focal spot (406) that emits a radiation beam that traverses an examination region, a radiation sensitive detector array (316) having a plurality of pixels that detects radiation traversing the examination region and generates projection data indicative of the detected radiation, and a filter (314), disposed between the source and the examination region, that filters peripheral regions of the emitted radiation, wherein the filter includes two separate and moveable regions (402), each region having a substantially same thickness and constant homogeneity.

The invention belongs to the technical field of composite reverse osmosis membranes and particularly relates to a preparation method of a modified pollution-resistant hybridized reverse osmosis membrane. According to the preparation method, by introducing a combined inorganic nano-particle of a graphene oxide nano-particle, nano-Ag and nano-SiO2 an dissolving the combined inorganic nano-particle by virtue of a compound organic solvent, the result shows that an inorganic nano-material has relatively good dispersity in a polymer film matrix and further has relatively good consistency between twophases of the inorganic nano-particle and a polymer. A polyamide functional layer is hybridized and is grafted with hydrophilous an NH2-PEG-OH molecular chain through chemical reaction, so that the hydrophily of a membrane is improved, and the prepared composite membrane has relatively excellent antibacterial property, pollution resistance and thermal stability and further has huge prospects in industrial water treatment.

This invention relates to pressure difference controlled self-adapting throttle expansion valve, which comprises pressure difference inductance structure and elastic elements in outer shell and elastic wave pattern tube, wherein the elastic element both ends are separately connected to the elastic wave pattern tube sealed end outside and outer shell inner top end and the elastic wave tube bottom is connected to the outer shell bottom end. The elastic wave pattern tube and the outer shell form chamber A. The flow adjusting structure comprises drive bar connected to the elastic wave tube sealed end inner top, valve needle and valve with one hole for the needle move. Its sealed upper end is connected to the drive down end.

The invention discloses a method for synthesizing zeolite socony mobil-5 (ZSM-5) zeolite membrane by dehydration by acetic acid, which comprises: directly introducing an ultrathin crystal seed layer onto a macroporous carrier by a two-step crystal process, regulating the Si to Al ratio of a crystallization mother solution by using F<-> as an inorganic structural guiding agent, and preparing a 5 to 6 micrometer thick ZSM-5 zeolite membrane, particularly a ZSM-5 zeolite membrane in which the Si to Al ratio is 10, by using a secondary growth process; therefore, the limit that the membrane can only be formed under a condition that the Si to Al ratio of the crystallization mother solution is more than 20 if an organic template agent is not used is break through, the hydrophilic property of themembrane is improved greatly, and the generation of microstructures which are not acid resistant with aluminum enriched surface and inter-crystal gap is avoided effectively. The membrane thus has high acetic acid dehydration and separation selectively and high acid resistance, the low-flux, low-selectivity and low-acid-resistance technical bottlenecks that zeolite membrane organic acid dehydration faces are broken through, and a firm foundation is laid for the industrial use of the zeolite membrane.

The invention provides a processing method of high-purity aloe acetylated polysaccharide, comprising the steps of: peeling and crushing the aloe, filtering to obtain the aloe juice, adding the pectinase in the aloe juice according to the additive amount of 50-70 ml / ton of aloe juice, adding the active carbon in the aloe juice according to 0.1-0.3 of the mass of the aloe juice, then stirring for 20-40 min for enzymolysis and decolorization, and standing until the aloe juice is clear; thereafter, filtering by the microfiltration membrane with filtration aperture of 10 mu m, the filtration membrane with filtration aperture of 0.2 mu m and the ultrafiltration membrane with filtration aperture of 0.001 mu m, concentrating by the reverse osmosis membrane and drying to obtain the aloe powder, the mass content of the aloe acetylated polysaccharide reaches 15%. The invention realizes continuous separation at the normal temperature and enables various functional compositions in the aloe to be fully separated without adding any assistant, thereby the purity of the aloe acetylated polysaccharide is guaranteed and the extraction and separation costs are reduced.

The invention discloses a SNP loci combination for growth performance of meat sheep and an application thereof, and relates to the technical field of gene breeding. According to the invention, 2036 SNP loci are provided, and physical position information of the 2036 SNP loci is determined based on comparison of sheep v4.0 genome sequences. The SNP loci are SNP0001-SNP2036. Genetic evaluation is performed on sheep individuals based on the 2036 SNP loci, thereby achieving individual selection on growth traits difficult to measure in the early stage, shortening generation interval, accelerating breeding process and thus saving substantial breeding cost.

The invention relates to a high efficient complex enzyme sewage treatment process which is carried out according to the following steps: (1) massive grains such as suspended substances in sewage are removed through a grid, and then the sewage enters a regulating reservoir; (2) the sewage in the regulating reservoir enters a high efficient complex enzyme catalyzing-membrane bioreactor device; (3) the sewage passes through a membrane module of the high efficient complex enzyme catalyzing-membrane bioreactor device, and clear water penetrates through the membrane module and flows out; (4) a concentrated solution which cannot penetrates through the membrane module enters a biological processing part again so as to be further catalyzed and deteriorated; and (5) the steps are repeated till the processed water reaches the reuse water discharging standard. The invention also relates to a high efficient complex enzyme sewage treatment device. The process and the device makes up the detects of the current MBR process, such as membrane pollution, high expense, and the like, and can rapidly deteriorate pollutants, rapidly and high efficiently purify the sewage, achieve good treatment effect and greatly lower the running cost for sewage treatment.

The invention relates to a spent lubrication oil regeneration device, which comprises a first heater, a precipitator, a second heater, a solid-liquid separator, an electric dehydrator, the ultrasonic membrane separator, a third heater, a catalytic cracking reactor and a fractionating tower, which are connected in sequence. An ultrasonic part is disposed at the front of a filter membrane inside the ultrasonic membrane separator, the filter membrane in the ultrasonic membrane separator is a hollow fiber membrane resistant to 80-100 DEG C and resistant to oil corrosion, and average pore size of the filter membrane is 0.1-0.5 micrometers. Spent lubrication oil is separated by the ultrasonic membrane separator to obtain light oil and heavy oil, the light oil is discharged from an oil outlet and collected for use, the heavy oil is discharged from a concentrated oil outlet into the catalytic cracking reactor to generate light oil, base lubrication oil and non-hydrocarbon gases after reaction, and the light oil, the base lubrication oil and the non-hydrocarbon gases are separated and used respectively. The spent lubrication oil regeneration device is applicable to the field of recycle of spent lubrication oil.

The invention relates to an on-line back washing process system of a porous ceramic film. A cleaning liquid is extruded into a ceramic film assembly by adopting air pressure or a submersible pump, and a cleaning liquid discharge switch is arranged, and the pressure required by back washing can be lowered; a switch and a soundproofing device are installed above a cleaning tank, and the noise pollution caused by air in a cleaning liquid storage tank is solved; the opening and closing time and the pressure of an air pump are regulated through a time relay, thus the liquid in the cleaning liquid storage tank is always maintained to be 1 / 3 of the volume of the cleaning liquid storage tank; and through installing the cleaning liquid discharge switch at a material discharge end of a filtering device of the porous ceramic film, most of the cleaning liquid can be recycled, thus the cleaning liquid can be prevented from circulating and entering an original liquid storage tank, and the secondary pollution to the film is prevented. The system not only can improve the film flux recovery speed, the film flux recovery rate can reach 95% above, and water is saved and electricity is saved. The on-line back washing process system of the porous ceramic film provided by the invention is mainly applied to the back washing process of the application of the porous ceramic film in the liquid filtering process, can effectively recover the film flux, and is beneficial to popularization and application..

The invention relates to a microfluidic chip capable of being used for enzyme-linked immunoassay (ELISA) which is low in cost and easy to prepare. The microfluidic chip comprises a microfluidic layer, a control layer and a connecting layer for connecting the microfluidic layer with the control layer, wherein a microfluidic passage in the microfluidic layer and a control passage in the control layer have at least one overlap point; the connecting layer at the position of the overlap point is made of an elastomer material; when pressure in the control passage is increased to a certain value, the elastomer connecting layer at the position of the at least one overlap point will expand towards the microfluidic layer and is in contact with the inner surface of the microfluidic passage to form a contact surface of a certain size; the width of the contact surface formed on at least one part of overlap points is less than that of the microfluidic passage at the overlap point, so that the microfluidic passage is in a communication state; and when the pressure in the control passage is restored, the elastomer connecting layer at the position of the overlap point is restored to the original state. Through the structure, an ELISA reaction region can be conveniently formed in the chip, so that the ELISA is quickly performed.

The invention discloses an advanced treatment and reuse method of a pre-coated membrane of tail water of a sewage plant. The method comprises the following steps: firstly, feeding tail water into an adjusting pool so as to be homogenized, and then passing through a sand filter; passing through an ultrafiltration membrane by a solution containing a pre-coated membrane material and sand filtered effluent and effectively forming a dynamic membrane on the surface of the ultrafiltration membrane, wherein concentrated water of the ultrafiltration membrane returns to the adjusting pool while the effluent enters into a middle water tank; backwashing the ultrafiltration membrane through a pump by part of water in the middle water tank periodically, wherein the pre-coated membrane automatically falls and the sediment of the coating material is recycled to a pre-coated membrane material dosing device repeatedly, so that the operating cost is lowered; entering into reverse osmosis membrane treatment by water in the middle water tank, fresh reverse osmosis membrane is used as industrial water in the park to be returned, and the concentrated water is treated by an electrochemical reactor, and the effluent reaches the standard to be discharged. By adopting the pre-coated membrane method, the ultrafiltration backwashing frequency is low, and the water yield of the ultrafiltration membrane is improved. Loose filter cake layers with great holes can be formed to effectively relieve the rate of descent of membrane flux, improve the anti-pollution capacity of the membrane and improve the removal effect of the membrane on the water quality index at the same time.

Belonging to the technical field of reverse osmosis membranes, the invention particularly relates to a high-flux anti-pollution reverse osmosis membrane and a preparation method thereof. The reverse osmosis membrane sequentially comprises a polysulfone based membrane layer, a polyamide desalination layer and a polyvinyl alcohol protective layer from the bottom up. The polyvinyl alcohol protectivelayer comprises the following components by mass: 1 part of polyvinyl alcohol, 0.02-0.1 part of nano titanium dioxide modified clay minerals and 0.2 part of 3-aminopropyl silane triol. The invention also provides a preparation method of the high-flux anti-pollution reverse osmosis membrane. The method provided by the invention solves the problem of water flux reduction after coating of the polyvinyl alcohol protective layer, at the same time enhances the anti-pollution performance of the reverse osmosis membrane, and the reverse osmosis membrane can still keep excellent water flux and desalination rate after long-term flush pollution of surface water.

The invention discloses a polyarylene sulfide sulfone compound separating film and a preparation method thereof. The polyarylene sulfide sulfone compound separating film comprises a polyarylene sulfide sulfone base film and a selective separating functional layer, wherein the selective separating functional layer is prepared from any one of fatty polyamide, aromatic polyamide, methyl-silicone andcellulose acetate through a coating method or an interfacial polycondencation method. According to the invention, the selective separating functional layer is added to the polyarylene sulfide sulfonebase film and the defects on the surface of the polyarylene sulfide sulfone base film are covered by the compact selective separating functional layer, so that the surface of the polyarylene sulfide sulfone compound separating film has a uniformly distributed pore structure, the porosity is increased and the pore size distribution is uniform; the interception capacity thereof is obviously promotedafter the selective separating functional layer is added; when the selective separating functional layer with hydrophilic group is adopted, hydrophilic modification for the polyarylene sulfide sulfone is not required.

The invention provides a method for adjusting the operation condition to control or mitigate pollution of the membrane bioreactor, which relates to a method for controlling or mitigating the membrane pollution, aiming at solving the problems that using the aerobic particle sludge inoculation to control the membrane pollution dose not conform to the initial purpose of inventing the membrane bioreactor which influences the utilization efficiency of the membrane bioreactor. In the aerobic membrane bioreactor, the invention ensures that the dissolved oxygen concentration is 3-4mg / L; the sludge concentration 8000-10000mg / L; the hydraulic retention time is 7-9 hours; the sludge retention time is 140-160d. Microorganism is added to the anaerobic membrane bioreactor, in which the sludge temperature is controlled to range from 20 to 30 DEG C and the pH value is controlled at 6.3-7.7. The invention controls from reducing the microorganism species of the secretion membrane pollutants. The reduction of the membrane flux of 100 days is 50 percent of the contraction and the times of membrane-clearing are reduced by 70 percent. Compared with the contraction, the invention saves the operation cost more than 70 percent, thus improving the sewage quantity of 150 Ld<-1>.

The invention relates to an antibody chip reagent kit for detecting a hepatoma marker. The antibody chip reagent kit comprises a glass slide, a soft silicone rubber pad, a hard frame and reaction grooves formed by U-shaped frame clamps, wherein the hard frame is divided into 2*8 or 4*16 sub-boxes so as to form the 16-hole or 64-hole frame; the size of the soft silicone rubber pad corresponds to those of the hard frame and the standard glass slide; each sub-box of the hard frame forms the corresponding small reaction groove; the standard glass slide in each of the small reaction grooves is bonded with specific antibodies with corresponding concentrations; the specific antibodies are antibodies for the following 8 selected tumor markers: AFP, AFP-L3, DCP, AFU, GP73, SCCA, CK19 and VEGF. The antibody chip reagent kit adopts the antibody chip technology, so that 8 types of hepatoma markers can be detected at a time, the defect that the conventional single marker detection method is big in sample usage amount and long in detection time is avoided, and the advantages of high detection speed, less sample consumption, high sensitivity, good stability and the like are achieved.

The invention discloses a method for synthesizing 2-(4-fluorophenyl) thiophene. The method comprises the following steps: 1) preparing 4-fluorophenylboronic acid: separately preserving heat for a 4-fluorophenylmagnesium bromide solution which is taken as a material 1 and a boric acid ester solution which is taken as a material 2 to the reaction temperature, carrying out mixing reaction, and carrying quenching and post-treatment to obtain 4-fluorophenylboronic acid; and 2) preparing 2-(4-fluorophenyl) thiophene): adding the 4-fluorophenylboronic acid, 2-bromo thiophene and a Pd catalyst into an organic solvent to dissolve to obtain a material 3, separately preserving the heat for the material 3 and an inorganic alkali aqueous solution which is taken as a material 4 to the reaction temperature, carrying out mixing reaction, and carrying out post-treatment to obtain 2-(4-fluorophenyl) thiophene. The method is simple in operation process, is short in production period, is gentle in condition, is fewer in side reactions and is high in yield; and the obtained product is high in purity.

The invention belongs to the technical field of nucleus technology application, and relates to an on-line analysis device of pulsed neutron coal element content and proximate analysis indexes. The on-line analysis device comprises a radiation protector, a coal conveying channel and a detection device; the coal conveying channel and the detection device are arranged in the radiation protector; the detection device is positioned below the coal conveying channel; the detection device comprises a neutron detector, a pulsed D-T neutron generator and a BGO (bismuth germanium oxide) detector and is characterized in that a lead plate a, a polyethylene plate and a lead plate b are sequentially arranged between the pulsed D-T neutron generator and the BGO detector; and the BGO detector is positioned in a BGO detector constant temperature control system, with a temperature range kept at -4.5 DEG C to -5.5 DEG C. According to the invention, the on-line analysis device is not influenced by parameters such as changes of coal type, speeds of a belt for conveying coal, thickness changes of coal seams, particle sizes of coal and the like, so that the precision for measuring parameters of coal property is high.

The invention discloses an electro-catalytic membrane wastewater treatment system. The system comprises an organic wastewater pool, a mixed valve, a direct current regulated power supply, an electro-catalytic membrane tube, an auxiliary electrode and a penetrating fluid tank, wherein the electro-catalytic membrane tube comprises a ceramic membrane tube substrate which is capable of achieving carbonization conduction by thermal decomposition and chemical vapor deposition of propylene carbon source gas; a catalyst for realizing electro-catalytic oxidation of wastewater is supported on the ceramic membrane tube substrate; the electro-catalytic membrane tube is installed in the penetrating fluid tank; the auxiliary electrode surrounds outside the electro-catalytic membrane tube and an interval is maintained between the auxiliary electrode and the electro-catalytic membrane tube; the water outlet end of the organic wastewater pool is connected with the water inlet end of the mixed valve through a metering pump; the mixed valve is connected with the electro-catalytic membrane tube through a water inlet pipe; the positive electrode and negative electrode of the direct current regulated power supply are respectively connected to the electro-catalytic membrane tube and the auxiliary electrode through wires; and the water outlet end of the electro-catalytic membrane tube is connected with the mixed valve, and a control valve is arranged between the electro-catalytic membrane tube and the mixed valve. The treatment of difficultly-degradable organic wastewater can be realized through the system.

A submerged membrane bioreactor with non-fabric filter is composed of a tank containing microbial mixture liquid and a fitler module submerged in the said mixture liquid. The filter module has a penetrable surface for passing the mixture liquid through it, and is composed of a porous supporting material with the said penetrable surface and a second surface, and a non-fabric membrane with a thickness between 0.1-2 mm and pores between 0.2-100 microns covering on the second surface.

The invention provides a separating membrane pollution controlling and cleaning method, comprising: adding particles in a filtration circulating system as a micro-filtration medium, locally implementing porous loosened sedimentary deposit on a membrane surface of a membrane assembly, and implementing active control of a sedimentary deposit structure by load capacity and deposition density of the particles on the surface of the separating membrane; and after sewage is filtrated, collecting the micro-filtering medium backing to a system for reusing. Aiming at an ultramicro filter membrane or a membrane distillation system, the method cancels pre-stage multimedium filtration, and provides a micro-medium locally-filtrating process. By multi-medium auxiliary filtration effect of particles, the method raises a dirt accepting capability of the system, delays membrane pollution, prolongs a membrane cleaning period, and is intended to solve problems that difference of initial flux of different membranes is big, balance flux is similar, and membrane usefulness cannot fully be performed. By selection of multimedium particle sizes of the particles, the method implements auxiliary filtration layer structure control, combines a chemical separating process and design of a membrane material surface pollution layer structure, and implements the membrane filtration process of high flux.

Provided is a fuel supply system. The fuel supply system includes a fuel pump module mounted in a fuel tank and including a reservoir having a predetermined amount of fuel filled therein and a fuel pump mounted in the reservoir to suck the fuel filled in the reservoir and supply it to an internal combustion engine; a fuel supply line supplying fuel from the fuel pump module to the internal combustion engine; and a fuel return line returning the fuel from the internal combustion engine to the fuel tank, wherein the inside of the reservoir is provided with a bypass tube connecting the fuel return line to the fuel pump to directly supply the fuel returned through the fuel return line to the fuel pump through the bypass tube.

The invention belongs to the technical field of proteomics, and in particular relates to a two-dimensional conventional column array type chromatographic separation system for removing high-abundance proteins and a separation method applying the system. According to the invention, only one multi-element liquid phase pump, one ten-way valve and two six-way valves are needed for connection and on-line two-dimensional switching; and the automation degree and the integration degree of an instrument are improved. Fractions obtained by performing first-dimensional IE separation on a protein sample are directly and sequentially switched through the ten-way valve and transferred to a column head of a multi-channel second-dimensional conventional reversed-phase column for enrichment; the reversed-phase column directly performs on-line desalting and gradient dilution on the protein sample; by two-dimensional conventional chromatographic separation, the high-abundance proteins can be precisely positioned on a two-dimensional chromatogram; and therefore, the accurate removal aim is fulfilled. By the method, large-volume sample injection, concentration desalting, array type separation and positioning and removal of the high-abundance proteins can be finished; and the two-dimensional conventional column array type chromatographic separation system is very suitable for positioning, removal and high-flux analysis for the high-abundance proteins required by research on proteomics.

The invention relates to a multiple-gene detecting kit related to antitumor drugs. The multiple-gene detecting kit comprises a mixture of RT (reverse transcription) primers and a mixture of PCR (polymerase chain reaction) amplification primers, wherein each RT primer and each PCR amplification primer based on genetic groups, reference genes and a reaction internal label are respectively contained in each of the mixture of the RT primers and the mixture of the PCR amplification primers; the multiple-gene detecting kit is characterized in that the genetic groups comprise PTEN, EGFR, DPYD, HER2, RRM1, ERCC1, TUBB3, TOP1, TYMS and TOP2A; the reference genes comprise ACTB, GAPDH and B2M; the reaction internal label is KAN-rRNA (ribosomal RNA). The multiple-gene detecting kit related to antitumor drugs disclosed by the invention can be used for systemically detecting the expression level of a plurality of genes closely related to the antitumor drugs by one step, is simple and convenient to use, good in accuracy and high in detecting efficiency, and can be used for guiding chemotherapy drugs and selecting chemotherapy regimens very well.