Antibody chip reagent kit for detecting hepatoma marker

A technology of antibody chips and markers, applied in the biological field, can solve the problems of low tumor sensitivity and specificity, and achieve the effects of less specimen consumption, saving sample volume, consistency and reliability time

Active Publication Date: 2016-05-11
RAYBIOTECH INC GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of retrospective studies have found that the sensitivity and specificity of tumor markers for a single indicator are very

Method used

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  • Antibody chip reagent kit for detecting hepatoma marker
  • Antibody chip reagent kit for detecting hepatoma marker
  • Antibody chip reagent kit for detecting hepatoma marker

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1: Preparation of the kit.

[0041] 1. Screening and source of antibodies

[0042] Take the AFP antibody screening as an example, use this antibody to detect 6 clinical samples (the detection value of the clinical sample is known and measured by electrochemiluminescence method), and compare the detection value of the clinical sample with the detection value of the kit as a scatter diagram The correlation is as follows: the results of electrochemiluminescence detection are basically equivalent to those of this kit.

[0043] Take the AFP antibody as an example, use this antibody to detect 6 clinical samples (the detection value of the clinical sample is known, measured by electrochemiluminescence method, and the light absorption peak is converted into a value by software), and the clinical sample detection value is compared with this reagent The detection value of the box is compared as a scatter diagram (see figure 1 ), the correlation is as follows: the results of ele...

Example Embodiment

[0079] Example 2: The kit of the present invention is used in an experiment for detecting liver cancer tumor markers.

[0080] 1. Complete drying of the slide chip

[0081] Take out the antibody chip from the liver cancer marker detection antibody chip kit of the present invention.

[0082] The antibody chip frame can have two kinds. If one antibody spot has 4 holes (ie 4 repeats), a 2×8 chip frame can be used; if an antibody spot has 2 holes, a 4×16 chip frame can be used.

[0083] After equilibrating the glass slide chip at room temperature for 20-30 minutes, open the packaging bag, uncover the sealing strip, and then place the chip in a vacuum desiccator or dry at room temperature for 1-2 hours.

[0084] 2. Chip operation process

[0085] Add 50-100μl of sample to each well, and the amount of different samples is different: plasma and serum are diluted 1:1 with sample diluent before use; cell supernatant can be used as original solution; cell or tissue lysate is determined by protein ...

Example Embodiment

[0104] Example 3: The detection sensitivity of the antibody used in the kit of the present invention and the cross-reaction result of the antibody

[0105] A detection sensitivity of the antibody used in the kit of the present invention

[0106] Refer to Example 2 for specific operations. The lowest detection limit and linear range of the kit were obtained by using each antigen in a gradient dilution.

[0107] Table 2: Detection sensitivity of 8 antibodies in the kit of the present invention

[0108]

[0109] 2. The cross-reaction result of the antibody used in the kit of the present invention

[0110] Refer to Example 2 for specific operations. The detection antibodies are anti-AFP antibody, anti-CK19 antibody, anti-VEGF antibody, anti-DCP antibody, anti-SCCA antibody and a mixture of these five antibodies. The cross-reactivity test results of antibody pairs are as follows Picture 9 Shown.

[0111] It can be seen from the results that each antibody pair can specifically recognize its ...

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Abstract

The invention relates to an antibody chip reagent kit for detecting a hepatoma marker. The antibody chip reagent kit comprises a glass slide, a soft silicone rubber pad, a hard frame and reaction grooves formed by U-shaped frame clamps, wherein the hard frame is divided into 2*8 or 4*16 sub-boxes so as to form the 16-hole or 64-hole frame; the size of the soft silicone rubber pad corresponds to those of the hard frame and the standard glass slide; each sub-box of the hard frame forms the corresponding small reaction groove; the standard glass slide in each of the small reaction grooves is bonded with specific antibodies with corresponding concentrations; the specific antibodies are antibodies for the following 8 selected tumor markers: AFP, AFP-L3, DCP, AFU, GP73, SCCA, CK19 and VEGF. The antibody chip reagent kit adopts the antibody chip technology, so that 8 types of hepatoma markers can be detected at a time, the defect that the conventional single marker detection method is big in sample usage amount and long in detection time is avoided, and the advantages of high detection speed, less sample consumption, high sensitivity, good stability and the like are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an antibody chip kit for detecting liver cancer markers. Background technique [0002] Liver cancer is one of the most common clinical malignant tumors. The global incidence rate is increasing year by year, exceeding 626,000 / year, ranking fifth among malignant tumors; the death rate is close to 600,000 / year, ranking third among tumor-related deaths. PLC is highly prevalent in my country. At present, the number of cases in my country accounts for about 55% of the world; it ranks second only to lung cancer in tumor-related deaths. Therefore, liver cancer seriously threatens the health and life of our people. The liver is the largest substantial organ in the human body and undertakes various important metabolic functions of the human body. Therefore, once a malignant tumor occurs in the liver, it will lead to serious consequences that do not reach life. And because the liver has a rich ...

Claims

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Application Information

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IPC IPC(8): G01N33/574
Inventor 黄若磐毛应清张卓
Owner RAYBIOTECH INC GUANGZHOU
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