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Method for continuous cycle fermentation production of citric acid

A technology of cyclic fermentation and citric acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low degree of fermentation automation, large fluctuations in the quality of fermentation liquid, and large investment in fermentation equipment, so as to improve the fermentation process. Conversion rate, realization of mechanization and automation, effect of reducing equipment cleaning

Inactive Publication Date: 2013-05-15
甘肃雪晶生化有限责任公司
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Problems solved by technology

However, the traditional batch single-tank fermentation process of citric acid is insufficient. Its main problems are low fermentation automation, high labor intensity, easy contamination of bacteria, large fluctuations in the quality of the fermentation broth, low conversion rate, low equipment utilization, and large investment in fermentation equipment. serious environmental pollution

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  • Method for continuous cycle fermentation production of citric acid

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Embodiment Construction

[0018] The invention is equipped with high-efficiency combined pipeline conveying equipment, which is composed of cascaded fermentation tanks, and adopts 8 to 10 fermentation tanks in each line for single-line continuous production. The steps are as follows:

[0019] a. Blending: Mix 4t of starch milk and 2t of corn flour into the blending tank, add 24L of high-temperature amylase, start stirring, add hot water at a temperature of 45°C to set the volume to 40m 3 , use calcium oxide to adjust the pH value to 6.0;

[0020] b. Jet liquefaction: preheat the injector and laminar flow tank first, then turn on the injector, pump the adjusted slurry into the jet heater and mix it with steam. The temperature is controlled at 90°C, and the steam pressure is kept at 0.35MPa. Then enter the laminar flow tank for heat preservation, and the heat preservation time is 35 minutes to complete the liquefaction;

[0021] c. Continuous elimination and sterilization: first use a continuous pump t...

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Abstract

The invention provides a method for continuous cycle fermentation production of citric acid. The method comprises slurry mixing, injection liquefaction, continuous elimination and continuous fermentation. The method adopts high-efficiency combined pipe conveying equipment. The high-efficiency combined pipe conveying equipment comprises stepped fermentation tanks and realizes single-line continuous production, wherein each one of production lines comprises the 8-10 fermentation tanks. In a fermentation period, a back flow and a flow amount are controlled according to residual sugar consumption of fermentation liquor flowing out of the tail fermentation tank and acid production situation so that the final residual reducing sugar content is less than 0.1% and an acid yield is close to or more than an amount of post-inoculation total sugar and thus the fermentation is in a continuous fermentation state. The method overcomes the defects of the existing batch single-tank fermentation, effectively realizes mechanization and automation, reduces labor intensity, improves an equipment utilization rate and a fermentation conversion rate, does not produce environmental pollution in fermentation, and is suitable for industrial fermentation production of citric acid.

Description

technical field [0001] The invention relates to a method for producing citric acid by continuous cycle fermentation, belonging to the technical field of biological fermentation. Background technique [0002] Citric acid, also known as citric acid, its chemical name is 2-hydroxypropanetricarboxylic acid. Because of its pleasant sour taste, refreshing taste, no aftertaste, safety and non-toxicity, it has become the edible organic acid with the largest production and consumption in the world. The output and export volume of citric acid in my country has ranked first in the world for many years, accounting for about 70% of the world's total, of which 70% to 80% are used in the production of beverages and food, and the rest are used in medicine, chemical industry, environmental protection, cosmetics, textiles, Printing and dyeing, construction and other fields. [0003] At present, the industrialized fermentation production of citric acid mainly includes surface (shallow plate) ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 甘柏林刘发宏夏学鹏王顺刚周民崔建设
Owner 甘肃雪晶生化有限责任公司
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