Semicontinuous fermentation technology of gluconate

A technology of gluconate and fermentation process, applied in the field of gluconate semi-continuous fermentation process, can solve the problems of low conversion rate, many by-products, difficult to control conditions, etc., and achieve the effect of reducing the number of times and saving sugar consumption

Inactive Publication Date: 2012-01-18
山东福洋生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods of industrial production of gluconic acid and its salts both at home and abroad include: 1, chemical oxidation method, mainly refers to sodium hypochlorite oxidation method and hydrogen peroxide oxidation method, the method has many by-products, the product is difficult to separate, and the yield is low; 2. , electrolytic oxidation method, this method is to add glucose solution of certain concentration and suitable electrolyte in electrolyzer, constant current electrolysis under certain temperature and current density, this method consumes a lot of energy in industrial production, and condition is difficult to control; 3, Biological fermentation method, using fungi or bacteria to oxidize glucose into gluconic acid, this method takes a long time to ferment and the conversion rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The first step, seed culture: Add 2.5Kg of glucose, 75g of corn steep liquor, 6.4g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L culture tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.

[0011] The second step, fermentation: add 8.4Kg of glucose, 5.6g of magnesium sulfate, 0.56g of potassium dihydrogen phosphate, 2.8g of urea, and 2ml of defoamer to a 50L fermenter. At 38°C, inoculate the cultured seed solution with a 10% inoculation amount. Control the dissolved oxygen (DO) ≥ 30, the temperature is 38°C, adjust the pH to 5.5 with 20% calcium carbonate, start the fermentation, when the dissolved oxygen (DO) rises to 100, the fermentation ends, the fermentation time is 18-22 hours, the fermentation ends The hourly volume is 34-36L.

[0012] The third step, semi-continuous fermentation:

[0013] (1) Ingredients: Add 7Kg of glucose, 18g of...

Embodiment 2

[0022] The first step, seed culture: Add 2.5Kg of glucose, 75g of corn steep liquor, 6.4g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L culture tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.

[0023] The second step, fermentation: add 8.4Kg of glucose, 5.6g of magnesium sulfate, 0.56g of potassium dihydrogen phosphate, 2.8g of urea, and 2ml of defoamer to a 50L fermenter. At 38°C, inoculate the cultured seed solution with a 10% inoculation amount. Control the dissolved oxygen (DO) ≥ 30, the temperature is 38°C, adjust the pH to 5.5 with 32% sodium hydroxide, and start the fermentation. When the dissolved oxygen (DO) rises to 100, the fermentation ends. The fermentation time is 18-22 hours, and the fermentation ends The hourly volume is 34-36L.

[0024] The third step, semi-continuous fermentation:

[0025] (1) Ingredients: Add 7Kg of glucose,...

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PUM

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Abstract

The invention discloses a semicontinuous fermentation technology of gluconate, comprising the following steps: using biological fermentation process, carrying out seed cultivation, fermentation, sterilization, discoloration, condensation, crystallization, drying and other steps to let glucose be subject to fermentation to generate gluconate. In the step of fermentation, when dissolved oxygen (DO) rises to 100, 50 % of the fermentation broth in a fermentation cylinder is discharged, and the rest 50 % of the fermentation broth is used as seed and kept in the fermentation cylinder, then 650 g / L glucose liquid and nutrient are added by one time, then fermentation is carried out unceasingly; and the step of semicontinuous fermentation is started, the semicontinuous fermentation is repeated 6-10 times, and the total fermentation period is 110-130 h. The technology disclosed in the invention reduces the times of seed culture, and saves the sugar consumption in seed culture, and the yield can be increased to 115+ / -3 %.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a semi-continuous fermentation process of gluconate. Background technique [0002] Gluconic acid is widely used in food, medicine, chemical industry, water treatment, construction and other industries as bulking agent, coagulant, chelating agent and sour agent; gluconate (such as with sodium, calcium, zinc, ferrous and other metal oxides Synthetic gluconate) can be used as food additives and nutritional supplements. [0003] At present, the main methods of industrial production of gluconic acid and its salts both at home and abroad include: 1, chemical oxidation method, mainly refers to sodium hypochlorite oxidation method and hydrogen peroxide oxidation method, the method has many by-products, the product is difficult to separate, and the yield is low; 2. , electrolytic oxidation method, this method is to add glucose solution of certain concentration and suitable electrolyte in el...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/58
Inventor 赵伟张雷达王健
Owner 山东福洋生物科技股份有限公司
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