A method for converting l-alanine into d-alanine by immobilized bacillus subtilis

A technology of Bacillus subtilis and alanine, applied in the field of bioengineering, can solve the problems of high price, high cost of enzyme preparation, complicated separation and purification process, etc., and achieve the effect of reducing production cost, energy consumption and number of cultures

Active Publication Date: 2021-03-30
GUANGXI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

At present, the production and preparation methods of D-alanine mainly include microbial fermentation method, chemical asymmetric synthesis method and aminoacylase resolution method. Complicated, chemical asymmetric synthesis method is complicated, the source of chiral auxiliaries required is limited and expensive, and the cost of enzyme preparation required by aminoacylase resolution method is high, which restricts the large-scale production of D-alanine

Method used

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  • A method for converting l-alanine into d-alanine by immobilized bacillus subtilis

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Embodiment 1

[0029] (1) Preparation of cell suspension

[0030] The Bacillus subtilis seed liquid is inoculated in the fermentation medium according to the inoculum amount of 1%, (the medium consists of: 3% sucrose, 0.5% yeast extract, 0.4% L-alanine, KH 2 PO 4 0.2%, Mg 2 SO 4 .7H 2 O 0.09%, CaCl 2 0.02%, pH=6.5) were fermented and cultured in a 5L fermenter for 24 hours, the culture conditions were 30°C, the ventilation rate was 1.0vvm, the rotation speed was 600rmp, and the pH was 6.5. After the fermentation was completed, the cells were obtained by centrifugation, washed twice with deionized water, and the cells were resuspended with 9 g / L sodium chloride solution.

[0031] (2) Immobilization of microbial cells

[0032] Weigh a certain amount of polyvinyl alcohol and sodium alginate, dissolve in heated water, the final concentrations of polyvinyl alcohol and sodium alginate are 8% and 2.5%, respectively, put them into a sterilizer for sterilization at 121°C for 20min. When the ...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a method for transforming L-alanine to D-alanine by immobilized bacillus subtilis. The method for transforming L-alanine to D-alanine by immobilized bacillus subtilis comprises the steps: preparation of cell suspension, immobilization of microbial cells, L-alanine transformation by using immobilized cells, repeated use of the immobilized cells and extraction of D-alanine. The method for transforming L-alanine to D-alanine by immobilized bacillus subtilis is high in D-alanine recovery and high in purity.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for converting L-alanine into D-alanine by immobilized Bacillus subtilis. Background technique [0002] Alanine (ALa) is one of the 20 basic amino acids that make up human proteins and is an important part of life. According to the chirality and optical activity of its molecular structure, it can be divided into L-alanine and D-alanine . D-alanine is an important organic chiral source, which is widely used in the pharmaceutical industry and food production industry, such as the production of new antibiotics, vitamin B6 and new sweetener alitame. With the application and development of D-alanine in the food and pharmaceutical industries, the demand for D-alanine in the market also increases. At present, the production and preparation methods of D-alanine mainly include microbial fermentation method, chemical asymmetric synthesis method and aminoacylas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06C12N11/10C12N11/084C12R1/125
CPCC12N11/08C12N11/10C12P13/06
Inventor 黄时海张阳阳黄飞李湘萍尉晓东张彩飞李连威刘诗宇
Owner GUANGXI UNIV
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