The invention discloses escherichia coli for expressing tyrosine phenol lyase active inclusion body and an application of the escherichia coli, and belongs to the technical field of bioengineering. Escherichia coli BL21 is used as a host to express a recombinant plasmid pET-28 (a)-TPL and 9 functional oligopeptides fused with the carboxyl terminal of tyrosine phenol lyase separately, recombinant escherichia coli for producing L-DOPA is obtained, and the L-DOPA is produced through whole-cell transformation of the obtained recombinant bacteria, wherein the L-DOPA yields of the whole cell reactions of the strains TPL-ELP10, TPL-EAK16, TPL-18A and TPL-GFIL16 are 37.8 + / -1.5 g / L, 53.8 + / -2.1 g / L, 37.5 + / -0.5 g / L and 29.1 + / -1.9 g / L respectively; and compared with a control strain, the yield isimproved by 111%, 201%, 109% and 62.6% respectively.