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60 results about "Cellular Transformation" patented technology

Cellular Transformation. Cellular Transformations is a course that uses cross-disciplinary approaches, with advances in engineering and biology influencing design production and implementation. It is ever important for designers to use knowledge from other scientific fields.

Nr-CAM gene, nucleic acids and nucleic acid products for therapeutic and diagnostic uses for tumors

The present invention relates to the identification of a novel role of Nr-CAM in cell transformation and aberrant cellular proliferation. In particular, the present invention relates to the altered gene expression of Nr-CAM in a number of primary tumors and cell lines derived from tumors, in addition to, the altered gene expression of ligands for Nr-CAM. Further, the present invention relates, in part, to the Applicants' surprising discovery that the inhibition of Nr-CAM gene expression or the inhibition of Nr-CAM activity in transformed cells reverses the transformed phenotype.
Owner:MURPHY GERALD +3

Pichia kudriavzevii ZJPH0802 and application thereof in preparation of curcumin derivatives

The invention discloses Pichia kudriavzevii ZJPH0802 and an application thereof in preparation of curcumin derivatives. The strain is collected in China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuhan, China, the collection data is September 24, 2012, and the collection number is CCTCC M 2012373. The invention provides a new strain, namely the Pichia kudriavzevii ZJPH0802 for preparing the curcumin derivatives by biotransformation, and structural modification can be performed on curcumin by utilizing a strain resting cell transformation method for obtaining the corresponding derivatives; the pharmacological or biological activity of structurally modified matters of the curcumin can be improved to different extents relative to a curcumin substrate before modification so as to be conductive to development of new pharmaceutical preparations. The technology for obtaining the curcumin derivatives by adopting the biotransformation method, disclosed by the invention, is simpler and environment-friendly, a biological catalyst is a microbial thallus, and the Pichia kudriavzevii ZJPH0802 further has the advantages of self-fermentation and production, stable quality and low cost.
Owner:菏泽建数智能科技有限公司

Therapeutic and diagnostic applications based on the role of the CXCR-4 gene in tumorigenesis

The present invention relates to the identification of a novel role of CXCR-4 in cell transformation and aberrant cellular proliferation. In particular, the present invention relates to the altered gene expression of CXCR-4 in a number of primary tumors and cell lines derived from tumors, in addition to, the altered gene expression of ligands for CXCR-4. Further, the present invention relates, in part, to the Applicants' surprising discovery that the inhibition of CXCR-4 gene expression or the inhibition of CXCR-4 activity in transformed cells reverses the transformed phenotype.
Owner:NORTHWEST BIOTHERAPEUTICS INC

Anti-human papilloma virus small-molecular antibody as well as composition, preparation method and application thereof

The invention relates to an anti-human papilloma virus small-molecular antibody as well as a composition, a preparation method and application thereof. The anti-human papilloma virus small-molecular antibody Fab can enter an infected cell through a cell membrane to attack an HPV-E6/E7 oncogene protein, and paralyze and inactivate the HPV-E6/E7 oncogene protein to cause the protein to lose cell transformation and carcinogenesis. According to the anti-human papilloma virus small-molecular antibody disclosed by the invention, anti-human papilloma virus E6, E7and E6/E7 small-molecular antibodies Fab of different combinations for covering all locally popular types of HPVs can be produced according to an epidemic situation of local HPV, so as to take objective-oriented measures and realize precise prevention and treatment. The invention further provides a liquid crystal micro capsule, gel, a suppository, ointment, lotion, a tablet, an effervescent tablet, a capsule, a soft capsule, a spray and an injection which are prepared from the anti-human papilloma virus small-molecular antibody and the composition thereof. The anti-human papilloma virus small-molecular antibody is used for preventing and treating human papilloma virus (HPV) infection and/or applied to a drug, a disinfection product, a health care product and a medical instrument for preventing and treating the cervical cancer.
Owner:SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC

Immortalized quality-control cell bank for chromosome karyotype analysis and construction method thereof

The invention relates to an immortalized quality-control cell bank for chromosome karyotype analysis in the medicine field and a construction method thereof. The invention is mainly characterized in that the construction method comprises: taking residual attached primary or sub-culture living cells which are used for chromosomal disease diagnosis as needed by clinical diagnosis and treatment and are definitely diagnosed to have difficult chromosome abnormalities, introducing, through transgenosis technology, SV40LTag-pcDNA3.1(-) recombinant plasmid constructed by pcDNA3.1(-) connected by T4DNA ligase and digested by BamHI, and SV40LTag DNA amplified by PCR and separated by agarose gel electrophoresis, allowing recon to integrate with the cell DNA, screening immortalized cells integrated with recons by G418, performing subculture amplification and living cell cryopreservation, then extracting several respectively-prepared cells with different diseases, mixing the cells according to a required ratio for quality control so as to convert each original cell with a single chromosome abnormality into a cell with a plurality of easy-to-misdiagnose chromosome abnormalities with different ratios. Therefore, the difficulty and complexity for differential diagnosis and chromosome chimera diagnosis are increased; samples are easily available; and waste residual cells are converted into effective quality-control materials.
Owner:翁炳焕

Methods for reprogramming cells and uses thereof

A method of obtaining a neural multipotent, unipotent or somatic cell, including: i) providing a cell of a first type which is not a neural multipotent, unipotent or somatic cell; ii) increasing expression of at least one neural multipotent or unipotent gene regulator in the cell of a first type, to a level at which the at least one neural multipotent or unipotent gene regulator is capable of driving transformation of the cell of a first type into the neural multipotent, unipotent or somatic cell, wherein the at least one multipotent or unipotent gene regulator is Musashi1 (Msi1), Neurogenin 2 (Ngn2), or both Msi1 and Ngn2; and iii) placing or maintaining the cell in a neural cell culture medium and maintaining sufficient intracellular levels of the at least one multipotent or unipotent gene regulator for a sufficient period of time to allow a stable neural multipotent, unipotent or somatic cell to be obtained.
Owner:GENESIS TECH LTD

Method for producing L-ornithine by whole cell transformation of recombinant Corynebacterium crenatum

Arginase from Bacillus cereus is successfully expressed in C. crenatum SDNN403 through shuttle plasmid pXMJ19 between Escherichia coli and Corynebacterium crenatum. Results of enzymatic determination show that an original bacterium has no activity of the arginase; enzyme activity of the arginase of a recombinant engineering bacterium is up to 24.3 U / mg. By using a whole-cell body of the recombinant engineering bacterium as a biocatalyst and the L-ornithine as a substrate, on the basis of enzymatic properties of the arginase, an optimal reaction temperature being 40 DEG C and pH being 9.0 are determined; 200ml of LBG cultivated recombinant bacterium is resuspended in 200ml of substrate buffer solution; the L-ornithine is produced by means of transformation under optimal transformation conditions; after 8h, 149.2g / L L-ornithine is acquired; molar conversion rate of substrate L-ornithine is up to 99.5%. The L-ornithine produced by the method has the advantages of high efficiency, high specificity, low energy consumption and the like.
Owner:JIANGNAN UNIV +1

Method for producing cholest-4-en-3-one through whole-cell transformation of cholesterol by using recombinant Bacillus subtilis

The invention relates to a method for producing cholest-4-en-3-one through whole-cell transformation of cholesterol by using recombinant Bacillus subtilis and belongs to the field of genetic engineering and enzyme engineering. According to the method, the gene amplification of cholesterol oxidase (ChoM) in mycobacterium neoaurum is achieved firstly, and then, the overexpression of the gene in a type strain bacillus subtilis 168 is realized by using plasmid pMA5. According to the method, a bacillus subtilis engineering strain, which takes cholesterol as a substrate, takes whole-cell transformation as a method, is capable of highly yielding cholest-4-en-3-one is constructed, and researches on the enzyme activity and fermentation performance of the strain show that the activity of the cholesterol oxidase is obviously improved as compared with the activity of a starting strain, and the molar conversion rate of the substrate (0.1% (w/v) cholesterol) reaches 67% and 83% by carrying out whole-cell transformation for 24 hours and is 20 and 25 times the relative transformation rate of the starting strain. The method provides a beneficial guide for the industrialization of microbial one-step fermentation production of cholest-4-en-3-one.
Owner:JIANGNAN UNIV
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