Method for transforming and producing 9-OH-AD by using whole cells of recombinant corynebacterium glutamicum

A technology of Corynebacterium glutamicum and recombinant bacteria, which is applied in the field of bioengineering and biology, and can solve the problems of reduced production efficiency, long fermentation cycle, low conversion rate, etc.
CN108587997AActive Publication Date: 2018-09-28JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2018-09-28
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Abstract

The invention discloses a method for transforming and producing 9-OH-AD by using whole cells of recombinant corynebacterium glutamicum, and belongs to the technical field of bioengineering and biology. Through a shuttle plasmid pXMJ19 between escherichia coli and corynebacterium glutamicum, an oxidization subunit KshA and a reducing subunit KshB of 3-ketosteroid 9alpha-hydroxylase from Mycobacterium sp.Strain VKM Ac-1817D are successfully co-expressed in C.glutamicum ATCC13032. A whole-cell thallus of the engineering bacterium is used as a biocatalyst to transform and produce 9alpha-hydroxyl androstane-4-alkene-3,17-diketone; the mole transformation rate of the substrate androstane-4-alkene-3,17-diketone is up to 99.5 percent. The 9alpha-hydroxyl androstane-4-alkene-3,17-diketone producedby the method disclosed by the invention has the advantages of high efficiency, high specifity, low energy consumption and the like.
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Description

technical field

[0001] The invention relates to a method for transforming and producing 9-OH-AD by whole cells of recombinant Corynebacterium glutamicum, belonging to the fields of bioengineering and biotechnology. Background technique

[0002] Steroids are an important class of natural organic compounds that widely exist in biological tissues. Steroids have a variety of physiological functions and exert unique curative effects, so they are widely used in clinical practice. Steroids are the second largest class of drugs next to antibiotics, with an annual growth rate of more than 15%. Steroids usually have a series of unique physiological functions, which are mainly due to the differences in substituents, double bond positions or stereo configurations on the core of steroids. Steroids have similar structures and complex structures and are widely found in animal and plant tissues and some microorganisms. The more common ones are cholesterol, bile acid, sex hormones, adrenal ...

Claims

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