Anti-human papilloma virus small-molecular antibody as well as composition, preparation method and application thereof

A human papilloma virus, anti-human papilloma technology, applied in the preparation method of peptides, antiviral agents, antiviral immunoglobulins, etc., can solve the problems of no cervical cancer drugs

Pending Publication Date: 2018-11-20
SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an anti-human papillomavirus small molecule antibody and its composition, preparation method and application, so as to solve the problem that there is no drug that can effectively treat cervical cancer in the prior art

Method used

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  • Anti-human papilloma virus small-molecular antibody as well as composition, preparation method and application thereof
  • Anti-human papilloma virus small-molecular antibody as well as composition, preparation method and application thereof
  • Anti-human papilloma virus small-molecular antibody as well as composition, preparation method and application thereof

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Experimental program
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preparation example Construction

[0038] The preparation method of anti-human papillomavirus small molecule antibody is as follows:

[0039] Specifically, A. Preparation of antigen: Add Freund's adjuvant to human papillomavirus polypeptide, and place it in a high-speed homogenizer for high-speed homogenization to obtain human papillomavirus polypeptide antigen for immunization; wherein, human papillomavirus The polypeptide is HPV-E6, HPV-E7 or HPV-E6-E7; the mass ratio of the human papillomavirus polypeptide to Freund's adjuvant is (1-10):(10-1).

[0040] B. Prepare immunized eggs: inject the human papillomavirus polypeptide antigen for immunization once every two weeks to the laying hens, inject 2-5 times in total, and take the immunized eggs produced by the laying hens at least 12 days after the last injection , and carry out coding labeling to obtain anti-human papillomavirus-IgY immunized eggs; preferably, inject 3 times, and take the immunized eggs produced by laying hens after the 15th day after the thir...

Embodiment 1

[0104] Example 1: Anti-human papillomavirus E6 / E7 small molecule antibody Fab gel makes HPV detection positive women "positive to negative" test

[0105] (1) Subjects: women aged 21-45 years old, without major organ diseases, drug allergy history and allergy constitution), women with sexual life history, diagnosed with HPV antibody test and liquid-based cytology examination positive patients. A total of 260 cases. They were randomly divided into treatment group and control group, 130 cases each.

[0106] (2) Test method

[0107] Treatment group: treated with anti-human papillomavirus E6 / E7 small molecule antibody Fab Gynecology Gel. Wash with anti-human papillomavirus E6 / E7 small molecule antibody Fab lotion every day, and then vaginally infuse anti-human papillomavirus E6 / E7 small molecule antibody Fab gynecological gel, once a day, use one each time, each course of treatment Three boxes of 9 sticks were used continuously. Three consecutive courses of treatment. Follow-...

Embodiment 2

[0115] Example 2: Preparation of anti-human papillomavirus E6 / E7 small molecule antibody Fab liposome liquid crystal microcapsules.

[0116] The specific implementation steps are as follows:

[0117] (1) Dissolve 50% of lecithin and cholesterol in ether in a ratio of 1:5;

[0118] (2) Add the prepared anti-human papillomavirus E6 / E7 small molecule antibody Fab dry powder in a ratio of 1:100 to 4 mmol / L phosphate buffered saline (PBS) to form an antibody Fab solution with a concentration of 1%;

[0119](3) Mix the lecithin and cholesterol ether solution with the PBS solution of the antibody Fab in a ratio of 3:1;

[0120] (4) Ultrasonic treatment for 2 minutes (0.5 minutes per treatment, 0.5 minutes intermittently);

[0121] (5) Evaporate under reduced pressure in a water bath until it becomes a gel, vortex to invert the phase of the gel, and then continue to evaporate to remove the ether;

[0122] (6) Ultracentrifugation (35000r / min, 30min) to separate and remove unincluded...

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Abstract

The invention relates to an anti-human papilloma virus small-molecular antibody as well as a composition, a preparation method and application thereof. The anti-human papilloma virus small-molecular antibody Fab can enter an infected cell through a cell membrane to attack an HPV-E6 / E7 oncogene protein, and paralyze and inactivate the HPV-E6 / E7 oncogene protein to cause the protein to lose cell transformation and carcinogenesis. According to the anti-human papilloma virus small-molecular antibody disclosed by the invention, anti-human papilloma virus E6, E7and E6 / E7 small-molecular antibodies Fab of different combinations for covering all locally popular types of HPVs can be produced according to an epidemic situation of local HPV, so as to take objective-oriented measures and realize precise prevention and treatment. The invention further provides a liquid crystal micro capsule, gel, a suppository, ointment, lotion, a tablet, an effervescent tablet, a capsule, a soft capsule, a spray and an injection which are prepared from the anti-human papilloma virus small-molecular antibody and the composition thereof. The anti-human papilloma virus small-molecular antibody is used for preventing and treating human papilloma virus (HPV) infection and / or applied to a drug, a disinfection product, a health care product and a medical instrument for preventing and treating the cervical cancer.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to an anti-human papillomavirus small molecule antibody and its composition, preparation method and application. Background technique [0002] Cervical cancer is a disease that seriously endangers women's health and life. Every 2 minutes in the world, one person dies from cervical cancer. Modern medicine has proved that cervical cancer is derived from a human papillomavirus (HPV). 80% of women will be infected by HPV before the age of 50, and the number of infected women in the world reaches 3 billion. The latest research shows that among Chinese women aged 30 to 50, the total infection rate of high-risk HPV is 15% to 20.8%, that is, one of every 5-6 middle-aged women is infected with the most carcinogenic high-risk HPV, and there is morbidity. increasingly younger trend. According to statistics, the incidence of cervical cancer in my country is 6 times that of develope...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/02C07K1/36C07K1/34C07K1/18C07K1/16A61K39/42A61P31/20A61P35/00
CPCA61P31/20A61P35/00C07K16/02C07K16/084A61K2039/505C07K2317/11C07K2317/35C07K2317/55
Inventor 包晟杨荣鉴王长安曹天福龚芹
Owner SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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