Method for synthesizing D-psicose by using whole cell transformation

A technology of whole-cell transformation and psicose, applied in the biological field, can solve the problems of low product yield and difficult to completely distinguish products, and achieves the effects of improving the conversion rate, reducing the production cost, and being beneficial to industrialized production.

Pending Publication Date: 2020-08-11
DONGTAI HIRYE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reaction catalyzed by isomerase is mostly a reversible process (the conversion rate of isomerase is generally only about 30%)

Method used

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  • Method for synthesizing D-psicose by using whole cell transformation
  • Method for synthesizing D-psicose by using whole cell transformation
  • Method for synthesizing D-psicose by using whole cell transformation

Examples

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Effect test

Embodiment 1

[0022] A kind of method that utilizes whole cell to transform and synthesize D-psicose, take D-fructose as substrate (concentration of fructose is 10g / L), add ATP (add-on is 50mM), metal ion (Mg of 5mM 2+ ) and recombinant Escherichia coli induced wet thallus (the concentration of wet thallus is 20g / L), adjust the pH to 8.5, control the reaction temperature to 37°C, and catalyze the reaction for 6 hours (the entire reaction system above is 1L) After the reaction is over, heat the reaction system to 100°C, adjust the pH to about 5.0, add acid phosphatase (AP) on a shaker at 30°C overnight to remove the phosphate group, then adjust the pH to 7.0 with NaOH, and heat at 100°C To terminate the reaction, the supernatant was collected by high-speed centrifugation for 10 minutes to obtain D-psicose.

[0023] Wherein the recombinant escherichia coli is a recombinant escherichia coli expressing L-rhamnokinase and D-psicose-3-epimerase simultaneously; (in the present invention, Bacillus ...

Embodiment 2

[0038] During the catalytic reaction, the reaction temperature of the entire reaction system was 30° C., and the rest of the operations were the same as in Example 1.

Embodiment 3

[0040] During the catalytic reaction, the pH of the whole reaction system was 7.0, and all the other operations were the same as in Example 1.

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Abstract

The invention relates to the technical field of biology, and in particular to a method for synthesizing D-psicose by using the whole cell transformation. In the method, the entire reaction system comprises a substrate D-fructose, wet bacteria of recombinant Escherichia coli after induction, ATP and metal ions, the reaction system is subjected to a catalytic reaction and then is heated, cooling isperformed and then acid phosphatase is added to carry out a dephosphorylation reaction to obtain D-psicose. The yield of D-psicose can be improved by using the method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for synthesizing D-psicose by transforming whole cells. Background technique [0002] D-psicose is a rare ketohexose, which is isomer with D-fructose. Due to the remarkable efficacy of D-psicose in medical treatment and health care, it was officially listed as a GRAS (Generally Recognized as Safe) food in 2011. With the continuous advancement of research on rare sugars, D-psicose has received extensive attention because of its unique functional properties. In terms of food application, its sweetness is about 70% of that of sucrose, but its energy is low, and it has the characteristics of low absorption and low calories. It can be used as an edible substitute for sucrose. In addition, D-psicose has the functions of inhibiting obesity, lowering blood sugar, and lowering blood lipids. It is very suitable for diabetics and obese people, so it has significant effects in medical...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12N1/21C12R1/19
CPCC12P19/02C12N9/1205C12Y207/01005C12N9/90C12Y501/03C12N9/1229C12Y207/04001
Inventor 朱理平高晓冬李子杰温俊婷冯林雪陈洲陈科材
Owner DONGTAI HIRYE BIOTECH CO LTD
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