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A kind of Saccharomyces cerevisiae strain and its culture medium, culture method, preparation method and application of freeze-dried bacteria powder

A technology of Saccharomyces cerevisiae and its cultivation method, which is applied in the field of preparation of Saccharomyces cerevisiae strain and its culture medium, cultivation method, and freeze-dried bacterial powder, which can solve the problems of difficulty in ensuring safety and sanitation, unstable product quality, and long production cycle and other problems, to achieve the effect of reducing the preparation process, strong alcohol and carbon dioxide production capacity, and fast fermentation speed

Active Publication Date: 2020-04-10
XINJIANG TIANRUN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, Saccharomyces cerevisiae TR2 uses traditional methods to prepare strains, which not only has a long production cycle, that is, a long cultivation time, but also has unstable product quality, and it is difficult to ensure safety and sanitation.

Method used

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  • A kind of Saccharomyces cerevisiae strain and its culture medium, culture method, preparation method and application of freeze-dried bacteria powder
  • A kind of Saccharomyces cerevisiae strain and its culture medium, culture method, preparation method and application of freeze-dried bacteria powder
  • A kind of Saccharomyces cerevisiae strain and its culture medium, culture method, preparation method and application of freeze-dried bacteria powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of saccharomyces cerevisiae strain described in embodiment 1, described saccharomyces cerevisiae strain is Saccharomyces cerevisiae (Saccharomyces cerevisiae), and its taxonomic name is Saccharomyces cerevisiae, and bacterial strain number is TR2, and preservation unit is China Microorganism Culture Preservation Management Committee common Microbiology Center, the deposit number is CGMCC No.14964, and the deposit date is December 4, 2017. The address of the depository unit is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences.

[0046] (1) Its physical and chemical properties are:

[0047] ①Oxidation experiment

[0048] control - Acetic acid - formic acid - propionic acid - Monomethyl succinate - Succinic acid - L-proline L - L-glutamic acid - -Aspartic acid - D-gluconic acid - dextrin - Inulin + D-cellobiose - Gentiobiose - maltose + ...

Embodiment 2

[0059] The cultivation of the saccharomyces cerevisiae bacterial strain described in embodiment 1, concrete operation steps are as follows:

[0060] (1) Activation of strains:

[0061] Use an inoculation loop to pick a circle of Saccharomyces cerevisiae slant stored in a refrigerator at 4°C and draw a line on the YPD plate, place it in an incubator at 28°C and culture it upside down for 48 hours, and select Saccharomyces cerevisiae with full colony form to inoculate in the YPD liquid medium. Cultivate for 16 hours in a shaker at a temperature of 28° C. and a rotation speed of 150 r / min to obtain a seed liquid.

[0062] (2) Preparation of medium:

[0063] Weigh 53.8g of glucose, 21.7g of peptone, 10g of yeast extract, and 2.98g of potassium dihydrogen phosphate, dissolve them in 1L of pure water, heat and stir until the solution is clear and transparent, and there is no insoluble matter at the bottom, and transfer to a 3L fermenter that has been emptied ( BIOTECH-3JG) under t...

Embodiment 3

[0068] The cultivation of the saccharomyces cerevisiae bacterial strain described in embodiment 1, concrete operation steps are as follows:

[0069] (1) Activation of strains:

[0070] Use an inoculation loop to pick a circle of Saccharomyces cerevisiae slant stored in a refrigerator at 4°C and draw a line on the YPD plate, place it in an incubator at 28°C and culture it upside down for 48 hours, and select Saccharomyces cerevisiae with full colony form to inoculate in the YPD liquid medium. Cultivate for 18 hours in a shaker at a temperature of 28° C. and a rotation speed of 150 r / min to obtain a seed solution.

[0071] (2) Preparation of medium:

[0072] Weigh 53.8g of glucose, 21.7g of peptone, 10g of yeast extract, and 2.98g of potassium dihydrogen phosphate, dissolve them in 1L of pure water, heat and stir until the solution is clear and transparent, and there is no insoluble matter at the bottom, and transfer to a 3L fermenter that has been emptied ( BIOTECH-3JG) under...

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Abstract

The invention relates to a strain of Saccharomyces cerevisiae and its culture medium, a cultivation method, a preparation method and application of freeze-dried bacterial powder. A strain of Saccharomyces cerevisiae, the yeast strain is Saccharomyces cerevisiae, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.14964, and the preservation date is December 04, 2017. The invention also discloses its culture medium, culture method and preparation method of freeze-dried bacteria powder. A kind of Saccharomyces cerevisiae strain and its medium, culture method, preparation method and application of freeze-dried bacterial powder described in the present invention, this Saccharomyces cerevisiae strain has fast fermentation speed, strong ability of producing alcohol and carbon dioxide, and is not easy to be polluted by miscellaneous bacteria The culture medium and culture method are suitable for the bacterial strain; the preparation method of the freeze-dried bacterial powder of the bacterial strain is simple, the prepared freeze-dried bacterial powder can reduce the preparation link of the starter, and the obtained freeze-dried bacterial powder can be used for milk beer , whey fermented beverages, fruit and vegetable fermented beverages, special milk fermented products and health food.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a strain of Saccharomyces cerevisiae and its culture medium, a cultivation method, a preparation method and application of freeze-dried bacterial powder. Background technique [0002] Milk beer is a new type of sour, refreshing, nutritious alcoholic milk drink fermented by special lactic acid bacteria and yeast. Its alcohol content is less than 0.5%, milky white, with a rich malt fragrance, slightly carbonic acid, rich and white foam, fine and smooth texture; at the same time, milk beer has the characteristics of sweet and sour milk drinks, palatable and nutritious. [0003] At present, the research and application of yeast are mostly in bread, wine and biopharmaceuticals, and there are few researches on yeast for fermented dairy products. The applicant previously announced a preparation method and application (CN 107034149A) of a freeze-dried bacterial agent of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12C11/00C12R1/865
CPCC12C11/00C12N1/18C12N1/185C12R2001/865
Inventor 李新玲牟光庆罗晓红姜淑娟尤宏蒋艾廷史亚丽
Owner XINJIANG TIANRUN BIOTECH
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