Lactobacillus plantarum strain JP-8 and its application
A technology of Lactobacillus plantarum and JP-8, which is applied in the field of food microorganisms and food processing, can solve problems such as difficult long-term storage, and achieve the effect of retaining flavor and nutrients, unique flavor and high nutritional value
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Embodiment 1
[0034] Embodiment 1, utilize plant lactobacillus ( Lactobacillus plantarum ) JP-8 Process for fermenting sea asparagus puree
[0035] Take the glycerin-preserved strain of Lactobacillus plantarum JP-8 of the present invention, inoculate it on a fresh MRS solid plate, activate and cultivate it at 25°C for 48 h; use a sterilized toothpick to pick a single colony of JP-8 and inoculate it into a In a test tube containing 10 mL MRS liquid culture medium, culture it statically at 30°C for 36 h; take the cultured bacterial solution and transfer it to a 100 mL MRS liquid medium containing 50 mL MRS liquid medium at a ratio of 5% (v / v). mL Erlenmeyer flasks, cultured at 35°C for 24 h, and used as seed bacterial solution for later use.
[0036] Such as figure 1 As shown, select fresh sea asparagus twigs, blanch at 95°C for 4 minutes, then put them into tap water for desalination for 8 hours, change the water every 2 hours during this period, and finally remove and control the water....
Embodiment 2
[0038] Embodiment 2, utilizing plantaractobacillus ( Lactobacillus plantarum ) JP-8 Process for fermenting sea asparagus puree
[0039] Take the glycerol-preserved strain of Lactobacillus plantarum JP-8 of the present invention, inoculate it on a fresh MRS solid plate, activate and cultivate it at 30°C for 36 h; use a sterilized toothpick to pick a single colony of JP-8 and inoculate it into a In a test tube containing 10 mL of MRS liquid culture medium, culture it statically at 25°C for 48 h; take the cultured bacterial solution and transfer it to a 100 mL MRS liquid medium containing 50 mL MRS liquid medium at a ratio of 1% (v / v). mL Erlenmeyer flasks, cultured at 30°C for 36 h, and used as seed bacterial solution for later use.
[0040] Such as figure 1 As shown, fresh sea asparagus twigs were selected, blanched at 100 °C for 3 min, then put into tap water for desalination for 6 h, during which time the water was changed every 2 h, and finally the water was taken out an...
Embodiment 3
[0042] Embodiment 3, utilizing plantaractobacillus ( Lactobacillus plantarum ) JP-8 Process for fermenting sea asparagus puree
[0043] Take the glycerol-preserved bacterial strain of Lactobacillus plantarum JP-8 of the present invention, inoculate it on a fresh MRS solid plate, activate and cultivate it at 35°C for 24 h; use a sterilized toothpick to pick a single colony of JP-8 and inoculate it into a In a test tube containing 10 mL of MRS liquid culture medium, culture it statically at 35°C for 24 h; take the cultured bacterial solution and transfer it to a 100 mL MRS liquid medium containing 50 mL of MRS liquid medium at a ratio of 2.5% (v / v). mL Erlenmeyer flasks, cultured at 25°C for 48 h, and used as seed bacterial solution for later use.
[0044] Such as figure 1 As shown, fresh sea asparagus twigs were selected, blanched at 90°C for 6 min, then put into tap water for desalination for 10 h, during which time the water was changed every 2 h, and finally the water wa...
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