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Fermentation method for producing L-isoleucine

A fermentation method and isoleucine technology, which are applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of accumulation of heteroacids and increase in the level of heteroacids, and achieve inhibition of accumulation of heteroacids and reduction of impurities. Accumulation of acid, effect of reducing flux

Active Publication Date: 2020-05-19
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Maintaining a certain level of residual sugar during the fermentation process can maximize the release of the metabolic capacity of the bacteria and maintain a high fermentation intensity, but at the same time it will lead to the continuous accumulation of miscellaneous acids, especially in the middle and late stages of fermentation, when the metabolic activity of the bacteria gradually decreases , the intracellular metabolic pathway changes, and the level of heteroacids will increase significantly and uncontrollably

Method used

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  • Fermentation method for producing L-isoleucine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: A fermentation method for producing L-isoleucine. The cultured Corynebacterium glutamicum seed solution is inserted into a 50L fermenter with an inoculation amount of 15%, and the initial volume is 20L. The medium used for seeds consists of: glucose 20g / L, corn steep liquor 60g / L, potassium chloride 0.5g / L, magnesium sulfate 0.5g / L, defoamer 0.3mL / L, biotin 0.3mg / L, VB1 2mg / L, the rest is water. The medium composition used for fermentation is: glucose 80g / L, corn steep liquor 30g / L, ammonium sulfate 10g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, defoamer 0.3mL / L, biotin 0.1 mg / L, VB1 0.1mg / L, the rest is water. The fermentation conditions are: tank pressure 0.05-0.1Mpa, ventilation rate 10-25L / min, stirring 200-800rpm, temperature 31-33°C, pH 6.8-7.2. Control the residual sugar concentration in the fermentation broth below 0.5g / L, and maintain the sugar consumption rate at 6g / L / h. The speed of L / h gradually reduces the sugar consump...

Embodiment 2

[0016] Embodiment 2: A kind of fermentation method of producing L-isoleucine, its difference with embodiment 1 is: sugar consumption rate is maintained at 7g / L / h, when miscellaneous acid (comprising alanine, lysine etc.) when the content reaches 1g / L, gradually reduce the sugar consumption rate until the tank is put at a speed of 0.4g / L / h, and all the other are identical to Example 1.

[0017] This example was repeated 3 times. After the fermentation was completed, the content of L-isoleucine and the content of heteroacids in the fermentation broth were measured by HPLC. The fermentation indicators are shown in Table 1 below.

Embodiment 3

[0018] Embodiment 3: A kind of fermentation method of producing L-isoleucine, its difference with embodiment 1 is: sugar consumption rate is maintained at 7g / L / h, when miscellaneous acid (comprising alanine, lysine etc.) content reaches 2g / L, gradually reduces sugar consumption rate with the speed of 0.4g / L / h until putting tank, and all the other are identical with embodiment 1.

[0019] This example was repeated 3 times. After the fermentation was completed, the content of L-isoleucine and the content of heteroacids in the fermentation broth were measured by HPLC. The fermentation indicators are shown in Table 1 below.

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Abstract

The invention discloses a fermentation method for producing L-isoleucine. The method comprises seed culture and fermentation culture, wherein in the fermentation culture process, the concentration ofresidual sugar in fermentation liquor is controlled below 0.5g / L, and the sugar consumption rate is maintained below the maximum sugar consumption capacity of thalli; when heteroacid starts to be accumulated rapidly, the sugar consumption rate is gradually reduced until the fermentation liquor is discharged out of a fermentation tank. The method is simple and easy to implement, accumulation of theheteroacid is obviously inhibited, accumulation of the heteroacid is further reduced while the acid production level is increased, the acid yield of the 50-L tank reaches 44.7 g / L, and the total heteroacid is only 3.5 g / L.

Description

Technical field: [0001] The invention belongs to the technical field of L-isoleucine production, in particular to a fermentation method for producing L-isoleucine. Background technique: [0002] L-isoleucine, also known as "L-isoleucine", belongs to branched chain amino acid (BCAA), and is one of the eight essential amino acids that humans and animals cannot synthesize themselves and must be supplied from sources. It has a variety of physiological functions and is the raw material for the synthesis of human hormones and enzymes. It has the effect of promoting protein production and inhibiting its decomposition, and plays an important role in human life activities. Therefore, it has wide application and commercial value in the food and pharmaceutical industries. . [0003] As an important food additive, L-isoleucine can adjust the amino acid balance in food. It is often used to prepare various sports and fitness nutrition products, amino acid functional drinks, etc. It is al...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/06C12R1/15
CPCC12N1/20C12P13/06
Inventor 高鹏吴涛白鑫常利斌龚华李岩赵津津
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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