The invention relates to a selenium-rich needle mushroom cultivation base which is prepared from the following component raw materials: by weight, 30-40 parts of fruit particles, 20-30 parts of luffa vine, 10-12parts of walnut shell, 8-10 parts of cotton seed hull, 12-15 parts of vinasse, 10-12 parts of konjac flour, 5-8 parts of raw meal, 2-3 parts of garlic skin, 1-2 parts of chilli seed, 1-2 parts of radix changii powder, 1-2 parts of dye yam powder, 1-2 parts of ficus pumila powder, 1-3 parts of frozen green leaf powder, 1-3 parts of white hyacinth bean powder, 2-3 parts of seabuckthorn powder, 1-2 parts of setose thistle powder, 4-5 parts of spodumene powder, 0.5-1 part of sodium selenite, 0.3-0.5 part of calcium carbonate, 1-3 parts of quicklime, 18-19 parts of dashi, 2-4 parts of gypsum powder and 2-3 parts of nutritive soil. Needle mushroom cultivated by the cultivation base has the characteristics of high contents of minerals, trace elements, vitamin B, vitamin C and amino acids, can reduce heavy metal pollution, has the stomach invigorating effect by long term consumption, and is quick in growth speed and high in yield.