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Enzymatic hydrolysis method for cellulosic substance

A technology for cellulosic substances and cellulose, which is applied in the field of continuous enzymatic hydrolysis of cellulosic substances to generate fermentable sugar, can solve the problems of harsh acid hydrolysis conditions, low sugar recovery rate and high sugar cost, and achieves improved recovery rate and reduced hydrolysis. Loss of sugar, effect of improving yield

Active Publication Date: 2012-05-30
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a method for enzymatically hydrolyzing cellulose substances, which overcomes the disadvantages of harsh acid hydrolysis conditions and low sugar recovery rate, as well as the consumption of sugar by bacteria and the enzymatic high cost issues

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  • Enzymatic hydrolysis method for cellulosic substance
  • Enzymatic hydrolysis method for cellulosic substance
  • Enzymatic hydrolysis method for cellulosic substance

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Embodiment Construction

[0021] Such as figure 1 As shown, the equipment used in the process of enzymatic hydrolysis of cellulosic substances of the present invention is as follows: cellulosic substance suspension storage tank I, cellulosic substance hydrolysis tank II, cellulosic substance solid residue natural settling tank III, fiber in the hydrolysis sugar solution Cellulase recovery unit IV, cellulosic solid residue washing natural settling tank V, cellulosic solid residue separation device VI, cellulase storage tank VII, cellulase sterilization device VIII, cellulosic material suspension preparation and disinfection tank IX, cellulosic substance solid residue water washing tank X, hydrolysis sugar liquid concentration device XI, a total of 11 units, each unit can be sealed, and connected with pipelines, the whole device can be steam sterilized and kept sterile.

[0022] The cellulosic substance suspension storage tank I has a stirring device, which can keep the cellulosic substance in a relative...

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Abstract

The invention discloses an enzymatic hydrolysis method for a cellulosic substance. The enzymatic hydrolysis method for the cellulosic substance comprises the following steps: firstly, preparing and disinfecting a cellulosic substance suspension; then, mixing the cellulosic substance suspension with a sterilized cellulosic enzyme and a sterilized cellulosic enzyme promoter for a hydrolysis reactionunder hydrolysis conditions; separating hydrolysate into a solid phase and a liquid phase and washing the solid phase for further treatment and utilization; and using filter substances obtained by the ultrafiltration treatment of the liquid phase to further concentrate a hydrolysis sugar solution and recycling trapped substances obtained by the ultrafiltration treatment in the hydrolysis processbecause of the cellulosic enzyme content contained in the trapped substance. The method overcomes the defects of strict acid hydrolysis conditions and low sugar recovery rate of the prior art and solves the problems of overhigh sugar consumption by infected bacteria, overhigh enzyme cost, and the like in the enzymatic hydrolysis process. The method can be also used for the technological process for preparing ethanol by using the cellulosic substance as a raw material.

Description

technical field [0001] The invention relates to a method for hydrolyzing cellulose substances, in particular to a new process for producing fermentable sugar by continuously enzymatically hydrolyzing cellulose substances. Background technique [0002] Cellulosic hydrolysis is an important step in the production of fuel ethanol from agricultural and forestry wastes. The traditional method of hydrolysis of cellulose is acid hydrolysis, which can be divided into dilute acid hydrolysis and concentrated acid hydrolysis. The dilute acid hydrolysis method was first proposed by the Frenchman Melsens in 1856. In 1898, Germany adopted this method to produce ethanol from wood as raw material and realized industrialization. This is the oldest process of biomass hydrolysis. If acid hydrolysis is completed in only one step, the monosaccharides generated during the hydrolysis process are easily converted into other chemical substances, such as furfural, which will not only reduce the sugar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/00
Inventor 张全佟明友黎元生唐开宇唐似茵尹佩林
Owner CHINA PETROLEUM & CHEM CORP
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