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Preparation method of domestication agent for active sludge in salt-containing wastewater treatment

A technology for activated sludge and saline wastewater, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. Low efficiency and other problems, to achieve the effect of balancing external osmotic pressure, high specific surface area, and strong adaptability

Inactive Publication Date: 2018-03-23
常州文诺纺织品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention: Aiming at the problems of low salt tolerance, poor salinity impact resistance and low treatment efficiency in high-salt environment of ordinary salinity-acclimated activated sludge, a solution for the treatment of saline wastewater is provided. Preparation method of acclimation agent for activated sludge

Method used

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  • Preparation method of domestication agent for active sludge in salt-containing wastewater treatment

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Experimental program
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Effect test

Embodiment 1

[0040] Peanut shell biochar: purchased from Henan Sanli Company.

[0041] Raw materials for halophilic bacteria extraction: cured meat, purchased at farmers' markets.

[0042] Raw materials for denitrifying bacteria extraction: The sludge was collected from the high-salt wastewater near a pickle factory.

[0043] Plate culture medium for isolation and purification of halophilic bacteria: in parts by mass, take 40 parts of NaCl, anhydrous MgSO 4 9 parts, 2 parts sodium citrate, 2 parts KCl, anhydrous CaCl 2 0.2 parts, 10 parts of peptone, 2 parts of yeast extract, 20 parts of agar, 1000 parts of water, pH7.5±0.2, sterilized at 121°C for 15 min.

[0044] Halophilic bacteria enrichment liquid medium: remove the agar component in the halophilic bacteria isolation and purification plate medium, and keep other components unchanged.

[0045] Nitrifying bacteria enrichment medium: by mass fraction, take 12 parts of peptone, 6 parts of NaCl, KNO 3 1 part, 2 parts of sodium succi...

Embodiment 2

[0053] Peanut shell biochar: purchased from Henan Sanli Company.

[0054] Raw materials for halophilic bacteria extraction: cured meat, purchased at farmers' markets.

[0055] Raw materials for denitrifying bacteria extraction: The sludge was collected from the high-salt wastewater near a pickle factory.

[0056] Plate culture medium for isolation and purification of halophilic bacteria: in parts by mass, take 45 parts of NaCl, anhydrous MgSO 4 10 parts, 2 parts sodium citrate, 3 parts KCl, anhydrous CaCl 2 0.3 parts, 13 parts of peptone, 3 parts of yeast extract, 23 parts of agar, 1000 parts of water, pH7.5±0.2, sterilized at 121°C for 15 min.

[0057] Halophilic bacteria enrichment liquid medium: remove the agar component in the halophilic bacteria isolation and purification plate medium, and keep other components unchanged.

[0058] Nitrifying bacteria enrichment medium: by mass fraction, take 13 parts of peptone, 7 parts of NaCl, KNO 3 1 part, 2 parts of sodium succ...

Embodiment 3

[0066] Peanut shell biochar: purchased from Henan Sanli Company.

[0067] Raw materials for halophilic bacteria extraction: cured meat, purchased at farmers' markets.

[0068] Raw materials for denitrifying bacteria extraction: The sludge was collected from the high-salt wastewater near a pickle factory.

[0069] Plate culture medium for isolation and purification of halophilic bacteria: in parts by mass, take 50 parts of NaCl, anhydrous MgSO 4 11 parts, 3 parts sodium citrate, 5 parts KCl, anhydrous CaCl 2 0.5 parts, 15 parts of peptone, 5 parts of yeast extract, 25 parts of agar, 1000 parts of water, pH7.5±0.2, sterilized at 121°C for 15 min.

[0070] Halophilic bacteria enrichment liquid medium: remove the agar component in the halophilic bacteria isolation and purification plate medium, and keep other components unchanged.

[0071] Nitrifying bacteria enrichment medium: by mass fraction, take 15 parts of peptone, 8 parts of NaCl, KNO 3 2 parts, 3 parts of sodium suc...

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Abstract

The invention belongs to the field of biochemical treatment of industrial wastewater and in particular relates to a preparation method of a domestication agent for active sludge in salt-containing wastewater treatment. According to the preparation method provided by the invention, efficient halophilic bacteria is screened from salted meat; a halophilic strain is added to improve the composition ofmicroorganisms, so that the activity of a reaction main body active sludge is enhanced; the strain has a special salt-resisting mechanism, so that the strain is suitable for growing in a high-salt environment and degrading organic matters; the halophilic bacteria can be used for directly absorbing betaine, tetrahydropyrimidine and the like from the environment and also can be automatically synthesized in cells by absorbing substrates; the microorganisms can resist changes of osmotic pressure in the high-salt environment by adopting the mechanism, so that the microorganisms have relatively strong adaptability and the salinity impact resisting capability is improved.

Description

technical field [0001] The invention belongs to the field of biochemical treatment of industrial wastewater, and in particular relates to a domestication method of activated sludge for saline wastewater treatment. Background technique [0002] High-salt wastewater refers to wastewater with a mass fraction of inorganic salts greater than 1%. Its sources include: (1) seawater utilization: wastewater generated from seawater processing, mariculture, and seawater substitute discharge; (2) industrial wastewater: food, petroleum, chemical industry , printing and dyeing, papermaking, leather, pharmaceutical, fermentation and other industries widely produce high-salt and high-concentration organic wastewater; (3) Others: ship ballast water, salt water generated by minimizing waste water (garbage leachate, reverse osmosis concentrated drainage), Domestic sewage from large ships. In coastal areas, if the organic matter and nutrients in high-salt wastewater can be reduced below the lim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/20C02F3/12
CPCC02F3/12C12N1/20C12N11/14Y02W10/10
Inventor 黄淑枝李璐尹凯欣
Owner 常州文诺纺织品有限公司
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