A method for microbial fermentation and purification of tea saponin
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of microbial fermentation and tea saponin, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of high safety production requirements, high methanol toxicity, and large usage, so as to shorten the production cycle, Low production cost and reduced usage
Active Publication Date: 2016-01-20
HANGZHOU TEA RES INST CHINA COOP
View PDF4 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The organic solvent used in alcohol precipitation is generally methanol or ethanol, both of which are flammable and explosive, and methanol is highly toxic and requires high safety production
However, when ethanol is used, the general usage is very large, and there is a problem of large investment.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] Example 1: Pulverize the tea seed cake, pass through an 80-mesh sieve, weigh 150g of the pulverized tea seed powder, add it to 1500mL of 40°C water for ultrasonic extraction for 40 minutes, centrifuge to remove the precipitate, and prepare the supernatant for inoculation 50mL of good microbial fermentation yeast liquid, covered with gauze, let stand for 3 days, centrifuged to get the flocculation sediment, leached the sediment with 80% ethanol solution, centrifuged, the supernatant was evacuated and rotary evaporated, and the concentrated solution was sprayed After drying, the purity of tea saponin was measured to be (80%).
Embodiment 2
[0021] Example 2: Extract the pulverized Camellia oleifera seed powder with water for 40 minutes according to mass (g): volume (mL) = 1:10, filter to get the supernatant, and inoculate the prepared microbial fermentation yeast liquid with a volume ratio of 1:1 , let it stand for 2 days, take the supernatant, add fresh tea seed powder extract at a volume ratio of 1:1, and continue to ferment; such a cycle constitutes a semi-continuous fermentation. The flocs in the lower layer are centrifuged to obtain a precipitate and a supernatant, which is sterilized and then added to the rough extraction process of tea seed powder; the precipitate is leached with an ethanol solution with a mass concentration of 80%, centrifuged, and the supernatant is evacuated and rotated to evaporate. The concentrated solution was spray-dried, and the purity of tea saponin was measured to be (85%).
[0022] The microorganisms in the fermented yeast liquid used in Examples 1 and 2 are mainly microorganism...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
melting point
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses a method for purifying tea saponin by microbial fermentation, which is characterized by comprising the following steps: by using a water solution containing tea saponin as a fermentation substrate, inoculating a microbial flocculant generating bacterium capable of enabling the tea saponin to generate flocculating precipitate into the fermentation substrate, culturing until the tea saponin generates flocculation, carrying out centrifugal separation on the flocculating precipitate, extracting the tea saponin in the precipitate with ethanol to obtain the purified tea saponin ethanol solution, concentrating, and drying to obtain the refined tea saponin. The purification method can solve the problem of purification of tea saponin, and has the characteristics of safe production process and low production cost. The method has the advantages of simple technique, high operability and high product safety, and only uses the ethanol in the whole purification process. The tea saponin produced by the technique has favorable application prospects in the fields of daily use chemicals and health food.
Description
technical field [0001] The invention relates to a method for purifying tea saponin, in particular to a method for purifying tea saponin by microbial fermentation. Background technique [0002] Tea saponin, also known as tea saponin, is a kind of glycoside compound extracted from tea tree seeds. It is a kind of natural surfactant with good performance. It can be widely used in light industry, chemical industry, pesticide, feed, breeding, textile , oil extraction, mining, building materials and highway construction and other fields, manufacturing emulsifiers, detergents, pesticide auxiliaries, feed additives, crab and shrimp breeding protection agents, textile auxiliaries, oilfield foam agents, mining flotation agents and aerated concrete Foam stabilizer and concrete admixture - antifreeze, etc. [0003] Tea saponin belongs to triterpenoid saponin, has a bitter and pungent taste, stimulates the nasal mucosa to cause sneezing, the pure product is white fine columnar crystal, s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more