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Culture medium efficiently culturing chicken coryza haemophilus paragallinarum and preparation method and application of culture medium

A technology of Haemophilus gallinaceus and chicken rhinitis, applied in the field of culture medium, can solve the problems of complex preparation methods, high cost, and high price, and achieve the effects of simple formula, cost reduction, and cost reduction

Inactive Publication Date: 2017-11-07
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing medium for cultivating Haemophilus paragallinitis contains expensive and difficult to prepare sterile chicken serum, and its preparation method is complicated and costly.

Method used

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  • Culture medium efficiently culturing chicken coryza haemophilus paragallinarum and preparation method and application of culture medium
  • Culture medium efficiently culturing chicken coryza haemophilus paragallinarum and preparation method and application of culture medium
  • Culture medium efficiently culturing chicken coryza haemophilus paragallinarum and preparation method and application of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] High-efficiency culture medium of Haemophilus paragallinitis chicken rhinitis, its formula is as follows: aseptic chicken embryo allantoic fluid 0.2g / L, yeast extract powder 0.6g / L, casein hydrolyzate 0.6g / L, solvent is water.

[0021] The preparation method of high-efficiency cultivation of Haemophilus paragallinitis liquid medium, take 0.6g of yeast extract powder and 0.6g of casein hydrolyzate dissolved in 1L of distilled water, mix well, sterilize under high pressure at 121°C for 20-30 minutes, and wait for natural When the temperature is lowered to below 30°C, 0.2 g of sterile chicken embryo allantoic fluid is aseptically added to adjust the pH value to 7.1.

Embodiment 2

[0023] High-efficiency cultivation of Haemophilus paragallinarum liquid medium, its formula is as follows: sterile chicken embryo allantoic fluid 0.1g / L, yeast extract powder 0.5g / L, casein hydrolyzate 0.5g / L, solvent is water.

[0024] The preparation method of high-efficiency cultivation of Haemophilus paragallinitis liquid medium, take 0.5g of yeast extract powder and 0.5g of casein hydrolyzate dissolved in 1L of distilled water, mix well, sterilize under high pressure at 121°C for 20-30 minutes, and wait for natural When the temperature is lowered to below 30°C, 0.1 g of sterile chicken embryo allantoic fluid is aseptically added to adjust the pH value to 7.2.

Embodiment 3

[0026] High-efficiency culture of Haemophilus paragallinitis liquid medium, its formula is as follows: sterile chicken embryo allantoic fluid 1g / L, yeast extract powder 1g / L, casein hydrolyzate 0.8g / L, solvent is water.

[0027] The preparation method of high-efficiency cultivation of Haemophilus paragallinitis liquid medium, take 1g of yeast extract powder and 0.8g of casein hydrolyzate dissolved in 1L of distilled water, mix well, autoclave at 121°C for 20-30 minutes, wait for natural cooling When the temperature is below 30°C, add 1 g of sterile chicken embryo allantoic fluid aseptically, and adjust the pH value to 7.3.

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PUM

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Abstract

The invention relates to a culture medium efficiently culturing chicken coryza haemophilus paragallinarum and a preparation method and application of the culture medium. The culture medium is scientific and reasonable in formula, chick embryo allantoic fluid replaces serum, the cost can be greatly lowered, growth of the culture medium culturing the chicken coryza haemophilus paragallinarum is facilitated, compared with other culture media sold on the market, the cost is lowered by 20%-30%, the number of growing chicken coryza haemophilus paragallinarum thalli is increased by 5%-500%, and the culture medium can be widely used for culturing the chicken coryza haemophilus paragallinarum.

Description

technical field [0001] The invention belongs to the field of culture medium, in particular to a medium for efficiently cultivating Haemophilus paragallinitis gallinarum, and also relates to a preparation method and application of the medium. Background technique [0002] Haemophilus paragallinarum is a Gram-negative bacillus, which is one of the important opportunistic pathogenic bacteria and hospital-acquired infection bacteria. Haemophilus paragallinitis caused by it is a contact infectious disease that harms poultry and wild birds such as chickens, turkeys and pheasants. The morbidity and mortality of the disease are usually 10 to 30%, but some chicken farms infected with Haemophilus paragallinitis have a mortality rate as high as 75%. Once Haemophilus paragallinitis infection occurs in chicken flocks in a certain area, the disease will become an endemic disease, which is difficult to completely eradicate, and often breaks out repeatedly, causing huge economic losses. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 杨金龙戴茜茜殷素会
Owner CHONGQING ACAD OF ANIMAL SCI
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