Haemophilus paragallinarum type b strain fermentation medium, its preparation method and application

A technology of Haemophilus gallinarum and fermentation medium, which is applied in the field of fermentation medium of Haemophilus paragallinarum B type strain, can solve the problems of unsuitable B type strain growth, long adaptation period of bacteria body, slow growth and the like, and achieves shortening The effect of bacterial adaptation period, strong bacterial adaptability and high bacterial content

Active Publication Date: 2019-08-27
山东滨州沃华生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation process using commercial TSB medium, it was found that the bacteria have a long adaptation period, slow growth, and high cost, which is not suitable for the growth of type B strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Medium formula components and ratio:

[0030] Casein peptone 30g, yeast powder 10g, polyvalent peptone 10g, sodium chloride 10g, sodium glutamate 10g, fish meal peptone 8g, glucose 20g, trace element solution 10mL, chicken serum 30mL, coenzyme (NAD solution) 10mL, water , 10M sodium hydroxide solution to adjust the pH to 7.3.

[0031] Wherein, the trace element solution is 2g ferrous sulfate heptahydrate, 1.8g magnesium chloride, 1.4g zinc sulfate heptahydrate, 2.1g calcium chloride, 50g of 36% concentrated hydrochloric acid, and dilute to 1L of the obtained solution. The concentration of coenzyme (NAD solution) is 1.0g / L.

[0032] Culture medium is prepared by the following method:

[0033] 1) Dissolve casein peptone, yeast powder, polyvalent peptone, NaCl, sodium glutamate, and fish meal peptone, add trace element solution, add water, set the volume to 1000mL, raise the temperature to 37°C, adjust the pH to 7.3, and set the temperature at 121°C Autoclave for 20 min...

Embodiment 2

[0039] Medium formula components and ratio:

[0040] Casein peptone 15g, yeast powder 8g, polyvalent peptone 8g, sodium chloride 10g, sodium glutamate 10g, fish meal peptone 5g, glucose 15g, trace element solution 50mL, chicken serum 30mL, coenzyme (NAD solution) 10mL, water, 10M Sodium hydroxide solution adjusted to pH 7.4.

[0041] Wherein, the trace element solution is 2g ferrous sulfate heptahydrate, 1.8g magnesium chloride, 1.4g zinc sulfate heptahydrate, 2.1g calcium chloride, 50g of 36% concentrated hydrochloric acid, and dilute to 1L of the obtained solution. The concentration of coenzyme (NAD solution) is 1.0g / L.

[0042] Culture medium is prepared by the following method:

[0043] 1) After dissolving casein peptone, yeast powder, polyvalent peptone, NaCl, sodium glutamate, and fish meal peptone, add trace element solution, add water, set the volume to 1000mL, raise the temperature to 37°C, adjust the pH to 7.4, and set the temperature at 121°C Autoclave for 20 min...

Embodiment 3

[0049] Medium formula components and ratio:

[0050] Casein peptone 15g, yeast powder 5g, polyvalent peptone 5g, sodium chloride 10g, sodium glutamate 10g, fish meal peptone 5g, glucose 15g, trace element solution 5ml, chicken serum 30mL, coenzyme (NAD solution) 5mL, water, 10M Sodium hydroxide solution adjusted to pH 7.2.

[0051]Wherein, the trace element solution is 2g ferrous sulfate heptahydrate, 1.8g magnesium chloride, 1.4g zinc sulfate heptahydrate, 2.1g calcium chloride, 50g of 36% concentrated hydrochloric acid, and dilute to 1L of the obtained solution. The concentration of coenzyme (NAD solution) is 1.0g / L.

[0052] Culture medium is prepared by the following method:

[0053] 1) After dissolving casein peptone, yeast powder, polyvalent peptone, NaCl, sodium glutamate, and fish meal peptone, add trace element solution, add water, set the volume to 1000mL, raise the temperature to 37°C, adjust the pH to 7.4, and set the temperature at 121°C Autoclave for 20 minute...

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Abstract

The invention discloses a haemophilus paragallinarum B strain fermentation medium, a preparation method thereof and application thereof, and belongs to the field of agricultural microorganism. The haemophilus paragallinarum B strain fermentation medium is prepared from 5 to 100 g / L of casein peptone, 0.5 to 80 g / L of yeast powder, 0.5 to 80 g / L of polyvalent peptone, 0.5 to 20 g / L of sodium chloride, 0.5 to 20 g / L of sodium glutamate, 0.5 to 30 g / L of glucose, 0.5 to 30 g / L of fish meal peptone, 5 to 100 mL / L of chicken serum, 0.5 to 50 mL / L of microelement solution and 1 to 30 mL / L of NAD (Nicotinamide Adenine Dinucleotide) solution. The haemophilus paragallinarum B strain fermentation medium disclosed by the invention contains all nutritional ingredients which are needed for growth of haemophilus paragallinarum B strains, growth and metabolism of the strains are facilitated, the adaption period of thallus is shortened, the adaptive capacity of the thallus after inoculation is strong, the fermentation period is short, the viable bacteria quantity during harvesting is high, and the haemophilus paragallinarum B strain fermentation medium is suitable for large-scale application and has a wide prospect.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a fermentation medium for Haemophilus paragallinarum type B strain. Background technique [0002] Haemophilus paragallinarum, as the pathogenic bacterium of chicken infectious rhinitis, can cause upper respiratory tract infection in chickens. When the disease breaks out, it can cause a decrease in egg production of laying hens, hinder the growth and development of pregnant chickens, and increase the elimination rate. cause large losses, etc. [0003] According to Page's typing method, Haemophilus paragallinarum can be divided into three serotypes: A, B, and C, and there is no cross-protection between each type. According to the epidemiological investigation, it is found that the prevalence of type B strains is relatively wide. [0004] At present, there are many research reports on type A in the literature, and some achievements have been made, but there are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/21
Inventor 朱杰王楠牛成明董新荣
Owner 山东滨州沃华生物工程有限公司
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