54 results about "Casein peptone" patented technology

The invention discloses bacillus subtilis with phosphate-solubilizing effect and application thereof, and relates to bacillus subtilis XF-8 (CCTCC No: M2010182) and a method for producing neutral phytase from the bacillus subtilis XF-8. The application comprises the following steps: inoculating a strain in an LB culture medium, and carrying out shake culture overnight at the temperature of 30 DEG C; inoculating the strain in an enzyme-producing culture medium with 1-5% of inoculation amount, and carrying out shake culture at the temperature of 25-37 DEG C for 2-4 days, wherein, the pH value is 5.5-7.5; centrifuging at the speed of 5000rpm at the temperature of 4 DEG for 15 minutes, and taking supernatant to obtain crude neutral phytase; and diluting the crude neutral phytase by 10 folds, and then measuring enzyme activity of fermentation liquor, wherein, the carbon source of the enzyme-producing culture medium is wheat bran, and the nitrogen source of the enzyme-producing culture medium comprises peptone from casein and (NH4)2SO4. The neutral phytase produced by the bacillus subtilis has high enzyme activity (pH=7.0), so the neutral phytase can be applied to development of fish feed for lowering phosphorus pollution in water, and can be also applied to development of biological bacterial fertilizer for effectively utilizing organic phosphorus in soil, reducing application amount of phosphate fertilizer, improving soil microenvironment and enhancing the quality of agricultural products.

The invention discloses a fermentation medium and a method for producing plasmid DNA vaccine pSVK-CAVA for the treatment of melanoma by the fermentation medium. The high-yield fermentation medium per 1L comprises 12g of casein peptone, 16.61g of yeast extract, 3.05mL of glycerol, 1g of NH4Cl, 100mL of a mixed solution of 0.17mol / L KH2PO4 and 0.72mol / L K2HPO4, 0.185g of MgSO4 and 1mL of trace elements. The plasmid with a high volumetric yield can be obtained by an engineered bacteria E. coli XL10-Gold / CAVA working seed and the fermentation medium and the fermentation production of DNA vaccine pSVK-CAVA for the treatment of melanoma can be achieved.

The invention relates to fruit fly food source attractant. The fruit fly food source attractant is characterized in that the essential component of the attractant adopts bananas; the mass percent of the bananas to the attractant is 80 to 99 percent; preferably, the mass ratio of bananas to yeast to casein peptone is 8-10:0.01-0.03:0.9-1.1; bananas, yeast and casein peptone are mixed according to the mass ratio of 8-10:0.01-0.03:0.9-1.1, appropriate water is added to the bananas, yeast and casein, then soft material is obtained after stirring, sifting pelletization is performed so as to ensure that the granular sizes are uniform, the granular size reaches 16 to 18 meshes, the granules are pressed into flake through a pelleting press, and then finished products are obtained after drying through an oven. The fruit fly food source attractant has the characteristics that the attractant is safe and reliable to use, nonhazardous and pollution-free, and has a good attractive effect on fruit flies, and the persistent period of the attractive effect is long, and the fruit fly food source attractant is particularly applicable to strategies of fly prevention of Chinese bayberry.

The invention discloses a preparation method and a special culture medium of veterinary D-type clostridium perfringen toxin. The culture medium per 100ml comprises the following components: 1-1.5g soy peptone, 1-1.5g casein peptone, 0.5-0.75g yeast extract, 0.5-0.75g Na2HPO4.12H2O, 1-1.5g dextrin and the balance of water, wherein a pH (potential of hydrogen) value of the culture medium is 8.0-8.5. The preparation method of the D-type clostridium perfringen toxin comprises the steps of inoculating a D-type clostridium perfringen production strain into the culture medium, collecting a culture material, performing centrifugation, and then filtering a supernate. With the adoption of the method, the maximum toxicity can be improved to be 45 times a seedling standard in Regulations on National Veterinary Biological Products, and an output-input ratio can be increased to be 30-225 times that of the original traditional technology. In addition, corresponding serum neutralization titer of a toxoid vaccine prepared by the D-type clostridium perfringen toxin on a rabbit and a sheep is also improved to be 8.3 and 13.3 times a regulation standard respectively.