8 results about "Casein peptone" patented technology

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.

The invention discloses a culture medium for A type clostridium perfringens and C type clostridium perfringens, a fermentation culture method and application of fermentation toxin production. Wherein the culture medium comprises the following components: tryptone, # imgabs0 # peptone, casein peptone, a yeast extract, lactoalbumin hydrolysate, trihydroxymethyl aminomethane, sodium acetate and glucose. According to the present invention, through the specific formula composition, the A-type clostridium perfringens and the C-type clostridium perfringens can adapt to fermentation in the fermentation tank, and in the fermentation culture process, the toxin production ability of the A-type clostridium perfringens and the C-type clostridium perfringens can be significantly improved by controlling the stirring speed, the pH value of the fermentation liquid and the precise material supplementing manner. And the supernate obtained by fermentation is used as a raw material for preparing the vaccine, so that animals can generate high-level neutralizing antibodies aiming at the clostridium perfringens A and C type toxins after being immunized with the vaccine, the death of the animals caused by infection of the clostridium perfringens A and C type toxins is avoided, and the method has practical popularization and application values.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The invention provides a helicobacter pylori allochroic isolation medium as well as a preparation method and application thereof, and belongs to the technical field of helicobacter pylori culture. The helicobacter pylori color-changing separation culture medium is prepared from the following components in concentration: 4 to 6 g / L of bovine brain extract powder, 4 to 6 g / L of bovine heart extract powder, 10 to 15 g / L of agar, 21 to 25 g / L of casein peptone, 2.5 to 3.5 g / L of glucose, 4 to 6 g / L of sodium chloride, 2.5 to 3.5 g / L of disodium hydrogen phosphate, 0.5 to 1.5 g / L of beta-cyclodextrin, 1.5 to 2.5 g / L of urea, 0.1 to 0.3 g / L of bromothymol blue, 0.01 to 0.05 mL / L of bacteriostatic agent, 80 to 100 mL / L of calf serum and a solvent of water. The culture medium can obviously improve the separation culture success rate of the helicobacter pylori clinical strain, and has the characteristics of high efficiency, specificity, high bacterial colony activity and color change.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

The present application relates to the technical field of fermentation medium, more specifically, it relates to a high-density fermentation medium for streptococcus suis and preparation and application thereof.The medium comprises beef infusion powder 1-5 parts, tryptone 1-10 parts, pancreatic casein peptone 1-10 parts, soybean peptone 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, sodium chloride 1-10 parts, yeast extract 1-10 parts and glucose 1-5 parts.

The invention discloses a preparation method of casein peptone for promoting growth of bifidobacterium longum and improving freeze-drying survival, and belongs to the technical field of microorganisms, and the preparation method comprises the following steps: sequentially hydrolyzing casein by using alkaline protease, chymotrypsin and flavourzyme under different conditions, collecting peptide liquid after passing through a ceramic membrane and ultrafiltration, and drying to obtain the casein peptone for promoting growth of bifidobacterium longum. The target product casein peptone CACF is prepared after freeze drying, the peptone is used as the sole nitrogen source to culture bifidobacterium longum, the growth of the bifidobacterium longum can be promoted, the viable count of each gram of dry bacterial powder is increased, and the method has a huge application prospect.

The present application relates to blood culture bottle technical field, especially to composition and its application in the preparation of microbial culture medium, the present application combines yeast extract powder, soybean peptone and pancreatic casein peptone into nitrogen source, forming aerobic and anaerobic culture medium for clinical pathogenic microorganism culture, through comparison and verification, the aerobic and anaerobic culture medium added with the composition as described above is superior to prior art and other formula in culture speed and biomass accumulation in the growth experiment of 9 aerobic bacteria and 7 anaerobic bacteria, which shows that the composition as described above can effectively promote the performance of blood culture product, improve the ability of clinical blood stream infection detection, curb antibiotic abuse and save patients' lives.