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54 results about "Casein peptone" patented technology
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Synonym: Casein-peptone Dextrose Yeast Agar according to Buchbinder et al., Standard Methods Agar according to Buchbinder et al., Tryptone Glucose Yeast Extract Agar according to Buchbinder et al.
Fruit fly food source attractant and preparation method thereof
The invention relates to fruit fly food source attractant. The fruit fly food source attractant is characterized in that the essential component of the attractant adopts bananas; the mass percent of the bananas to the attractant is 80 to 99 percent; preferably, the mass ratio of bananas to yeast to casein peptone is 8-10:0.01-0.03:0.9-1.1; bananas, yeast and casein peptone are mixed according to the mass ratio of 8-10:0.01-0.03:0.9-1.1, appropriate water is added to the bananas, yeast and casein, then soft material is obtained after stirring, sifting pelletization is performed so as to ensure that the granular sizes are uniform, the granular size reaches 16 to 18 meshes, the granules are pressed into flake through a pelleting press, and then finished products are obtained after drying through an oven. The fruit fly food source attractant has the characteristics that the attractant is safe and reliable to use, nonhazardous and pollution-free, and has a good attractive effect on fruit flies, and the persistent period of the attractive effect is long, and the fruit fly food source attractant is particularly applicable to strategies of fly prevention of Chinese bayberry.
Owner:WENZHOU MEDICAL UNIV
Microbial-capsule-based self-repairing agent for airfield pavement, and preparation and application thereof
ActiveCN110436816APreventing Extensive CorruptionImprove repair efficiencyEpoxyCalcium carbonate precipitation
The invention provides a microbial-capsule-based self-repairing agent for an airfield pavement, and preparation and application thereof, belonging to the technical field of self-repairing agents for cracks. The self-repairing agent of the invention comprises dried spore powder, nutrients and auxiliary materials, where the nutrients are composed of urea, soy peptone, casein peptone and sodium chloride, and the auxiliary materials are composed of microcrystalline cellulose, hydroxypropyl methyl cellulose and epoxy resin E-51. The urea, the soy peptone, the casein peptone, the sodium chloride, the microcrystalline cellulose, the hydroxypropyl methyl cellulose and the dried spore powder compose core particles, and the epoxy resin E-51 wraps the core particles therein so as to form a microbialcapsule. When fine cracks of a concrete layer appear during the use of the airfield pavement, pressure will force the wall of the microbial capsule to rupture, then air and moisture will enter the capsule and activate the germination of microbial spores in the capsule, and microbial metabolism will induce the formation of a calcium carbonate precipitate, thereby realizing self-repairing of the fine cracks.
Owner:BINZHOU UNIV
Method for concreting loose sand particles through biological phosphate/carbonate composite cementing material
The invention discloses a method for concreting loose sand particles through a biological phosphate / carbonate composite cementing material. The method comprises the following steps: inoculating the second to third-generation Bacillus pasteurii strains into a soy peptone and casein peptone culture medium, thereby obtaining the third to fourth-generation Bacillus pasteurii bacterium solution; completely dissolving K2HPO4 solids in the Bacillus pasteurii bacterium solution, thereby forming phosphate bacterium solution; preparing a mixed solution of 1mol / l of urea and 2mol / l of anhydrous MgCl2; preparing two grades of quartz sand of which the particle sizes are 150microns or below and 150-300 microns respectively according to a Fuller closest packing method, and filing the quartz sand in a mold provided with a buffer pad and filter sand; respectively injecting the phosphate bacterium solution and the mixed solution into the prepared quartz sand mold according to a volume ratio of 1:1 by virtue of a peristaltic pump until the phosphate bacterium solution and the mixed solution cannot be injected; and maintaining the sample and the mold in a drying oven together for 10-15 days, performing form stripping, thereby obtaining corresponding quartz sand columns.
Owner:SOUTHEAST UNIV
Bifidobacterium bifidum and application thereof
ActiveCN108707565ACan regulate immune responseEnhanced inhibitory effectBacteriaLactobacillusEscherichia coliMortality rate
Owner:BEIJING BOJINYUAN BIOTECH
Veterinary D-type clostridium perfringen toxin, and preparation method and special culture medium of D-type clostridium perfringen toxin
ActiveCN107299070AStrong toxin production abilityToxic performance is stableAntibacterial agentsBacterial antigen ingredientsSerum neutralizationBiologic Products
The invention discloses a preparation method and a special culture medium of veterinary D-type clostridium perfringen toxin. The culture medium per 100ml comprises the following components: 1-1.5g soy peptone, 1-1.5g casein peptone, 0.5-0.75g yeast extract, 0.5-0.75g Na2HPO4.12H2O, 1-1.5g dextrin and the balance of water, wherein a pH (potential of hydrogen) value of the culture medium is 8.0-8.5. The preparation method of the D-type clostridium perfringen toxin comprises the steps of inoculating a D-type clostridium perfringen production strain into the culture medium, collecting a culture material, performing centrifugation, and then filtering a supernate. With the adoption of the method, the maximum toxicity can be improved to be 45 times a seedling standard in Regulations on National Veterinary Biological Products, and an output-input ratio can be increased to be 30-225 times that of the original traditional technology. In addition, corresponding serum neutralization titer of a toxoid vaccine prepared by the D-type clostridium perfringen toxin on a rabbit and a sheep is also improved to be 8.3 and 13.3 times a regulation standard respectively.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Microbial culture medium and use thereof
InactiveCN1746289AQuality assuranceColony morphology is goodBacteriaMicrobiology processesBiotechnologyFacultative anaerobic organism
A microbial medium and its use are disclosed. The medium consists of pancreatic casein peptolysis 5-30 proportion, yeast extractive powder 0.5-20 proportion, grape sugar 0.5-20 proportion, sodium chloride 1-15 proportion, L-cystine 0.1-5 proportion and sulfur sodium ethylate or sulfur glycolic acid 0.1-8 proportion. It is characterized by adding agar into medium with content=50-500g / cm2. It can be used for CFU count of anaerobe or facultative anaerobe.
Owner:NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
Culture medium for improving freezing tolerance of Lactobacillus bulgaricus and application of culture medium
ActiveCN108018211AImprove frost resistanceIncrease the number of live bacteria in bacteria powderMicroorganism based processesMicroorganism preservationFreeze-dryingManganese
The invention provides a culture medium for improving the freezing tolerance of Lactobacillus bulgaricus and application of the culture medium. The culture medium contains the following components byweight: 12g-30g of mannose, 5g-10g of mycose, 3g-6g of casein peptone, 3g-6g of anhydrous sodium acetate, 5g-10g of beef extract powder, 3g-6g of potassium acetate, 1.5g-4.0g of anhydrous calcium chloride, 0.1g-0.8g of magnesium sulfate, 0.1g-0.5g of manganese sulfate, 0.1g-1g of L-cysteine hydrochloride, 0.6mL-1.2mL of Tween-80 and 1000mL of distilled water. By utilizing the freezing-resistant culture medium and particularly a freeze-drying protection method provided by the invention, relatively high freeze-drying survival rate of Lactobacillus bulgaricus can be realized, the preservation period is relatively long, and the survival rate is unlikely to be decreased.
Owner:内蒙古双奇药业股份有限公司
Alkaline kation feed immunopotentiator solution, production method and uses thereof
InactiveCN101326963ARealize continuous fermentationImprove fermentation yieldBacteriaAnimal feeding stuffSodium zincateSulfite salt
The invention relates to feed additive solution which improves the immunity of alkaline cation. The additive solution of the invention is in light yellow color, with 1.38 to 1.42 of proportion and 52.0 to 182.0 of viscosity. During preparation, the target product of the invention is obtained through steps, such as high-pressure sterilization, cooling, inoculation, etc. with tryptone and casein peptone, glucose, sodium chloride, fluagel, sodium zincate, oligomeric chitosan, sodium sulfite and biotite powder. One purpose of the invention is to add immunity intensifier solution into feed to be fermented and dried, and then add the solution into the feed to feed livestock; another purpose is to add the immunity intensifier solution into the feed to directly feed the livestock; and the other purpose is to dilute the immunity intensifier solution and then directly feed the solution to the livestock as drinking water agent. The feed additive solution of the invention has the advantages that the solution can not only continuously ferment so that the whole production process is channelized and reduces the pollution probability, but also promote the digestion and absorption of animals, and improve the obvious effect in a plurality of aspects, such as animal growth, antibacterium, antivirus, anti-stress ability, medicinal effect enhancement, etc.
Owner:BEIJING SHINEJOY SHINEYEAH TECH +1
Haemophilus paragallinarum B strain fermentation medium, preparation method thereof and application thereof
ActiveCN106190909ATo promote metabolismShorten the adaptation periodBacteriaMicroorganism based processesAdditive ingredientHAEMOPHILUS B
The invention discloses a haemophilus paragallinarum B strain fermentation medium, a preparation method thereof and application thereof, and belongs to the field of agricultural microorganism. The haemophilus paragallinarum B strain fermentation medium is prepared from 5 to 100 g / L of casein peptone, 0.5 to 80 g / L of yeast powder, 0.5 to 80 g / L of polyvalent peptone, 0.5 to 20 g / L of sodium chloride, 0.5 to 20 g / L of sodium glutamate, 0.5 to 30 g / L of glucose, 0.5 to 30 g / L of fish meal peptone, 5 to 100 mL / L of chicken serum, 0.5 to 50 mL / L of microelement solution and 1 to 30 mL / L of NAD (Nicotinamide Adenine Dinucleotide) solution. The haemophilus paragallinarum B strain fermentation medium disclosed by the invention contains all nutritional ingredients which are needed for growth of haemophilus paragallinarum B strains, growth and metabolism of the strains are facilitated, the adaption period of thallus is shortened, the adaptive capacity of the thallus after inoculation is strong, the fermentation period is short, the viable bacteria quantity during harvesting is high, and the haemophilus paragallinarum B strain fermentation medium is suitable for large-scale application and has a wide prospect.
Owner:山东滨州沃华生物工程有限公司
Culture medium for preparing epothilone through fermenting myxococcus Xanthus for heterologously expressing epothilone gene
ActiveCN105200090AIncrease productionRaise the level of fermentationMicroorganism based processesFermentationHeterologousPotassium
The invention discloses a culture medium for preparing epothilone through fermenting myxococcus Xanthus for heterologously expressing an epothilone gene. The culture medium comprises the following components with the contents: 10g / L of casein peptone, 1.0g / L of MgSO4.7H2O, 2.07g / L of 3-(N-morpholinyl)propanesulfonic acid sodium salt, 5-15ml / L of cis-9-octadecenoic acid methyl ester, 0.8-1.6g / L of threonine, 0.5-1.2g / L of methionine, 1.0-3.0g / L of potassium propionate, 1mL / L of a microelement solution and 20mL / L of macroporous resin XAD-16. The culture medium has the remarkable characteristic that cis-9-octadecenoic acid methyl ester, L-threonine, methionine and potassium propionate are added. Experiments prove that the fermentation level and efficiency of an epothilone compound are remarkably improved through combination and optimization, and the culture medium has a favorable application prospect in production of the epothilone through industrial fermentation of myxococcus Xanthus for heterologously expressing the epothilone gene.
Owner:SHANDONG UNIV
Method for high density culture of lactobacillus fermentum La-Y1, and application thereof
InactiveCN102807959ALow costHigh bacterial countBacteriaMicroorganism based processesSodium acetatePhosphate
The invention discloses a method for high density culture of a strain of lactobacillus fermentum La-Y1. The method comprises that: (1) fermentation conditions are a culture temperature of 37 DEG C, an initial pH value of 6.0, and an inoculation amount of the lactobacillus fermentum La-Y1 of 5%; and (2) fermentation medium components comprise, by weight, 2 parts of glucose, 0.5 part of beef extract, 0.5 part of casein peptone, 2 parts of corn steep powder, 0.2 part of ammonium citrate dibasic, 0.5 part of sodium acetate, 0.1 part of tween 80, 0.025 part of manganese sulfate, 0.058 part of magnesium sulfate, and 0.2 part of potassium dihydrogen phosphate. The present invention further discloses an application method of the lactobacillus fermentum La-Y1. With the present invention, the number of the lactobacillus fermentum La-Y1 in the culture medium of the present invention is relatively increased by more than 10 times compared with the number of the lactobacillus fermentum La-Y1 in a basic MRS culture medium, wherein the bacterial concentration is 3.05*10<10> cfu / mL. According to the present invention, beef extract content and casein peptone content in the culture medium of the present invention are only 0.5% so as to substantially reduce cost compared to the basic MRS culture medium, and realistic significances are provided for development of direct vat set starters.
Owner:绍兴市古煌酿酒有限公司
Preparation method for anti-oxidation lactobacillus casei fermented goat milk
InactiveCN107771940AImproves antioxidant activityPromote proliferationMilk preparationDPPHSheep milk
The invention discloses a preparation method for anti-oxidation lactobacillus casei fermented goat milk. The preparation method comprises the following steps: preparing the recovery goat milk by degreased goat milk powder and distilled water; inoculating lactobacillus casei strains in the recovery goat milk, and adding honey tree polysaccharide, calcium lactate, casein peptone, xylitol and whey isolate protein, and performing the anaerobic culture to obtain the fermented goat milk; and after-ripening the fermented goat milk to obtain the anti-oxidation lactobacillus casei fermented goat milk.The added calcium lactate and casein peptone are good for producing the antioxidative peptide capacity by the lactobacillus casei fermented goat milk, the antioxidant activity of the fermented goat milk is improved, mass multiplication of the lactobacillus casei can be promoted, and the fermented goat milk is beneficial to regulate the intestinal flora balance and the immunity of the organism. Theadded honey tree polysaccharide has the functions of a stabilizer and reducing blood glucose, and is capable of increasing the functionality of the fermented goat milk. The viable count of the lactobacillus casei in the prepared anti-oxidation lactobacillus casei fermented goat milk is more than 109 cfu / mL. The DPPH free radical scavenging rate and hydroxyl free radical scavenging rate of the fermented goat milk are 55%-75% and 70%-90%.
Owner:SHAANXI UNIV OF SCI & TECH
Melanoma therapeutic plasmid DNA (deoxyribonucleic acid) vaccine pSVK-CAVA preparation method as well as dedicated engineering bacterium and fermentation culture medium thereof
The invention discloses a melanoma therapeutic plasmid DNA (deoxyribonucleic acid) vaccine pSVK-CAVA preparation method and a dedicated engineering bacterium and a fermentation culture medium thereof. The engineering bacterium is Escherichia coli XL10-Gold which contains converted melanoma therapeutic plasmid DNA pSVK-CAVA and is named as E.coli XL10-Gold / CAVA. Each 1L of high-yield fermentation culture medium comprises: 12g of casein peptone, 16.61g of yeast extract, 3.05mL of glycerol, 1g of NH4Cl, 100mL of mixed solution of 0.17mol / L of KH2PO4 and 0.72mol / L of K2HPO4, 0.185g of MgSO4, and 1mL of trace elements. The engineering bacterium is high in stability, can be subjected to continuous passage for more than 30 times, can meet the demand of plasmid DNA vaccine pSVK-CAVA pilot test process, the plasmid volume yield obtained after fermentation of the high-yield culture medium is high, and the engineering bacterium can be used for fermentation production of the melanoma therapeutic plasmid DNA vaccine pSVK-CAVA.
Owner:INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
Method for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum
InactiveCN111909977AOptimize mass ratioIncrease the amount of controlBacteriaMicroorganism based processesBiotechnologyActive agent
The invention discloses a fermentation condition for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum CGMCC5297. An optimized fermentation culture medium formula comprises 10-60g / L of glucose, 8-12g / L of casein peptone and 3-7.5 g / L of yeast powder, and the optimized fermentation condition of a fermentation tank is as follows: the ventilation ratio is 4.7-6.4 vvm, the rotating speed is 100-150r / min, and the inoculum size is 1.5-3.5%. The pH value of the culture medium is 5.8-6.2. According to the method, the mass ratio of a carbon source to a nitrogen source in the culture medium is optimized, besides, the addition amount of inorganic salt and the addition amount of a surfactant are controlled, the yield of the antibacterial peptide is greatly increased, the titer of the obtained antibacterial peptide is 1.4 times that of the antibacterial peptide before being optimized, and good industrial production potential is shown.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Staphylococcus aureus chromogenic culture media
InactiveCN104195217AEasy to operateLow costMicrobiological testing/measurementDipotassium hydrogen phosphateSucrose
The invention belongs to the field of microorganisms and in particular relates to a staphylococcus aureus chromogenic culture media. The culture media comprises the following components: a trisaccharide iron (TSI) agar culture media, 12 g / L casein peptone, 6 g / L mannitol, 2.0 g / L dipotassium hydrogen phosphate, 0.5 g / L potassium dihydrogen phosphate, 8 g / L glycine, 12 g / L sodium pyruvate and a 0.16 g / L color developing agent, wherein the pH value is 7.2-7.4. According to the staphylococcus aureus chromogenic culture media, 1 L of the TSI agar culture media contains 15.0 g of tryptone, 10.0 g of soybean peptone, 5.0 g of yeast powder, 4.0 g of sodium chloride, 10.0 g of sucrose, 0.2 g of ferrous sulfate, 0.3 g of sodium thiosulfate, 15.0 g of agar, 0.04 g of bromcresol purple and the balance of distilled water. The staphylococcus aureus chromogenic culture media is simple in component, convenient to prepare, low in cost and favorable for popularization and application; staphylococcus aurei are high in growth speed, low in aberration rate and convenient to observe and analyze.
Owner:QINGDAO RUNXIN WEIYE TECH & TRADE
Fermentation medium and method for producing plasmid DNA vaccine pSVK-CAVA for treatment of melanoma by fermentation medium
Owner:INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
Listeria monocytogenes selective medium
InactiveCN105506054AImprove separation rateReliable detectionMicrobiological testing/measurementArginineGluconic acid
The invention discloses a listeria monocytogenes selective medium, and belongs to the field of detection of microculture. The listeria monocytogenes selective medium is characterized in that the prepared 1000 ml culture medium comprises the following components: yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, zinc gluconate, sodium dihydrogen phosphate, optical, polymyxin,bacitracin, gamma-arginine hydroxy and sterile horse blood protein, and distilled water is added into the components to 1000 ml. Compared with the prior art, the selective medium has the characteristics of being reliable in detection result, and high in listeria monocytogenes separation rate.
Owner:夏淑平
Culture medium and culture method for cultivating Tetraselmis subcordiformis by utilizing winery wastewater
ActiveCN103966101AReduce production rateLow benefitUnicellular algaeMicroorganism based processesAdditive ingredientCulture fluid
The invention discloses a method for cultivating Tetraselmis subcordiformis by utilizing winery wastewater. The technique can treat wine wastewater at low cost, change wastes into valuable substances, and obtain Tetraselmis subcordiformis with important bait value, thereby implementing multiple purposes. The traditional Tetraselmis subcordiformis culture solution has fewer nutrients and uses an open cement track tank for culture, and thus, has the defects of low growth speed, low yield, high investment, high tendency to pollution and low benefit. At present, a water tower made from high-density polyethylene with the volume of 3 cubic meters, which has the advantages of low cost and low tendency to pollution, can be reutilized and is easy to operate, is adopted; and wine wastewater, urea sea mud extracting solution, soil extracting solution, cattle manure leaching solution, L-cysteine, casein peptone, medical stone powder, sodium dihydrogen phosphate, S3 vitamin, human urine and other nutritional ingredients are added into the culture medium, and the organic fertilizer and inorganic fertilizer are mixed for use, so that the nutrition is more comprehensive and balanced, thereby greatly enhancing the growth speed of the Tetraselmis subcordiformis and enhancing the yield by 350%.
Owner:江西康膳源油脂有限公司
Preparation method and application of veterinary-use A type clostridium perfringens toxins
ActiveCN109943507AThe preparation method is simple and easyLow costAntibacterial agentsBacterial antigen ingredientsClostridium perfringens toxoidBacillus perfringens
The invention discloses a preparation method and application of veterinary-use A type clostridium perfringens toxoids. The preparation method of the toxoids includes the following steps that A type clostridium perfringens production strains are inoculated into a culture medium for fermentation and culture, a fermentation product is obtained, then the fermentation product is inactivated and detoxified in L-lysine and a formaldehyde aqueous solution under the pH of 6.8, and centrifugal supernatant of the successfully inactivated and detoxified qualified bacterial solution refer to toxoids. The culture medium includes casein peptone, yeast extract powder, CaCl2, ZnSO4 7H2O, Na2HPO4 12H2O, KH2PO4, and glucose. The toxicity of the A type clostridium perfringens toxoids prepared by the method can be raised to be 12.5 times of the standard for seedling cultivation, the neutralization titer, in first and second immune serum of domestic rabbits, of the toxoids can be raised to 4 and 50 times ofa traditional process respectively and maximally, and the ratio of the serum titer to cost can be raised to 48.8 and 1216.4 times of the traditional process.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Semi-synthetic culture medium for dictyostelium discoideum and preparing method thereof
The invention discloses a preparing method of semi-synthetic medium of dish group net prosthecae, which comprises the following steps: choosing yeast powder, bacteriological tryptone, casein peptone, protein peptone and carbon source; setting amylaceum or barley sugar as carbon source; choosing KCl, MgCl2 and CaCl2 as inorganic salt; choosing KH2PO4 and Na2HPO4 as microcosmic salt cushioning liquid; allocating the other component as water; adding yeast powder, bacteriological tryptone, casein peptone, protein peptone into inorganic salt and microcosmic salt cushioning liquid; adjusting pH value with NaOH or H3PO4; sterilizing with high pressure; getting semi-synthetic medium without carbon source; allocating amylaceum or barley sugar solution; sterilizing with high pressure or degerming with film filtration; adding in to semi-synthetic medium without carbon source.
Owner:XIAMEN UNIV
Culture medium for separating fungi from yeast-like fungi and preparation method
PendingCN110607243AIncrease contentThe ratio is easy to controlFungiMicroorganism based processesCulture mediumsChemistry
The invention relates to the technical field of medical chemiluminescence immunoassay detection, and particularly relates to a culture medium for separating fungi from yeast-like fungi and a preparation method thereof. Casein peptone, animal peptone and glucose are used for providing a proper carbon source and a proper nitrogen source for microorganisms, the ratio of the whole carbon source to thewhole nitrogen source is controllable, meanwhile, the content of the glucose is higher, the pH value of the whole culture medium tends to 5.5-6.5, and growth of fungi and yeast-like fungi is facilitated. Meanwhile, single chloramphenicol is adopted as a bacterial inhibitor to effectively inhibit the growth of bacteria, and to reduce the probability that part of to-be-separated fungi are inhibiteddue to cooperation of multiple bacteriostatic agents. The invention solves the problems that: an existing culture medium for separating fungi is single in carbon source and nitrogen source component,the imbalance of the ratio of a carbon source to a nitrogen source is likely to occur, meanwhile, multiple bacteriostatic agents are combined for bacteriostasis, the bacteriostatic effect on part offungi is overlapped, and the effect of separating part of fungi is poor.
Owner:中秀科技股份有限公司
Novel listeriosis selective separating medium, preparation method and application thereof
ActiveCN107385010AGrowth inhibitionPromote growthMicrobiological testing/measurementMicroorganism based processesSodium bicarbonatePropolis
The invention relates to the technical flied of biological examination, and especially relates to a novel listeriosis selective separating medium, a preparation method and an application thereof. A formula of the novel listeriosis selective separating medium comprises 4 g of beef brain infusion powder, 4 g of beef heart infusion powder, 5 g of peptone, 16 g of casein peptone, 5 g of sodium chloride, 2 g of sodium pyruvate, 2 g of glucose, 2.5 g of sodium bicarbonate, 1 g of phosphoglyceride, 12. 5 g of agar powder, 5 ml of a phosphatidylinositol solution with concentration being 0.1 g / ml, 5 ml of a pyrans glucoside solution with concentration being 0.02 g / ml, and 5 ml of a propolis solution with concentration being 0.0125-128.0 [mu]g / ml in each liter of distilled water. The method employs natural antibiosis substance propolis as a selective additive of the listeriosis selective separating medium, an improved basic medium is combined for usage, the background microbe growth in various food can be effectively inhibited, so that the growth of the listeriosis in the selective medium is good, the growth of 13 types of serotype bacterial strains of listeriosis is good, and the pathogenic bacteria can be accurately and sensitively detected in the various food.
Owner:HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
Preparation method of avibacterium paragallinarum culture medium
PendingCN110747148AThe cultivation method is simpleShorten the growth cycleBacteriaMicroorganism based processesBiotechnologyMonosodium glutamate
The present invention provides a formula of an avibacterium paragallinarum culture medium. The formula comprises the following raw materials: 0.5% of polypeptone, 0.5% of casein peptone, 0.5% of yeastextract powder, 0.5% of sodium chloride, 0.5% of sodium glutamate, 0.02% of a coenzyme NAD aqueous solution, 0.2% of glucose and 6% of chicken serum. The present invention also discloses a culture method established according to the formula. The method is simple, growth period is short, the number of viable bacteria of the cultured avibacterium paragallinarum is high, and moreover, the provided avibacterium paragallinarum culture medium is low in costs and easy to produce on a large scale.
Owner:TIANJIN RINGPU BIO TECH +1
Method for improving whiteness of xanthan gum through fermentation condition control and method for detecting xanthan gum fermentation broth
InactiveCN108048505AReduce extraction costsImprove qualityMicrobiological testing/measurementBiological material analysisYeastSucrose
The invention discloses a method for improving the whiteness of xanthan gum through fermentation condition control and a method for detecting a xanthan gum fermentation broth. The method for improvingthe whiteness of the xanthan gum through fermentation condition control includes the following steps of firstly, conducting slope culturing; secondly, conducting liquid fermentation culturing, wherein the liquid fermentation culturing includes the substeps of inoculating a ring slope strain in an NF-5 liquid fermentation culture medium and conducting culturing for 80-100 h under the aerobic conditions at the culture temperature of 30 DEG C, and the fermentation culture medium comprises 35 g / L cane sugar, 0.5-1 g / L yeast powder, 0.5-1 g / L casein peptone, 0.5-1 g / L uracil, 0.5-1 g / L (NH4)HPO4,1 g / L CaCl2 and 1 g / L KH2PO4. The method includes simple steps and is low in product extraction cost and good in product quality.
Owner:TAIZHOU POLYTECHNIC COLLEGE
Veterinary clostidrium novyi exotoxin, as well as preparation method, toxin production culture medium and application
ActiveCN110904007AOptimizing Toxigenic Culture ConditionsSkip the digestion processBacteriaFood processingZoologyCasein peptone
The invention discloses a veterinary clostidrium novyi exotoxin, a preparation method thereof, a toxin production culture medium, and applications. Every 1000 ml of the toxin production culture mediumis prepared from the following raw materials in parts by weight: 15-25 g of peptone, 5-10 g of tryptone, 5-10 g of casein peptone, 1-3 g of yeast extract powder, 1-3 g of NaCl, 5-10 g of KH2PO4, 5-10g of dextrin or maltose, 95-105 g of rust-free iron nails or scrap iron, and the balance water for injection. The highest toxicity of a veterinary clostidrium novyi exotoxin solution prepared by themethod can reach 1MLD <= 0.00008 ml, and the toxicity is 6.25 times higher than that in a standard. In addition, the neutralizing titer of sheep black plague and bradsot combined inactivated vaccine prepared from the veterinary clostidrium novyi exotoxin in corresponding serum of rabbits and sheep also reaches or is higher than the standard of related regulations.
Owner:JINYUBAOLING BIO PHARMA CO LTD
Medium for the isolation and enumeration of Clostridium butyricum
ActiveCN111763652BAchieve separationGrowth inhibitionBacteriaMicrobiological testing/measurementBacteroidesBromothymol blue
The invention relates to a culture medium for isolating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Including tryptone 15.0g, beef extract powder 3.0g, yeast extract powder 1.0g, sodium chloride 5.0g, L-cysteine hydrochloride 0.5g, sodium thioglycolate 0.5g, xylose 10.0g, Bromothymol blue 0.04g, agar powder 15.0g, purified water 900ml, D-cycloserine 0.25g, kanamycin sulfate 0.012g, egg yolk 25ml. Compared with the prior art, the beneficial effects of the present invention are: first, the culture medium of the present invention can make Clostridium butyricum It can be clearly distinguished from other bacteria by visual observation, thereby achieving the purpose of isolating and counting Clostridium butyricum. Second, the effect is remarkable, direct and effective, and low in cost.
Owner:山东拓普生物工程有限公司
Listerella selective culture medium
InactiveCN105543326AImprove separation rateReliable detectionMicrobiological testing/measurementMicroorganism based processesBiotechnologyArginine
The invention discloses a Listerella selective culture medium, belonging to the field of inspection microbe growth. The invention is characterized in that every 1000ml of culture medium contains yeast leaching powder, beef powder, casein peptone, agar, glucose, sodium chloride, yeast zinc, sodium dihydrogen phosphate, gamma-hydroxy arginine, N-acetyl-D-glucose amine, glutathione, bacitracin, sterile horse blood protein and the balance of distilled water. Compared with the prior art, the culture medium disclosed by the invention has the characteristics of high detection reliability and high separation rate of Listerella.
Owner:刘名霞
Fruit fly food source attractant and preparation method thereof
The invention relates to fruit fly food source attractant. The fruit fly food source attractant is characterized in that the essential component of the attractant adopts bananas; the mass percent of the bananas to the attractant is 80 to 99 percent; preferably, the mass ratio of bananas to yeast to casein peptone is 8-10:0.01-0.03:0.9-1.1; bananas, yeast and casein peptone are mixed according to the mass ratio of 8-10:0.01-0.03:0.9-1.1, appropriate water is added to the bananas, yeast and casein, then soft material is obtained after stirring, sifting pelletization is performed so as to ensure that the granular sizes are uniform, the granular size reaches 16 to 18 meshes, the granules are pressed into flake through a pelleting press, and then finished products are obtained after drying through an oven. The fruit fly food source attractant has the characteristics that the attractant is safe and reliable to use, nonhazardous and pollution-free, and has a good attractive effect on fruit flies, and the persistent period of the attractive effect is long, and the fruit fly food source attractant is particularly applicable to strategies of fly prevention of Chinese bayberry.
Owner:WENZHOU MEDICAL UNIV
Clostridium perfringens type d toxin for veterinary use and its preparation method and special medium
ActiveCN107299070BGood repeatabilityStrong toxin production abilityAntibacterial agentsBacterial antigen ingredientsClostridium perfringens toxoidBacillus perfringens
The invention discloses a preparation method and a special culture medium of veterinary D-type clostridium perfringen toxin. The culture medium per 100ml comprises the following components: 1-1.5g soy peptone, 1-1.5g casein peptone, 0.5-0.75g yeast extract, 0.5-0.75g Na2HPO4.12H2O, 1-1.5g dextrin and the balance of water, wherein a pH (potential of hydrogen) value of the culture medium is 8.0-8.5. The preparation method of the D-type clostridium perfringen toxin comprises the steps of inoculating a D-type clostridium perfringen production strain into the culture medium, collecting a culture material, performing centrifugation, and then filtering a supernate. With the adoption of the method, the maximum toxicity can be improved to be 45 times a seedling standard in Regulations on National Veterinary Biological Products, and an output-input ratio can be increased to be 30-225 times that of the original traditional technology. In addition, corresponding serum neutralization titer of a toxoid vaccine prepared by the D-type clostridium perfringen toxin on a rabbit and a sheep is also improved to be 8.3 and 13.3 times a regulation standard respectively.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
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