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Diagnostic kit for Newcastle disease virus antibody

A diagnostic kit and technology of chicken Newcastle disease virus, applied in the field of diagnosis of animal infectious diseases, can solve the problems of lack of immune antibody monitoring conditions, inability to adjust immunization procedures, irrational immunization procedures, etc.

Inactive Publication Date: 2011-03-30
河南后羿生物工程股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunization has become the most important means of controlling Newcastle disease, but Newcastle disease still occurs frequently in immunized chicken flocks. The main reason is that the immunization procedure is unreasonable, the lack of immune antibody monitoring conditions, and the inability to timely adjust the immunization according to the epidemic situation and the characteristics of the epidemic area. program

Method used

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Experimental program
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Embodiment Construction

[0017] The concrete composition of kit of the present invention is as follows:

[0018] 1 Material:

[0019] 1.1 Reference serum The standard positive serum is 6-week-old chicks immunized with LaSota vaccine strain, blood was collected 2 weeks later to separate the serum, and the HI titer was determined to be 1:1024-1:2048 by HI test, and stored at -80°C after aliquoting. Negative sera were chicken sera that had never been vaccinated and had antibody levels close to the average value of negative sera.

[0020] 1.2 The ELISA reaction plate is the product of Nunc Company in Denmark, and the rabbit anti-chicken enzyme-labeled antibody and the substrate OPD are the products of Sigma Company.

[0021] 1.3 Experimental animals SPF chickens and chicken embryos are all products of Merial Company.

[0022] 2 Antigen preparation:

[0023] 2.1 Rejuvenation and proliferation of the virus Dilute the Lasota vaccine strain 100 times, inoculate in 9-11 days old SPF chicken embryos for reju...

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Abstract

The invention relates to a diagnostic kit for a Newcastle disease virus antibody, which comprises 10-time concentrated washing liquid, serum diluent, standard serum, an anti-chicken immunoglobulin G (IgG) enzyme-labeled antibody, enzyme-labeled antibody diluent, a color development agent A, a color development agent B and stop solution. In an enzyme-linked immunosorbent assay (ELISA) diagnostic kit for detecting the Newcastle disease virus antibody, the established method has specificity, sensitivity and operability. In the aspect of sensitivity tests, the coincidence rate of the established method and a method of immunofluorescence technology is 86 percent, and compared with the method of the immunofluorescence technology, the established method is more sensitive and has the zero relevance ratio in other causes of disease pathogeny; and compared with hemagglutination inhibition (HI), the method has the advantages of quickness and low cost, and can be used for diagnosing and monitoring Newcastle disease virus, and the result is easy to determine.

Description

technical field [0001] The invention relates to a diagnostic kit for chicken Newcastle disease virus antibody, which belongs to the technical field of diagnosis of animal infectious diseases. Background technique [0002] Newcastle disease (Newcastle Disease, ND) is an acute, highly contagious infectious disease of chickens caused by Newcastle disease virus of the paramyxovirus family (paramyxovirus), often showing a septic process. The main features are dyspnea, diarrhea, nervous disturbance, and mucosal and serosal hemorrhages. The disease spreads quickly and has a high mortality rate, and is listed as the first serious infectious disease. [0003] Looking back at history, Newcastle disease has caused huge economic losses in the world. It is generally believed that Newcastle disease first broke out in Java in Indonesia and Newcastle Everton in the United Kingdom in 1926. Newcastle disease was first reported in Henan, my country in 1935. In the 1950s, the disease was wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
Inventor 吴红云范金红李建正
Owner 河南后羿生物工程股份有限公司
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