Group I type 4 aviadenovirus strain WZ and application of strain WZ

A technology of poultry adenoviruses and strains, applied in the direction of viruses, antiviral agents, virus antigen components, etc., can solve the problems of no related products, achieve good specificity, prevent poultry inclusion body hepatitis, and good immunogenicity

Active Publication Date: 2017-05-31
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, there is currently no commercially available related product

Method used

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  • Group I type 4 aviadenovirus strain WZ and application of strain WZ
  • Group I type 4 aviadenovirus strain WZ and application of strain WZ
  • Group I type 4 aviadenovirus strain WZ and application of strain WZ

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Experimental program
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Effect test

preparation example Construction

[0038] The preparation method of the above-mentioned virus solution comprising the avian adenovirus group I group 4 type 4 strain WZ strain according to claim 1, comprising the following steps: passing chicken embryos of the avian adenovirus group I group 4 type 4 virus strain WZ strain Inoculate SPF chickens with the virus, harvest the liver tissue of infected dead chickens, grind, freeze-thaw, and centrifuge, and the supernatant obtained is the virus liquid; or inoculate chicken embryo liver cells for culture, and harvest the cell supernatant after freezing and thawing. virus fluid.

[0039] Application of the above-mentioned avian adenovirus group I type 4 strain WZ strain in the preparation of group I group 4 avian adenovirus vaccine.

[0040] A poultry adenovirus group I type 4 virus vaccine, the active ingredient of the vaccine comprises the inactivated poultry adenovirus group I type 4 virus strain according to claim 1.

[0041]As in the above-mentioned preparation met...

Embodiment 1

[0045] Application of the above-mentioned avian adenovirus group I type 4 strain WZ strain in the preparation of egg yolk antibody and antiserum for virus treatment. Example 1: Isolation and identification of group I group 4 avian adenovirus strain WZ strain

[0046] Since 2014, poultry inclusion body hepatitis and pericardial effusion syndrome infectious diseases have broken out in chicken flocks in many chicken raising areas in my country, with morbidity and mortality as high as 30%-70%, which has caused serious economic damage to the chicken industry. loss. The pathological changes at autopsy are mainly hepatitis, the liver is light in color, brittle, and enlarged, and there are hemorrhage points in the liver and skeletal muscle. Microscopic examination shows that there are large, round or irregular basophilic or eosinophilic nuclei in the liver cells. Body; pericardium all have varying degrees of effusion, and the effusion is light yellow or grass green, or jelly-like. Af...

Embodiment 2

[0077] Embodiment 2: Preparation of virus seed of group I group 4 avian adenovirus WZ strain

[0078] The FAdV 4 WZ strain virus strain that has been plaque-purified was expanded and cultured on chicken embryo liver cells by cell culture method, and the CCID of the WZ strain was determined at the same time 50 , the virus liquid obtained by cell culture at this time is the cell culture virus liquid. The cell culture virus liquid was prepared according to 10 6 .0 CCID 50 Intramuscularly inoculate 50-day-old SPF chickens with the same dose, harvest the liver tissue of the dead chickens, grind them, and centrifuge the supernatant to determine the virus content. The supernatant is the virus liquid used to prepare the vaccine. Will test for sterility and virus content ≥10 -7.5 CCID 50 The virus liquid was quantitatively subpackaged, freeze-dried and stored, and the original seed batch was established.

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Abstract

The invention discloses a group I type 4 aviadenovirus strain WZ. The strain has a preservation number of China Center for Type Culture Collection (CCTCC) No: V201662, is named as group I type 4 aviadenovirus strain WZ in a classified way, and is preserved in December 14, 2016; a group I type 4 viral vaccine of the aviadenovirus is prepared from the inactivated group I type 4 aviadenovirus strain which is taken as an active ingredient; the invention also provides application of the group I type 4 aviadenovirus strain WZ in preparation of an agar gel precipitating antigen, a hemagglutination inhibition (HI) antigen and positive serum antigen reagent which are used for diagnosing the group I type 4 virus of the aviadenovirus, and preparation of egg yolk antibody and antiserum which are used for treating the group I type 4 virus of the aviadenovirus. After the vaccine prepared by using the group I type 4 aviadenovirus strain is used for immunizing, the toxin attacking protection rate for the group I type 4 aviadenovirus strain WZ reaches 90-100%. As a vaccine strain with good manufacturing effect, the group I type 4 aviadenovirus strain WZ can be used for preventing avian inclusion body hepatitis and pericardial effusion syndrome, and also can be used for virus identification and epidemiological investigation.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an avian adenovirus group I type 4 strain WZ strain and application thereof. Background technique [0002] Avian adenoviruses can be divided into three groups according to their antigenic properties, group I, group II and group III, and there are significant differences in antigenicity among different groups. Group I adenoviruses are widely distributed in poultry farms and have a common group-specific antigen; they can be isolated from chickens, ducks, geese, turkeys, etc. There are 12 group I adenoviruses that can infect chickens serotype. Group II adenoviruses include turkey hemorrhagic enteritis virus, pheasant marble spleen disease virus, and chicken large spleen disease virus. Group III avian adenoviruses contain only egg drop syndrome viruses. Among group I avian adenoviruses, serotype 4 (FAdV 4) can cause inclusion body hepatitis and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/235A61P31/20A61K47/26A61K39/295A61K39/17A61K39/215A61K39/145A61P31/14A61P31/16G01N33/569C12R1/93
CPCA61K39/12A61K47/26A61K2039/5252A61K2039/552A61K2039/70C12N7/00C12N2710/10221C12N2710/10234C12N2710/10251C12N2760/16134C12N2760/18134C12N2770/20034G01N33/56983
Inventor 李新生黄宗梅崔保安周云飞周薇帆赵玉杰王洁琼高文明张剑刘琳陈盼盼王莉
Owner HENAN AGRICULTURAL UNIVERSITY
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