The application discloses a kind of target sequencing primer set and kit for detecting multiple
rodent carrying pathogenic microorganisms.The
nucleotide sequence of the primer set is shown as SEQ ID NO:1-400.The kit comprises the primer set shown as SEQ ID NO:1-400.The application can simultaneously detect 50 kinds of
rodent carrying pathogenic microorganisms, with the advantages of high detection
throughput, clear
pathogen spectrum range, low sequencing cost, high sensitivity, and no influence of host
nucleic acid, etc.The target sequencing primer set and kit are developed for common
pathogen spectrum carried by rodents, which can effectively make up for the shortcomings of prior art, expand the detection range, improve the detection sensitivity, and provide key
technical support for early warning, prevention and control of plague, hemorrhagic fever with renal syndrome, fever with thrombocytopenia syndrome and other
rodent-borne diseases.