14 results about "Hemorrhagic Fevers" patented technology

The invention belongs to the technical field of construction methods of disease animal models, and particularly relates to a construction method and application of a renal syndrome hemorrhagic fever mouse model. According to the HTNV infected mouse model established by the method disclosed by the invention, the symptom of the experimental mouse is close to the clinical symptom of a severe hemorrhagic fever with renal syndrome after challenge, the complete lethality and repeatability are high, and the HTNV infected mouse model has the advantages of simple operation, low cost and high efficiency. The method can be used for research on the HTNV pathogenic mechanism and immune mechanism and research and development of antiviral drugs and vaccines, and has great application value for perfecting an HTNV full-chain prevention and control system.

The invention relates to the technical field of pathogen detection, in particular to a hemorrhagic fever multi-pathogen nucleic acid joint detection lateral chromatography method which comprises the following steps: performing sequence alignment, target gene determination and primer screening based on hantavirus or dengue virus; an RT-PCR detection system is constructed; adding a reaction product of an RT-PCR detection system into the diluent, fully blowing, sucking and uniformly mixing, and then completely dropwise adding into a sample hole of the FAM immunochromatography test strip for amplification, so as to obtain an amplification product; the detection result is positive, only the quality control line emits the red light or the quality control line does not emit the light, the detection is invalid, the method is simple and convenient to operate, the process from sample treatment to result interpretation can be completed within one hour, and the detection is rapid. The problems that a traditional detection method is long in detection time, and multiple pathogens cannot be identified at the same time are solved.

This invention relates to the field of vaccine preparation technology, specifically disclosing a VSV-based HTNV vaccine, its preparation method, and its application. The method involves constructing a recombinant plasmid by inserting the nucleotide sequence of the HTNV envelope glycoprotein into the recombinant VSV vector plasmid, followed by packaging and processing to obtain an immunogenic HTNV recombinant VSV vaccine. The HTNV vaccine prepared by this invention provides a novel candidate vaccine for the prevention and treatment of hemorrhagic fever with renal syndrome.

The embodiments disclosed herein utilize RNA targeting effectors to provide a robust CRISPR-based diagnostic for hemorrhagic fever virus applications. Embodiments disclosed herein can differentiate between hemorrhagic fever viruses that present with similar symptoms, as well as between strains of a hemorrhagic fever virus.

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus that causes severe hemorrhagic fever disease in humans. Currently, no licensed CCHF vaccines exist, and the protective epitopes remain unclear. Here, we tested a DNA vaccine expressing the M-segment glycoprotein precursor gene (GPC) of the laboratory CCHFV strain CCHFV-IbAr 10200 (CCHFV-M10200). Increasing the dose of CCHFV-M 10200 provides complete protection from homologous CCHFV challenge in mice, and significant (80%) protection from challenge with the clinically relevant, heterologous CCHFV-Afg09-2990 strain. We also report complete protection from CCHFV-Afg09-2990 challenge following vaccination with a CCHFV-Afg09-2990 GPC expressing DNA vaccine (CCHFV-M Afgog). Finally, we show that the non-structural M-segment protein, GP38, influences CCHF vaccine immunogenicity and provides significant protection from homologous CCHFV challenge. Our results demonstrate that M-segment DNA vaccines elicit protective CCHF immunity and further illustrate the immunorelevance of GP38.

The invention relates to the technical field of biological detection, and discloses a composition for detecting hemorrhagic fever related pathogens, a kit and application. The composition comprises detection primers and probes for various serotypes of dengue fever viruses, burkholderia pseudomallei, leptospira, plasmodium noiri and other pathogens causing hemorrhagic fever diseases, the pathogens can be detected at the same time through a multiplex PCR technology, the detection speed is high, the efficiency is high, and the detection time is short. The method has the advantages of high accuracy and sensitivity.

The invention discloses a multiplex nucleic acid detection method for eight hemorrhagic fever viruses based on double-probe hybridization and application of the multiplex nucleic acid detection method. The invention designs and screens eight important virulent hemorrhagic fever viruses (Lassa fever virus, Sudan Ebola virus, Zaire Ebola virus, yellow fever virus, Marburg virus, Maautumn Bo virus, Rift Valley fever virus and Sinnocypress virus) to obtain a set of primer probe set suitable for single-tube multiplex qPCR (quantitative polymerase chain reaction) detection. Conditions such as enzyme reaction liquid, primer probe concentration, THO: PCO ratio and the like used for single-tube multiplex qPCR amplification are explored and optimized, and a sensitive and specific single-tube multiplex qPCR detection method for eight hemorrhagic fever viruses is established. Compared with the prior art, the single-tube multiple qPCR detection method has the advantages that the eight hemorrhagic fever viruses can be detected and identified through one-time amplification reaction, and compared with multiple single detection, the detection efficiency is remarkably improved, and the detection cost is remarkably reduced.

The application discloses primers, a kit and a method for detecting pathogenic bacteria of fever with hemorrhage syndrome, and nucleotide sequences of the primers are shown as SEQ ID NO. 1 to SEQ ID NO. 262. In the application, 131 pairs of specific primers are designed according to highly-conserved regions of 26 pathogenic bacteria of fever with hemorrhage related diseases, and a 26-pathogenic-bacteria detection method for fever with hemorrhage is established. The detection method can more completely cover the detection of pathogenic bacteria of fever with hemorrhage related diseases, has high accuracy, strong specificity and high sensitivity, can realize rapid detection in a short TAT (time to answer) of 15 hours, reduces mortality, and improves precise medication.

Designed polypeptide sequences are described, and their use as vaccines against pathogens which cause hemorrhagic fever, such as viruses of the Ebolavirus, Lassa Fever virus and Marburg virus families. The designed sequences include those which comprise the designed Tri-LEMvac vaccine, and the bivalent Lassa virus vaccine. Nucleic acid molecules encoding the polypeptides, as well as vectors, fusion proteins, pseudotyped virus particles, pharmaceutical compositions, combined preparations, cells, and their use as vaccines against viruses of the Ebolavirus, Lassa Fever virus and Marburg virus families are also described.

The embodiments disclosed herein utilize RNA targeting effectors to provide a robust CRISPR-based diagnostic for hemorrhagic fever virus applications. Embodiments disclosed herein can differentiate between hemorrhagic fever viruses that present with similar symptoms, as well as between strains of a hemorrhagic fever virus.

The invention relates to the field of virus detection, in particular to a kit for rapidly detecting multiple pathogens of hemorrhagic fever, which comprises a dengue virus upstream primer, a dengue virus downstream primer and a probe for detecting the dengue virus. The kit comprises a chikungunya virus upstream primer, a chikungunya virus downstream primer and a probe for detecting the chikungunya virus, a Zika virus upstream primer, a Zika virus downstream primer and a probe for detecting the Zika virus; the kit comprises a yellow fever virus upstream primer, a yellow fever virus downstream primer and a probe for detecting the yellow fever virus. The kit provided by the invention can quickly and accurately detect and distinguish the hemorrhagic fever pathogen, and the actual detection time is only 24-35 minutes, which is far lower than that of the kit in the prior art.

The present invention provides a novel method of treating viral hemorrhagic fever, including hemorrhagic fever with renal syndrome, which is treated or ameliorated by administering a neutrophil elastase inhibitor. When the neutrophil elastase inhibitor is used for treating hemorrhagic fever with renal syndrome, inflammatory response can be inhibited, endothelium and organ injury can be reduced, death rate can be reduced, and complications can be reduced.

The application discloses a kind of target sequencing primer set and kit for detecting multiple rodent carrying pathogenic microorganisms.The nucleotide sequence of the primer set is shown as SEQ ID NO:1-400.The kit comprises the primer set shown as SEQ ID NO:1-400.The application can simultaneously detect 50 kinds of rodent carrying pathogenic microorganisms, with the advantages of high detection throughput, clear pathogen spectrum range, low sequencing cost, high sensitivity, and no influence of host nucleic acid, etc.The target sequencing primer set and kit are developed for common pathogen spectrum carried by rodents, which can effectively make up for the shortcomings of prior art, expand the detection range, improve the detection sensitivity, and provide key technical support for early warning, prevention and control of plague, hemorrhagic fever with renal syndrome, fever with thrombocytopenia syndrome and other rodent-borne diseases.

The invention discloses a Marburg hemorrhagic fever suckling mouse infection model capable of being operated in a biosafety secondary laboratory and application of the Marburg hemorrhagic fever suckling mouse infection model, and belongs to the technical field of biology. According to the invention, based on a VSV reverse genetics technology platform, a recombinant VSV-Angola expressing Marburg virus glycoprotein (GP) is constructed, the VSV-Angola is used as an alternative virus to cause lethal infection of 3-day-old BALB / c suckling rats, and a Marburg virus (MARV) alternative animal infection model adapted to biosafety secondary (BSL-2) laboratory operation is successfully developed. According to the infection model, the space load and the operation risk of a biosafety secondary laboratory are greatly reduced, a standardized neck subcutaneous inoculation technology is supported, the repeatability and the safety of an experiment are remarkably improved, the expenditure budget of an experimental animal is saved, and the infection model is suitable for research on a neuropathogenic mechanism and blood brain barrier penetrability of an antiviral drug; and the method has high cost benefit and clinical transformation value.