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HTNV-NP (Hantaan virus nucleoprotein)-specific CTL (cytotoxic T lymphocyte) epitope peptides and application thereof

An epitope peptide and specific technology, which is applied in the field of CTL epitope peptides restricted by HLA-I molecules, can solve the problems of non-specific prevention and treatment methods, and achieve good development and application prospects

Active Publication Date: 2012-04-04
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0008] HFRS caused by HTNV infection has high morbidity and mortality, but so far there is no specific prevention and treatment

Method used

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  • HTNV-NP (Hantaan virus nucleoprotein)-specific CTL (cytotoxic T lymphocyte) epitope peptides and application thereof
  • HTNV-NP (Hantaan virus nucleoprotein)-specific CTL (cytotoxic T lymphocyte) epitope peptides and application thereof
  • HTNV-NP (Hantaan virus nucleoprotein)-specific CTL (cytotoxic T lymphocyte) epitope peptides and application thereof

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Embodiment Construction

[0032] The present invention first synthesizes partially overlapping 15 peptides covering the full length of the NP region of the HTNV 76-118 strain, and uses ELISPOT and magnetic bead separation techniques to systematically identify the CD4 on the NP + and CD8 + T cell 15 peptide epitopes, combined with bioinformatics SYFPEITHI, ANN, BIMAS and other T cell epitope prediction software predictions, select and synthesize multiple 9 peptides that may induce the body to produce CTL effects. ELISPOT technology was used to evaluate its immunogenicity, and it was identified that it could induce CD8 + CTL 9 peptide epitopes for T cell responses. Epstein-Barr virus (EBV) was used to transform PBMCs of HFRS patients, and EBV-transformed B-LCLs were established, and B-LCLs loaded with all or part of the HLA class I alleles of the corresponding nine peptides were used as antigen-presenting cells. Magnetic bead separation of CD8 + T cells were used as effector cells, and ELISPOT was use...

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Abstract

The invention discloses HTNV-NP (Hantaan virus nucleoprotein)-specific CTL (cytotoxic T lymphocyte) epitope peptides and application thereof. The CTL epitope peptides have amino acid sequences shown in SEQ ID NO:1-12. Especially, HLA-I (human leucocyte antigen-I) molecule restricted epitope polypeptide in the HTNV-NP-specific CTL epitope peptides can induce CD8+T lymphocyte to generate strong cellular immune response and secrete high-level IFN-gamma. The HTNV-NP-specific CTL epitope peptides can be used for preparing CTL epitope peptide vaccines or for inducing the generation of CTL epitope peptide-specific CTL, or for preparing CTL epitope peptide-sensitized antigen presenting cells and have bright development and application prospects in the field of specific immunization therapy of HFRS (hemorrhagic fever with renal syndrome).

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of Hantaan virus, and relates to HTNV-NP-specific CTL epitope peptides and applications thereof, in particular to CTL epitope peptides restricted by HLA-I class molecules. Background technique [0002] Hantaan virus (HTNV) is an important virus among the pathogenic microorganisms within the scope of verification of the Protocol on the Prohibition of Biological Weapons. It belongs to the genus Hantavirus (HTV) of the Bunyaviridae family and is the prototype of HTV. HTV viruses are all single-stranded negative-sense RNA enveloped viruses. The genome includes three segments: large (L), medium (M), and small (S). Among them, the L segment encodes RNA polymerase, and the M segment encodes envelope glycoprotein. 1 and 2 (glycoprotein 1, G1 / Gn and glycoprotein 2, G2 / Gc), the S fragment encodes nucleocapsid protein (NP). The HTNV 76-118 strain first isolated by Li Haowang et al. in 1978 ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N5/0783C12N5/0781A61K39/12A61P31/14
Inventor 金伯泉马樱王美亮庄然徐竹蔚张赟张春梅张宇丝刘蓓易静
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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