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Use of anti-hantavirus medicament arbidol

A technology of Hantavirus and Arbidol, which is applied in the application field of anti-Hantavirus drug Arbidol, achieves the effects of good tolerance and convenient use

Inactive Publication Date: 2009-06-24
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We used new synthetic methods and raw materials to synthesize a new drug (CN200510018613.9) with Chinese intellectual property rights, and proved that the drug has anti-Coxsackie virus B3, Coxsackie virus B5, adenovirus, and respiratory syncytia in animals. The role of respiratory viruses such as viruses and rhinoviruses, but its effect on hantavirus has not been reported and discovered

Method used

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  • Use of anti-hantavirus medicament arbidol
  • Use of anti-hantavirus medicament arbidol
  • Use of anti-hantavirus medicament arbidol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Arbidol is to the toxic effect of Vero E6 cell

[0029] According to the previous synthesis process (an anti-respiratory virus drug and its application, patent number: CN200510018613.9), we synthesized Arbidol, and tested by high performance liquid phase that there are no impurities and other components. Arbidol was dissolved in dimethyl sulfoxide (DMSO), then dissolved in double distilled water to make a 200 μg / ml stock solution, sterilized by filtration, and stored at 4°C. Dilute Arbidol to different concentrations with MEM cell maintenance solution containing 2% calf serum, add 200 μl to the monolayer Vero E6 cells in a 96-well culture plate, and set a cell control at the same time, observe the morphological changes of the cells after 10 days Live cell staining was carried out by the MTT method, and the OD570 value was read, and the cell survival rate was calculated according to the OD value to judge the cytotoxicity of different concentrations of drugs...

Embodiment 2

[0031] Embodiment 2: Arbidol anti-Hantan virus effect in vitro

[0032] Seed Vero-E6 cells into a 6-well plate and wait to grow into a monolayer. Arbidol, which is double-diluted at 0.5-8 μg / ml, is administered in different ways. The details are as follows, I (the protective effect of the drug on the cells): after the drug acts on the cells at 37°C for 2 hours, discard the drug-containing maintenance solution, wash twice with PBS, absorb the virus at 37°C for 2 hours, and replace the cell maintenance solution after washing twice; II (direct killing): the virus solution reacts with different concentrations of drugs at 37°C for 2 hours, then incubates the mixture with the cells at 37°C for 2 hours, discards the mixture, washes twice with PBS, and replaces the cell maintenance solution; III (inhibition of virus replication): virus solution Adsorbed cells were incubated at 37°C for 2 hours, washed twice, and drug-containing maintenance solution was added; at the same time, uninfe...

Embodiment 3

[0041] Embodiment 3: Arbidol is to the toxic effect of suckling mouse

[0042] Do the maximum tolerated dose test first. Maximum tolerated dose (MTD) test: namely administer once at the maximum allowable concentration and the maximum volume that the animal can tolerate, observe for 14 days, and record the reaction of the animal. If the animal does not appear death or poisoning symptoms during the observation period, it is considered that the MTD of the drug on a certain route of administration to a certain animal is a certain value (mg kg -1 or g·kg -1 ).

[0043] Oral administration of Arbidol, 1mg·kg -1 , 0.5ml·10g -1 The rats were observed for 14 days to observe whether the animals were poisoned or died, and it was decided whether to carry out acute toxicity and subacute tests according to the results of the maximum tolerated dose test.

[0044] Balb / C1-day-old suckling mice orally administered 1mg·kg -1 , There was no poisoning and death in animals within 14 days, in...

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Abstract

The invention discloses application of a medicine in resisting Hantaan viruses, namely arbidol. The arbidol has obvious effect of inhibiting the Hantaan viruses in vitro, and the antivirus effect of drug administration before the viruses enter a cell is stronger than that of the drug administration after the viruses enter the cell. The concrete embodiment comprises the following: the drug administration is performed before and after the infection, the positive rate of virus-infected cells and the fluorescence intensity are reduced along with the concentration increase, and the medicine has dosage effect; and the medicine can obviously reduce the positive rate of virus infection, and the mRNA expression of the viruses is reduced. The arbidol has protection and treatment effect on the infection of the Hantaan viruses on a suckling mouse, and the protection effect is stronger than the treatment effect. The drug administration is performed within 24h before the infection; and with the increase of the dosage of the medicine, the death rate of the mouse is reduced, and the average survival days are extended. The drug administration within 24h after the infection can not improve the survival rate of an animal, but can extend the average survival days of the animal. The arbidol has the effect of inhibiting the Hantaan viruses in a body of the animal. The drug administration within 24h before the infection can lighten the pathologic change of tissues (lung, kidney, and brain), and has treatment effect on HFRS. The medicine has prevention effect and also has treatment effect on patients with the hemorrhagic fever with renal syndrome (HFRS) caused by the Hantaan viruses, and no toxic side effect is found.

Description

technical field [0001] The present invention relates to a kind of antiviral medicine, more specifically relate to a kind of Arbidol anti-hantavirus medicine, specifically, the present invention is a kind of new compound with good antiviral activity, and it can be in cell and animal body And the anti-hantavirus effect in the human body, the drug has both preventive and therapeutic effects on hemorrhagic fever with renal syndrome (HFRS) caused by hantavirus, and no toxic and side effects have been found. Background technique [0002] Hemorrhagic fever with renal syndrome (HFRS) is a natural foci disease caused by Hantaviruses (HV) and transmitted by rodents. Infection, the disease has a high mortality rate and is very harmful. It was once listed by the World Health Organization (WHO) as a potential biological weapon that can be transmitted through aerosols. The number of cases in China accounts for 90.94% of the total number of cases in the world, and it is a severe epidemic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61P31/12A61P13/12
Inventor 杨占秋邓海英罗凡石丽桥钟琼刘颖娟王又又肖红
Owner WUHAN UNIV
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