Method for detecting whole-blood sample coagulation item using magnetic-bead method

A whole blood sample, magnetic bead method, applied in the field of medical testing, can solve the problem of inapplicability of effective rescue and other problems, achieve the effect of easy to master, save equipment, and reduce the chance of errors

Inactive Publication Date: 2007-03-28
MEIDE TAIPINGYANG PRECISION INSTR MFG
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional coagulation test is determined by plasma samples, which can accurately and sensitively reflect the secondary hemostatic response of clinical patients, but requires certain conditions (such as centrifugation, etc.), an

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] When the activated partial thromboplastin time (APTT) needs to be determined, a fresh sodium citrate anticoagulated whole blood sample is placed on the coagulometer, and the activated partial thromboplastin time reagent (APTT-WB ) for determination, the amount of sample used and the amount of reagent added are the same as those of the traditional plasma method; the activated partial thromboplastin reagent (APTT-WB) is 1 ml of APTT reagent dissolved in 0.93 ml of APTT reconstituted solution.

[0013] When the prothrombin time (PT) needs to be determined, the fresh sodium citrate anticoagulated whole blood sample is placed on the coagulation instrument, and the prothrombin time reagent (PT-WB) is used for the determination of the whole blood by the magnetic bead method and the whole blood. For determination, the amount of sample used and the amount of reagent added are the same as those of the traditional plasma method; the prothrombin time reagent (PT-WB) used for whole b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

One kind blood samples detection method using magnetic beads, completed with the following steps: (1) Get fresh clinical samples; (2)Put these specimen on coagulation analyzer, use magnetic beads and special adjustment reagents aiming at the whole blood specimens, measure four coagulations: Activated partial thromboplastin time (APTT), Prothrombin time (PT), Prothrombin time (TT), Fibrinogen (FIB). The advantages of this invention are: 1.The method is simple, time-saving and easy to technical staff. 2. Reduce costs and save centrifuges and other equipment, reduce errors; 3. It is efficient and suitable for the battlefield, natural disasters and remote mountainous areas, medical teams. Particularly, it's suitable for hemorrhagic diseases and natural disasters (such as hemophilia, etc.). 4. Save specimen volume. 5. Reflect the level of specimens of blood coagulation preciously and accurately.

Description

[0001] Field: [0002] The invention belongs to a medical testing method, in particular to a method for detecting coagulation items of whole blood samples by using a magnetic bead method. Background technique: [0003] The traditional coagulation test is determined by plasma samples, which can accurately and sensitively reflect the secondary hemostatic response of clinical patients, but requires certain conditions (such as centrifugation and other processing), and is not suitable for laboratories with poor conditions, combat readiness, and natural disasters, especially It is an effective aid for patients with bleeding diseases (such as hemophilia, etc.) in battlefield first aid and natural disaster first aid. Invention content: [0004] The purpose of the present invention is to overcome the deficiencies existing in the above-mentioned prior art, and to provide a method for detecting coagulation items of whole blood samples by using the magnetic bead method. Specimen volume...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/49
Inventor 任峰张桂珍
Owner MEIDE TAIPINGYANG PRECISION INSTR MFG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap