Hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit and preparation method and detection method thereof

Abstract

The invention provides a hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit. The hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit comprises an upper shell, a lower shell, an immunostrip, test paper and the like; a gold-labeled composite containing a hemoglobin monoclonal antibody, a hemoglobin-haptoglobin composite monoclonal antibody and a transferrin monoclonal antibody is scribed on a nitrocellulose membrane; 3 test lines, a quality control line (15) and a gold-labeled composite membrane scribing line (8) are arranged on the nitrocellulose membrane in parallel. The invention further provides a preparation method and a detection method of the hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit. The hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit is convenient and simple in operation, stable in performance and accurate in result, has a relatively good reference value for early diagnosis and identification of colorectal cancer or colon cancer tumor or other tumors with lower gastrointestinal bleeding symptoms in clinic, significantly improves the positive detection rate of digestive hemorrhagic diseases, is suitable for clinical hospital examination and household self-examination, and provides measures for large-scale general examination of such diseases.

Description

technical field [0001] The invention belongs to the technical field of clinical in vitro diagnostic reagents, specifically a hemoglobin, hemoglobin-haptoglobin complex, and transferrin joint detection kit, which applies the principle of immunology, lateral flow chromatography and chemical o-toluidine chromogenic method, By detecting hemoglobin, hemoglobin-haptoglobin complex and transferrin in human feces, the symptoms of lower gastrointestinal bleeding can be found, and early diagnosis of lower gastrointestinal tumors such as rectal cancer or colon cancer can be performed. Background technique [0002] Hemoglobin (Hb, Hemoglobin) in feces, also known as fecal occult blood (FOB, Fecal Occult Blood), is currently clinically regarded as one of the tumor markers for the diagnosis of rectal cancer and colon cancer. Haptoglobin (Hp, Haptoglobin, also known as haptoglobin or globulin) is widely present in the blood and other body fluids of humans and many mammals, and the content ...

AI Technical Summary

Problems solved by technology

For example, the Chinese invention patent application publication number is CN101965515A (announced on February 2, 2011) providing a method and device for detecting occult blood, including detecting transferrin and hemoglobin in GI samples, and detecting The amounts of transferrin and hemoglobin are compared with the respective predetermined values ​​of transferrin and hemoglobin, thereby topping the bleeding from the GI and providing detection of transferrin and hemoglobin using a lateral flow sandwich immunoassay device , the technical scheme lacks the detection of hemoglobin-haptoglobin complex, and the positive coincidence rate for rectal or colon cancer is low

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