Oral vaccine for hemorrhagic disease of grass carp as well as preparation and application of oral vaccine

A grass carp and reovirus technology, applied in vaccines, blood diseases, veterinary vaccines, etc., can solve problems such as low conversion rate and genetic instability limitation

Inactive Publication Date: 2018-12-21
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Although Bacillus subtilis has good research value for microbial vaccines, the low conversion rate and geneti

Method used

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  • Oral vaccine for hemorrhagic disease of grass carp as well as preparation and application of oral vaccine
  • Oral vaccine for hemorrhagic disease of grass carp as well as preparation and application of oral vaccine
  • Oral vaccine for hemorrhagic disease of grass carp as well as preparation and application of oral vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation of embodiment 1 grass carp haemorrhagic disease oral vaccine

[0083] 1. Construction of Bacillus subtilis / Escherichia coli shuttle expression system

[0084] The recombinant plasmid pVAX1-S6 containing the S6 target fragment was constructed and preserved by the Pearl River Fisheries Research Institute of the Chinese Academy of Fishery Sciences in the early stage. The build is saved and served. The recombinant shuttle plasmid pEB03-CotC-S6 was obtained by homologous recombination. First, the PMY fragment was excised from the recombinant plasmid pEB03-CotC-PMY (containing Hind III and Sac I restriction sites) with Hind III and Sac I enzymes , and then the S6 target fragment was connected to the linearized vector pEB03-CotC to obtain the recombinant shuttle plasmid pEB03-CotC-S6. At the same time, the recombinant plasmid pEB03-CotC was used as a negative control. The shuttle expression plasmids pEB03-CotC-S6 and pEB03-CotC were transformed into Bacillus...

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Abstract

The invention belongs to the technical field of biology and particularly relates to an oral vaccine for hemorrhagic disease of grass carp as well as preparation and application of the oral vaccine. The invention firstly provides fusion protein containing bacillus subtilis spore capsid protein CotC and grass carp reovirus VP4 protein, wherein the amino acid sequence of the grass carp reovirus VP4 protein corresponds to the amino acid sequence encoded by the nucleotide sequence shown as SEQ ID NO.8. The oral vaccine prepared by the fusion protein has the advantages of simple preparation process,low cost, convenience in oral administration and the like; meanwhile, the damage of fishing and injecting to the fish bodies in the injection immunization scheme is avoided. In addition, the oral vaccine has better immune protection effect on fish body resistance to of the hemorrhagic disease of the grass carp compared with an existing vaccine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an oral grass carp hemorrhagic disease vaccine and its preparation and application. Background technique [0002] Grass carp hemorrhage (Grass Carp Hemorrhage) is a viral infectious disease caused by Grass carp reovirus (GCRV) that endangers grass carp farming. Bleeding symptoms, hence the name. It was first discovered in 1970, and has since been popular in various breeding areas in Hubei, Hunan, Guangdong, Guangxi, Jiangsu, Zhejiang, Anhui, Fujian, Shanghai, Sichuan and other provinces, cities, and autonomous regions in China. It can also infect herring, crucian carp, wheat ear carp, etc. Various fish species. Because grass carp haemorrhagic disease harms many species of fish, the epidemic range is wide, the onset season is long (the main epidemic season is from late June to the end of September every year), and the mortality rate is as high as 85%, which brings great ...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62C12N15/75A61K39/15A61P31/14A61P7/04
CPCA61K39/12A61P7/04A61P31/14C12N15/62C12N15/75C07K14/005C07K14/32A61K2039/552A61K2039/523C07K2319/00C12N2720/12034C12N2720/12022
Inventor 余新炳姜红烨林志鹏周心怡边青
Owner SUN YAT SEN UNIV
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