Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof

Abstract

The invention relates to a colloid gold duplex test strip for detecting fecal occult blood and a preparation method thereof. The colloid gold duplex test strip is composed of a bottom lining, a sample pad, a marker pad, a coating film and bibulous paper, wherein the sample pad, the marker pad, the coating film and the bibulous paper are sequentially adhered to the bottom lining in a lap joint mode. The marker pad comprises a colloid gold marked mouse against human Hb monoclonal antibody Hb1 and a mouse against human Tf monoclonal antibody Tf1, the concentration of the mouse against human Hb monoclonal antibody Hb1 is 5-20 Mu g / mL, and the concentration of the mouse against human Tf monoclonal antibody Tf1 is 5-20 Mu g / mL. According to the colloid gold duplex test strip, hemoglobin and transferrin in faeces are detected synchronously, the specificity is high, the sensitivity is high (the detection limit of Hb is 200 ng / ml, and the detection limit of Tf is 40 ng / ml respectively), the positive detection rate of the digestive tract hemorrhagic disease is improved remarkably, the asymptomatic, slight and continuous alimentary tract hemorrhage which cannot be seen by naked eyes and a microscope can be accurately detected, and the colloid gold duplex test strip can avoid the interference of medicine, such as animal blood and so on.

Description

(1) Technical field [0001] The invention relates to a colloidal gold double-linked test strip for detecting fecal occult blood and a preparation method thereof. (2) Background technology [0002] Fecal occult blood refers to a small amount of bleeding in the digestive tract, the red blood cells are digested and destroyed, the appearance of the stool does not change abnormally, and the bleeding cannot be confirmed by naked eyes and microscopes. Fecal occult blood detection is one of the routine stool test items, which has important reference value for the diagnosis and differential diagnosis of gastrointestinal bleeding and gastrointestinal tumors, and is often used as a screening index for diagnosing gastrointestinal bleeding. [0003] Fecal occult blood detection usually has two kinds of chemical method and immunological method. Among the chemical detection methods based on guaiac ester, benzidine, o-toluidine, reduced phenolphthalein, tetramethylbenzidine and other chromo...

AI Technical Summary

Problems solved by technology

[0005] Wanhua Puman Company took the lead in developing a new method for detecting fecal occult blood with TF / Hb double-linked colloidal gold diagnostic test strips, integrating anti-human TF antibody and anti-human Hb antibody on the same test strip (two test strips spliced ​​together, There are two injection ports), which can detect TF and Hb simultaneously without interfering with each other. The advantages of the two are complementary, but the detection limit of Hb is limited (tested, 20μg / ml can be detected, 1μg / ml cannot be detected), Sensitivity and detection limit need to be further improved

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