Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof

A colloidal gold, double test technology, applied in measurement devices, instruments, scientific instruments and other directions, can solve the problems of non-detection, the sensitivity and detection limit need to be further improved, and the Hb detection limit is limited, and achieves positive detection. The effect of improving the rate, accurate detection of asymptomatic, and high sensitivity

Inactive Publication Date: 2016-04-06
XIAMEN INST OF RARE EARTH MATERIALS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Wanhua Puman Company took the lead in developing a new method for detecting fecal occult blood with TF/Hb double-linked colloidal gold diagnostic test strips, integrating anti-human TF antibody and anti-human Hb antibody on the same test strip (two test strips spliced ​​together, There are tw...

Method used

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  • Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof
  • Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof
  • Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof

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Embodiment 1

[0026] The principle of double-antibody sandwich immunochromatography is used to detect trace amounts of hemoglobin and transferrin in human feces.

[0027] like figure 1 As shown, in this embodiment, the sample pad 1, the marking pad 2, the coating film 3 and the absorbent paper 4 are successively overlapped on the substrate 7, and the sample pad 1 is a sample application area, which is used to absorb the sample to be tested for fecal occult blood. , the coating film 3 is provided with detection lines and quality control lines.

[0028] Colloidal gold-labeled mouse anti-human Hb monoclonal antibody Hb1 (15 μg / mL) and mouse anti-human Tf monoclonal antibody Tf1 (18 μg / mL) are used on the marker pad 2; detection line 1 and detection line 2 of coating film 3 Coat another mouse anti-human Hb monoclonal antibody Hb2 (1.8mg / mL) and mouse anti-human Tf monoclonal antibody Tf2 (1.5mg / mL) respectively. The quality control line of the coated membrane 3 was coated with goat anti-mouse...

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Abstract

The invention relates to a colloid gold duplex test strip for detecting fecal occult blood and a preparation method thereof. The colloid gold duplex test strip is composed of a bottom lining, a sample pad, a marker pad, a coating film and bibulous paper, wherein the sample pad, the marker pad, the coating film and the bibulous paper are sequentially adhered to the bottom lining in a lap joint mode. The marker pad comprises a colloid gold marked mouse against human Hb monoclonal antibody Hb1 and a mouse against human Tf monoclonal antibody Tf1, the concentration of the mouse against human Hb monoclonal antibody Hb1 is 5-20 Mu g/mL, and the concentration of the mouse against human Tf monoclonal antibody Tf1 is 5-20 Mu g/mL. According to the colloid gold duplex test strip, hemoglobin and transferrin in faeces are detected synchronously, the specificity is high, the sensitivity is high (the detection limit of Hb is 200 ng/ml, and the detection limit of Tf is 40 ng/ml respectively), the positive detection rate of the digestive tract hemorrhagic disease is improved remarkably, the asymptomatic, slight and continuous alimentary tract hemorrhage which cannot be seen by naked eyes and a microscope can be accurately detected, and the colloid gold duplex test strip can avoid the interference of medicine, such as animal blood and so on.

Description

(1) Technical field [0001] The invention relates to a colloidal gold double-linked test strip for detecting fecal occult blood and a preparation method thereof. (2) Background technology [0002] Fecal occult blood refers to a small amount of bleeding in the digestive tract, the red blood cells are digested and destroyed, the appearance of the stool does not change abnormally, and the bleeding cannot be confirmed by naked eyes and microscopes. Fecal occult blood detection is one of the routine stool test items, which has important reference value for the diagnosis and differential diagnosis of gastrointestinal bleeding and gastrointestinal tumors, and is often used as a screening index for diagnosing gastrointestinal bleeding. [0003] Fecal occult blood detection usually has two kinds of chemical method and immunological method. Among the chemical detection methods based on guaiac ester, benzidine, o-toluidine, reduced phenolphthalein, tetramethylbenzidine and other chromo...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/577G01N33/558
Inventor 张云张肖胡亮王选东杨福太李文斌
Owner XIAMEN INST OF RARE EARTH MATERIALS
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