Recombinant human-like collagen and production method thereof

Abstract

The invention discloses a recombinant human-like collagen. The recombinant human-like collagen has high hydrophilicity and stability. The structure of the recombinant human-like collagen is totally the same with the structure of the corresponding part of a natural collagen gene sequence. When the recombinant human-like collagen is applied to a human body, immunological rejection cannot be caused. The recombinant human-like collagen can be widely applied to the fields of biomedical materials, cosmetics and the like. The invention also discloses a production method of the recombinant human-like collagen, which comprises the following steps of: constructing pichia pastoris gene engineering bacteria; fermenting and cultivating the pichia pastoris gene engineering bacteria; inducing and expressing the recombinant human-like collagen; and purifying the recombinant human-like collagen. The human-like collagen produced by the method has high expression and is convenient to purify. The expression in a fermentation tank can achieve 5.0g/L. In a single batch, about 150g of crude collagens can be produced by a tank with the volume of 50L. Due to high expression, the purity of the crude collagens is over 80 percent and few impurity proteins exist.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a recombinant humanoid collagen and a production method thereof. Background technique [0002] Collagen is a group of protein families widely distributed in the connective tissue of the human body. It is also the most abundant protein in the body, accounting for about 25% to 33% of the total protein. It plays an important role in maintaining the normal physiology of cells, tissues and organs. Function and damage repair play an important role. Collagen, as a natural biological resource, has biocompatibility and biodegradability unmatched by synthetic polymer materials, and can be widely used in industries such as medicine and cosmetics. The extracted collagen is a mixture of various collagen peptides with molecular weights ranging from large to small. The properties are very complex and difficult to further process, which limits its application as a biomat...

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Problems solved by technology

However, the bacterial expression system is likely to cause pyrogen residues, resulting in impure expression products, which are difficult to apply clinically. Moreover, the target protein is usually expressed in the form of inclusion bodies, and the product

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