145 results about "Neutral red stain" patented technology

The invention relates to preparation of a magnetic nano adsorbent and a method for removing organic cationic dyes from waste water by the same, and belongs to the technical field of wastewater treatment. The magnetic nano adsorbent uses Fe3O4 particles as a carrier, and coated humic acid as an active adsorbing component. The method comprises the following steps: adding mixed solution of ferric chloride and ferrous sulfate to alkaline humic acid solution, reacting under anaerobic, heating and stirring conditions, and obtaining the magnetic nano adsorbent after magnetic separation, washing, drying and grinding. The removal rate of methylene blue and neutral red reaches more than 95% by treating organic cationic dye wastewater with the magnetic nano adsorbent. The magnetic nano adsorbent after magnetic solid-liquid separation is regenerated by methanol-acetic acid solution, a desorption solvent recovers methanol and acetic acid by distillation for recycling use. The magnetic nano adsorbent has the advantages of nanoscale size, high active surface radicals, extremely high adsorption removal rate for cationic dyes, simple process, high efficiency and easy control.

The invention discloses an immobilized biological adsorbent containing phanerochete chrysosporium, the surface of which is uniformly wrapped by a layer of carrier Fe3O4 nanoparticles for fixation. The method comprises the steps of: first adding Fe3O4 nanoparticles into a phanerochete chrysosporium liquid medium containing tween 80, then inoculating a phanerochete chrysosporium spore suspension, conducting shaking cultivation so as to obtain the immobilized biological adsorbent. The invention also discloses application of the adsorbent in dye wastewater treatment. The biological adsorbent of the invention has high adsorption efficiency and large adsorption capacity, and can be effectively used for treating neutral red and lead in dye wastewater. The preparation technology and treatment technology in the invention are characterized by simplicity and low cost.

The invention provides a kit and method for inducing differentiation from bone mesenchymal stem cells to osteoblasts. The kit comprises an osteoblast inducing culture solution and an osteoblast identification solution, wherein the inducing culture solution comprises a cell culture solution A, a cell culture solution B, a cell culture solution C, a cell culture solution D and a cell culture solution E; the osteoblast identification solution comprises a cell immobilizing solution, coloring agents and a detergent; the cell culture solution A is an alpha-minimum essential medium (MEM) liquid culture medium; the cell culture solution B is fetal bovine serum; the cell culture solution C is a dexamethasone solution; the cell culture solution D is a beta-sodium glycerophosphate solution; the cell culture solution E is a vitamin C solution; the cell immobilizing solution is 4% paraformaldehyde; the coloring agents include sodium thiosulfate, silver nitrate and neutral red; and the detergent is double distilled water.

The invention relates to a method for mensurating cytotoxicity of mainstream cigarette smoke. The method is characterized in comprising the following steps: firstly, a cultured cell is directly put to the mainstream cigarette smoke diluted by clean air and is exposed; secondly, the cell is subjected to neutral red staining; according to the situation that the cell is ingested with neutral red, a spectrophotometer of a readable porous culturing plate is utilized to mensurate an absorbance value; and the cell is compared with a contrastive sample exposed in the clean air, thereby judging the toxicity of the mainstream cigarette smoke. The method is based on a testing principle that the cultured cell is directly put to the mainstream cigarette smoke and is exposed; the level for ingesting the neutral red by the cell is in positive proportion to the number of viable cells; when the toxic effect of the mainstream cigarette smoke is larger, the survival cells exposed in the mainstream cigarette smoke is fewer, correspondingly the dye absorption amount is less and the absorbance value is lower. The method can quantitatively evaluate the cytotoxicity of a tested object, has the characteristics of simple operation, high sensitivity and reliable result, and creates a new method for mensurating the cytotoxicity of the mainstream cigarette smoke.

The invention relates to a novel method for measuring iron without mercury, which comprises the following steps of: decomposing a test sample through mixed acid of hydrochloric acid, sulfuric acid and phosphoric acid; taking neutral red and sodium diphenylaminesulfonate as indicators; measuring the iron through a SnCl2-TiCl3-K2Cr2O7 volumetric method; reducing Fe<3+> of phi Fe<3+>/ Fe<2+>=+0.76V through tin bichloride with phi Sn<4+>/ Sn<2+>=+0.16V; adding neutral red reagent when the solution is flavescent, i.e. the solution still contains a small amount of Fe<3+>; taking the color change of the neutral red as an indicator indicating that Fe<3+> is completely reduced to be Fe<2+> according to the principle that the neutral red is reduced to be colorless after the Fe<3+> is reduced completely, and the neutral red reagent is blue in the Fe<3+> solution; dropping titanium trichloride with phi Ti<4+>/Ti <3+>=+0.1V until blue is disappeared; removing excess titanium trichloride through oxidation by a 5.000g. L<-1> sodium diphenylaminesulfonate-potassium dichromate standard solution method; and finally, calculating ion grade of the test sample.

The invention relates to the technical field of fish detection, and particularly relates to fish freshness detection test paper. Each liter of an indicator solution in a reagent layer contains 0.5-1g of bromophenol red, 0.6-1.1g of bromcresol purple, 0.3-0.5g of bromothymol blue, 0.3-0.8g of neutral red, 0.2-0.6g of phenol red and the balance of a solvent. The fish freshness is detected rapidly with low cost and high flexibility. The detection test paper can be used for detecting the freshness of chilled, refrigerated and frozen fish. The using method is simple; and only less amount of fish body surface mucus is dipped when the test paper is used, and the fish freshness can be obtained by comparing the color instantly developed by the test paper with a standard color chart.

The invention relates to the field of protein, in particular to a bioactive peptide SKVLPVPEKAVPYPQ as well as a preparation method and an application thereof. An amino acid sequence of the bioactive peptide SKVLPVPEKAVPYPQ is Ser-Lys-Val-Leu-Pro-Val-Pro-Glu-Lys-Ala-Val-Pro-Tyr-Pro-Gln. An in-vitro anti-oxidation experiment and an in-vitro immunity function promoting experiment prove that the bioactive peptide SKVLPVPEKAVPYPQ has better anti-oxidation biological activity and immunity regulation function, on one hand, the bioactive peptide SKVLPVPEKAVPYPQ has better anti-oxidation activity, can clear free radicals in an organism and improves life quality; on the other hand, by the aid of the bioactive peptide SKVLPVPEKAVPYPQ, in-vitro proliferation capacity of lymphocytes and macrophages can be enhanced, the phagocytosis capacity of the macrophages for neutral red is improved, the organism's capacity of resisting outside pathogen infection is improved, morbidity of the organism is reduced, and therefore, the bioactive peptide SKVLPVPEKAVPYPQ has quite great significance in development of food, healthcare products and drugs with an immune regulation function and an anti-oxidation function.

The invention belongs to the technical field of food, and more specifically relates to a color/fluorescence double signal visible rapid nitrite detection method, and applications thereof. The color/fluorescence double signal visible rapid nitrite detection method comprises following steps: firstly, a carbon dot-neutral red composite system is prepared, wherein carbon dot synthesis and constructionof the carbon dot-neutral red composite system are carried out; a common filter paper is immersed in the carbon dot-neutral red composite system so as to obtain a carbon dot-neutral red composite system detection test paper; a sodium nitrite standard solution is prepared; a color standard colourimetric card and a fluorescence standard colourimetric card of nitrites are prepared; a software is adopted to extract the RGB values of each test paper, a matrix is constructed, and a multivariate linear regression model is constructed; and the content of nitrites in food to be detected is detected. The carbon dot-neutral red composite system possess color/fluorescence double response capacity on nitrites; detection operation steps are simple; the visible results are clear, visual, and accurate; the detection limit is lower; and it is promising for the color/fluorescence double signal visible rapid nitrite detection method to be used in large-scale food nitrite content detection.

A method to measure redox potentials and concentrations of redox active substances in an aqueous solution is described. The method is based on measurements of transmembrane electric potential through an electroconductive polymer membrane, for example, through a d,l-camphor sulfonic acid (CSA) doped polyaniline (PANI) membrane. Transmembrane electric potential demonstrates good Nernstian response as a function of redox potential in solutions,of the redox couples of Fe²⁺/Fe³⁺ and Fe(CN)₆⁴⁻/Fe(CN)₆³⁻. The membrane gives good response to redox active substances such as L-Ascorbic acid and redox active dyes Neutral red, Nile blue and N-phenylanthranilic acid that do not induce satisfactory response on Pt electrode. The lower detection limits can be as low as 0.2 mM. In the absence of redox processes it is also possible to measure Cl⁻ concentration at least from 0.05 mM to 100 mM.

The invention provides a humidity indicator which is really effective in environmental protection and comprises a carrier and solution used for soaking the carrier; wherein, the solution takes 30-80% of ethanol as a solvent and contains 0.00862-0.00992% of acid-base indicator and 0.8-13.8% of hygroscopic salt. The acid-base indicator is one of bromocresol green, bromothymol blue, thymol blue, thymolphthalein, bromophenol blue, methyl red, methyl orange, methyl yellow , neutral red and phenol red or the mixture thereof. The hygroscopic salt is alkali halide or alkali-earth metal halide. The humidity indicator of the invention has wide indicating range (capable of indicating 5-90% of ambient humidity) and high sensitivity. Diverse humidity indicators different in discoloration can be manufactured by adjusting the components. The humidity indicator of the invention has evident discoloration effect, simple use, reutilization, good suitability and extensive application.

The invention provides a three-dimensional graphene/Fe3O4 magnetic nanometer adsorption material. The material is obtained by ultrasonically crushing graphene oxide, adding Fe<3+> and L-Cys and performing one-pot coprecipitation under an alkaline condition. Characterization by a scanning electron microscope, a transmission electron microscope, X-ray diffraction, infrared spectroscopy, N2 adsorption and desorption and other methods prove that the three-dimensional graphene grafted with Fe3O4 magnetic nanoparticles has a good three-dimensional structure. The nanometer material synthesis method is simple and low in cost, has a large specific surface area, rich hydroxyl groups and free carboxyl groups and good magnetic properties, and can be used as an adsorption material for dye contaminantsin an aqueous solution. Adsorption property research is carried out by taking cationic methylene blue (MB), anionic Congo red (CR) and neutral type neutral red (NR) dyes as model molecules, and indicates that the material can effectively adsorb cationic, anionic and neutral dyes, and has potential applications in environmental pollutant adsorption materials.

Disclosed are methods using neutral red to mediate the interconversion of chemical and electrical energy. Electrically reduced neutral red has been found to promote cell growth and formation of reduced products by reversibly increasing the ratio of the reduced:oxidized forms of NAD(H) or NADP(H). Electrically reduced neutral red is able to serve as the sole source of reducing power for microbial cell growth. Neutral red is also able to promote conversion of chemical energy to electrical energy by facilitating the transfer of electrons from microbial reducing power to a fuel cell cathode.

The invention discloses a method for detecting melamine. The method comprises the following steps of: sequentially adding 0.05-5.0mL of 0.1-0.5g/L melamine solution, 1.0-2.5ml of 4.0*10<-6>mol/L eosin Y-solution, 0.5-1.0ml of 2.0*10<-4>mol/L neutral red solution and 0.3-1.0ml of Britton-Robinson buffer solution of which the pH value is 3.5 into a 10ml colorimetric tube, using the solution without melamine as the reagent blank, fixing the volume to a specific scale by using super-pure water, reacting for 30min in a water bath of 30 DEG C, subsequently respectively measuring the fluorescence value F538nm of the melamine-containing solution and the fluorescence value F0 of the reagent blank and calculating the difference value that deltaF538nm is equal to F538nm subtracted by the F0 value at 538nm on a fluorophotometer by using a 1cm quartz cuvette in a mode that 520nm is used as the excitation wavelength; preparing 30ml of milk, adding 20.0ml of 1% trichloroacetic acid solution and 8.0ml of 2% lead acetate solution, and carrying out ultrasound treatment for 20min; centrifuging a part of the solution for 10min, taking 1ml of the supernate, and measuring the fluorescence value and calculating the content of the melamine in the milk according to the same way. The method is simple, convenient and rapid.

The present invention provide a beta-1,3/1,6-glucan, a preparation method therefor, and application thereof in preparing immune enhancement and anti-tumor medicine and functional food. The preparation method comprises: drying and milling antarctic brown algae; after degreasing, water extracting, calcium chloride precipitating and anion exchanging resin purification, obtaining glucan with a beta-1,3-glucose main chain and a beta-1,6-glucose branched chain, wherein a molecular weight thereof is between 5-50 kDa. The beta-glucan prepared by the present invention has an immune enhancement activity, which is indicated by swallow of neutral red by in vitro macrophages and an NO release promoting experiment, and has a tumor growth inhibiting effect and a tumor metastasis inhibiting effect which are indicated by an in vivo animal experiment. According to the present invention, beta-1,3/1,6-glucan is prepared by using the antarctic brown algae; is characterized in richness and readily availability of raw material sources, simple preparation process, high product purity, strong bioactivity and easiness for industrialization; and has a prospect of being developed into immune enhancement and anti-tumor medicine.

The invention provides a method for measuring cytotoxicity of condensate of main stream smoke of cigarettes, which is characterized by comprising the following steps: 1) preparation of laboratory reagent, 2) preparation of the condensate of smoke, 3) cells inoculation and culture, 4) contamination of the condensate of smoke, 5) neutral red dying, and 6) result and analysis. The content of the method is as follows: on the basis of the principle of neutral red cell toxicity tests, test procedures are improved according to the cytotoxicity characteristics of the condensate of the main stream smoke of cigarettes, and a method for measuring cytotoxicity of condensate of main stream smoke of cigarettes with low hazard is established. In the invention, the step for fixing formaldehyde fixing solution is reduced to cause the test procedures to be simpler, and simultaneously, the condensate of the main stream smoke of cigarettes is a complex mixture system, has the toxic characteristics of low toxicity and the like and improves the diluted culture solution of samples, namely that the concentration of fetal calf serum is regulated from 10% to 5%, therefore, the test result is more accurate and reliable. The method has the advantages of simpler operation, higher sensitivity and more reliable results.

The invention discloses a method for fermented production of succinic acid on the basis of an electrochemical system for regulating intracellular reducing power regeneration. The method comprises three steps including strain activation, seed culture and anaerobic fermentation production of succinic acid, anaerobic fermentation adopts electrochemical fermentation, an electron carrier is added to a fermentation medium, the corresponding voltage is applied to a cathode, and the concentration of the electron carrier is in a range of 0.01-0.5 mmol/L. the electron carrier is neutral red or riboflavin. The cathode voltage applied to the cathode is scanned and determined in the range from 0.1 V to 1 V with the cyclic voltammetry at the scanning speed of 5-10 mV/s. When fermentation is performed in an electrochemical device under the anaerobic condition, the total amount of intracellular NADH is increased under the assistance of the electron carrier, the intracellular reducing power (NADH/NAD+) level is tripled, the succinic acid accumulation reaches 15.06 g/L, and efficient synthesis of the reduced product, namely, the succinic acid, is facilitated.

Reversibly organic thermochromic material comprising (by weight portion) neutral red 1-10 parts, aluminum oxide 10-100 parts, hexamethylene tetramine 10-100 parts, ferrous chloride 10-100 parts. The invention realized a low-temperature reversible organic heat off-color material prepared from neutral red as a novel color former, which can be used for preparing thermal color change printing ink and coating material.

The invention discloses a method for detecting cysteine by using a neutral red-gold composite material modified electrode. The method comprises: coating the surface of a glassy carbon electrode with the mixed solution of neutral red and chloroauric acid in a dropwise manner, naturally drying to form a film layer on the surface of the glassy carbon electrode so as to prepare the neutral red-gold composite material modified glassy carbon electrode, and detecting cysteine in a PBS buffer solution (pH value of 7) by using the neutral red-gold composite material modified glassy carbon electrode through a differential pulse voltammetry method, wherein the linear relationship between the electrode reduction peak current decreasing degree and the cysteine concentration is presented within a certain range, the linear range is 5*10<-6>-1*10<-4> mol/L, r is 0.998, and the detection limit is 2*10<-6> mol/L. According to the present invention, the preparation process of the modified electrode is simple, the cost is low, and the good application prospect is provided in the detection of cysteine.

The use of humic acids, zeolites, magnesium oxide beads and other mineral-containing materials in the activation of ethanol, alcoholic beverages and water is disclosed. Consumption of the energized liquids can have therapeutic benefits. The activated ethanol can be further used with neutral red dye and ultraviolet (UV) light illumination to indirectly enhance the environmental energy absorption properties of other liquids, including drinking water and of mineral containing materials. Mineral activated ethanol can similarly be used with neutral red dye and UV illumination to enhance the alternative cellular energy (ACE) pathway of an individual.

The invention provides a ratio type fluorescent carbon dot for Ag<+> and GSH detection and a preparation method thereof. The preparation method of the fluorescent carbon dot includes (1), weighing a certain mass of neutral red, dissolving the neutral red in secondary water, adding a small quantity of triethylamine in the solution, performing ultrasonic treatment to obtain uniformly mixed solution,wherein the mass ratio of neutral red, secondary water and triethylamine is (1-5):10000:(50-150); (2), transferring the mixed solution into a hydrothermal reactor, reacting for 8-10 hours at the temperature of 200-220 DEG C, standing after finishing the reaction, cooling to the room temperature, centrifuging to remove undissolved substance and extracting supernate, subjecting the supernate to dialysis through a 500-1000 Da dialysis bag in a glass container for at least three days, so as to obtain purified carbon dot aqueous solution; (3), freezing to dry the carbon dot aqueous solution to obtain carbon dot with orange-red fluorescence emission. The ratio type fluorescent carbon dot prepared by the method has single identification effect to Ag<+> and GSH, and its fluorescence color can bein reversible conversion between green and orange red; when used for Ag<+> and GSH detection, the carbon dot has good selectivity and high flexibility.

The invention discloses a tumor chemotherapy drug susceptibility detection kit which comprises a histocyte dispase, an antibiotic solution, an amphotericin solution, an EGTA-Trypsin (Ethylene Glycol Tetraacetic Acid) solution, a serum-containing culture medium DF10, a serum-free culture medium Kertin-SFM, a specimen preserving fluid, a specimen cleaning fluid, a gel coating culturing bottle, a cell fixing fluid, cell dispase, a cell filtering membrane, a neutral red dyeing solution and a collagen gel preparing reagent. A detection operation flow of the kit comprises the steps: primary cell culture, culture in collagen gel drops, and analysis of chemotherapy drug susceptibility. Compared with the prior art, the tumor chemotherapy drug susceptibility detection kit has the beneficial effects that the tumor chemotherapy drug susceptibility detection kit is capable of maximally protecting activity of tumor cells and shortening the drug susceptibility detection time. The tumor chemotherapy drug susceptibility detection kit contains all reagents required for tumor cell primary culture and chemotherapy drug detection; consumable items are disposable articles, thus bacterium pollution in an experiment process is reduced, and the convenience is brought for the use.

The alternative cellular energy (ACE) pathway provides a non-immunological defense mechanism against infectious diseases and can also function as an effective, non-scarring, tissue repair mechanism in response to injury. The ACE pathway can be activated using energy obtained from the ultraviolet light illumination of neutral red dissolved in alcohol. The effectiveness of the alcohol used in this procedure is significantly increased by first bubbling air containing “Water Gas” (otherwise referred to as Brown's Gas or HHO) through the alcohol. The Water Gas is considered as providing an additional charge to the alcohol. Water Gas can similarly be used to charge water and other liquids and to, thereby, increase the liquid's capacity to interact with various dyes, such as neutral red, and with other enerceuticals, which are regarded as being representative of the body's ACE pigments. Various uses are disclosed for using Water Gas charged alcohol and Water Gas charged water, for the therapeutic activation of the ACE pathway.

The invention relates to a method for measuring the content of titanium dioxide in titanium concentrate, and belongs to the field of analytical tests in titanium slag smelting. The method comprises the following steps: heating the titanium concentrate and strong phosphoric acid for reaction, isolating air in a medium of hydrochloric acid and sulfuric acid, reducing titanium (IV) to titanium (III) by aluminum, and in an oxalic acid environment, taking a neutral red solution as an indicator and titrating by a ferric ammonium sulfate standard solution until the solution is stably blue. According to the method, the acid dissolution process is carried out by strong phosphoric acid and an electrical furnace, and the operation is easy and quick; moreover, the operation time is short, five samples are simultaneously measured by the method, and the total measurement time is 1.5 hours; while the measurement time is 2.5 hours by a standard method of YB/T159.4-1999; the time is shortened by 40%.

The invention discloses a chromogenic medium used for detecting escherichia coli O157:H7. The medium contains agar, peptone, a yeast extract, sodium chloride, sorbitol, inositol, neutral red, a beta-galactosidase chromogenic substrate I 5-bromo-4-chloro-3-indolyl-beta-galactose, a beta-galactosidase chromogenic substrate II o-nitrophenyl-beta-D-galactoside, isopropyl-beta-D-thiopyran galactoside, bile salt, potassium tellurite and cefixime. The chromogenic medium is used for detecting escherichia coli O157:H7, has high detection sensitivity and good specificity and can be used for directly identifying strains according to colors of bacterial colonies. The color of the target bacterial colony escherichia coli O157:H7 is different from that of the prior art, thus providing more choices for researchers.

The kinetic energy of liquids can be increased by several methods described in the present application. The methods include exposure to a magnetic field provided by a rotating or vibrating magnet; exposure to an electromagnetic field provided by an electrical power cord; and placement of the liquid within the vicinity of electrostatic energy and/or sound energy. A previously described method is the bubbling of electrolysis-generated water gas (Brown's Gas) into the liquid. The increased kinetic energy can be demonstrated using a previously described neutral red dye kinetic assay (NR-Kinetic assay). Energized liquids include alcohol, alcoholic beverages and water. The use of the energized solutions to enhance the alternative cellular energy (ACE) pathway in the therapy of individuals is described.

The invention relates to a method used for detecting ostrea plicatula eosinophilic and basophilic granulocyte phagocytic ability, and the method comprises (1) ostrea plicatula blood cell suspension preparation; (2) fluorescent microsphere phagocytosis; (3) staining with neutral red; (4) lining dying with propidium iodide; and (5) microscopy. The method has the advantages of being simple in operation, rapid and accurate, strong in specificity, high in repeatability, and the like.