Tumor chemotherapy drug susceptibility detection kit

Abstract

The invention discloses a tumor chemotherapy drug susceptibility detection kit which comprises a histocyte dispase, an antibiotic solution, an amphotericin solution, an EGTA-Trypsin (Ethylene Glycol Tetraacetic Acid) solution, a serum-containing culture medium DF10, a serum-free culture medium Kertin-SFM, a specimen preserving fluid, a specimen cleaning fluid, a gel coating culturing bottle, a cell fixing fluid, cell dispase, a cell filtering membrane, a neutral red dyeing solution and a collagen gel preparing reagent. A detection operation flow of the kit comprises the steps: primary cell culture, culture in collagen gel drops, and analysis of chemotherapy drug susceptibility. Compared with the prior art, the tumor chemotherapy drug susceptibility detection kit has the beneficial effects that the tumor chemotherapy drug susceptibility detection kit is capable of maximally protecting activity of tumor cells and shortening the drug susceptibility detection time. The tumor chemotherapy drug susceptibility detection kit contains all reagents required for tumor cell primary culture and chemotherapy drug detection; consumable items are disposable articles, thus bacterium pollution in an experiment process is reduced, and the convenience is brought for the use.

Description

technical field [0001] The invention relates to the field of clinical detection, in particular to a tumor chemotherapeutic drug sensitivity detection kit. Background technique [0002] Drug resistance to chemotherapy is one of the main reasons for the failure of chemotherapy. How to formulate individualized chemotherapy regimens to improve the efficacy of chemotherapy is a clinical problem that needs to be solved urgently. A large number of clinical studies have shown that there is a certain correlation between in vitro drug sensitivity testing of tumors and clinical efficacy, and in vitro drug sensitivity testing can better guide clinical medication and improve clinical efficacy. [0003] At present, there are several methods for tumor drug sensitivity detection: soft agar colony formation assay (HTCA), tetrazolium blue colorimetry (MTT), differential staining cytotoxicity assay (DiSC), and adenosine triphosphate bioluminescence assay. However, these methods have different...

AI Technical Summary

Problems solved by technology

However, these methods have different degrees of deficiencies, which limit their clinical application. At present, there is an urgent need for practical and feasible detection methods for drug susceptibility testing.