Preparation method and use of white-rot fungus mycelium biological adsorbent

A biosorbent and white-rot fungus technology, applied in chemical instruments and methods, biological water/sewage treatment, adsorption water/sewage treatment, etc., can solve problems such as complicated operation, hindering light projection, and difficult to degrade, and achieve adsorption reaction The effect of mild conditions, easy industrial application, and simple preparation method

Inactive Publication Date: 2015-05-27
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The most direct pollution of dye wastewater to the environment is the pollution of its chroma. Even if the water body only contains dyes with a concentration of about 1mg / L, it can produce high chroma, which will not only affect the aesthetics of the water body, but also make the water body The light transmittance and gas solubility are reduced, thereby hindering the projection of light, causing the pollution of natural water bodies, the death of aquatic animals and plants, and the imbalance of the ecosystem, which directly or indirectly causes harm to the human body.
In addition, some synthetic dyes have certain toxicity and carcinogenic effects, due to the benzene ring structure, and one or more aromatic ring systems (OH-, NH 2 - or SH-) is connected through a conjugated system to form a chromophore, so it is difficult to be degraded, and its degradation products are mostly carcinogenic aromatic compounds such as benzidine. After being discharged into natural water bodies, they are enriched in water bodies. When the food chain is enriched and enters the human body, it can cause human malignant tumors, leading to malignant diseases such as bladder cancer, ureteral cancer, and renal pelvis cancer
[0003] At present, physical and chemical methods are mainly used to remove dye chroma, such as photooxidation, electrocoagulation, adsorption, reverse osmosis, membrane filtration, flocculation, ozone oxidation, deep injection, ion exchange, incineration and organic solvent extraction, etc. These methods have many disadvantages, such as high cost, complicated operation, refractory pollutants, and secondary pollution at the same time.

Method used

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  • Preparation method and use of white-rot fungus mycelium biological adsorbent
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  • Preparation method and use of white-rot fungus mycelium biological adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 prepares mycelia dry powder biosorbent

[0060] 1) Preparation of white rot fungus seed suspension

[0061] Inoculate the slightly acidic Poria perennialum refrigerated at 4°C on the plate medium, and carry out activation culture at 28±3°C for 7-8 days, and then inoculate an appropriate amount of the activated microacidic Poriaphyllum perennialis on the In 100mL of liquid medium, cultured on a shaker at a speed of 150rpm for 6 days at 28°C, then stirred with a homogenizer at a speed of 5000rpm for 30s to obtain a seed suspension;

[0062] Wherein, the preparation method of the liquid medium is as follows: glucose 20.0g, yeast extract powder 5.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O0.5g, ZnSO 4 ·7H 2 O50mg dissolved in deionized water, adjusted to a total volume of 1000mL, pH natural, 1×10 5 Pa autoclave for 30 minutes, add 10 mg of vitamin B1 that has been filtered and sterilized when finger temperature is touchable, mix well and set aside.

[0063] 2) P...

Embodiment 2

[0067] Embodiment 2 prepares mycelia dry powder biosorbent

[0068] In addition to the preparation method of the liquid medium described in step 1), glucose 22.0g, yeast extract powder 4.5g, KH 2 PO 4 1.1g, MgSO 4 ·7H 2 O0.45g, ZnSO 4 ·7H 2 O55mg dissolved in deionized water, adjust the total volume to 1000mL, pH natural, 1×10 5 Pa autoclave for 30 minutes, add 9 mg of vitamin B1 that has been filtered and sterilized when it is warm to the touch, and mix well for later use; in step 2), inoculate 2.2 mL of seed suspension into 100 mL of liquid medium at a temperature of 31 °C Under these conditions, culture was carried out on a shaker at a rotation speed of 160 rpm for 6 days; except that the drying temperature in step 3) was 80° C., the rest was the same as in Example 1.

Embodiment 3

[0069] Embodiment 3 prepares mycelia dry powder biosorbent

[0070] In addition to the preparation method of the liquid medium described in step 1), glucose 18.0g, yeast extract powder 5.5g, KH 2 PO 4 0.9g, MgSO 4 ·7H 2 O0.55g, ZnSO 4 ·7H 2 O45mg dissolved in deionized water, adjust the total volume to 1000mL, pH natural, 1×10 5 Pa autoclave for 30 minutes, add 11 mg of vitamin B1 that has been filtered and sterilized when it is warm to the touch, and mix well for later use; in step 2), inoculate 1.8 mL of seed suspension into 100 mL of liquid medium, at a temperature of 25 °C Under these conditions, culture was carried out on a shaker at a rotation speed of 140 rpm for 8 days; except that the drying temperature in step 3) was 70° C., the rest was the same as in Example 1.

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Abstract

The invention discloses a preparation method of a white-rot fungus mycelium biological adsorbent and a use of the white-rot fungus mycelium biological adsorbent in synthetic dye waste water. The preparation method comprises the following steps of inoculating a liquid medium with a white-rot fungus strain suspension, carrying out culture, acquiring mycelium, orderly carrying out centrifugation, washing and drying treatment, and then carrying out crushing or crushing modification treatment to obtain the white-rot fungus mycelium biological adsorbent. The preparation method has simple processes and a low cost. The white-rot fungus mycelium biological adsorbent is biodegradable and does not produce secondary pollution. A capacity of saturated adsorption of heterocyclic dye neutral red in heterocyclic dye neutral red model wastewater is 49.02mg / g or more, and the highest saturated adsorption capacity of the mycelium biological adsorbent subjected to aminated modification treatment is 91.74mg / g.

Description

technical field [0001] The invention belongs to the field of water treatment adsorbents, in particular to a preparation method of a white-rot fungus mycelium biosorbent and its application in treating synthetic dye wastewater. Background technique [0002] In recent years, synthetic dyes have been widely used in various industrial fields such as textiles, leather, pharmaceuticals, pulp and paper, plastics, carpets, printing, electroplating, and cosmetics manufacturing. With the rapid development of these industries and the continuous expansion of production scale, the amount of industrial wastewater discharged is increasing. According to statistics, the annual output of dyestuffs in the world has reached 8×10 6 t, where at least 10% of the dye enters natural water bodies. Due to the characteristics of large water volume, complex composition, large changes, poor biochemical properties, chromaticity, high chemical oxygen demand (COD) and biological oxygen demand (Biological o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C02F1/28
CPCB01J20/24C02F3/347C02F2101/308
Inventor 司静崔宝凯戴玉成
Owner BEIJING FORESTRY UNIVERSITY
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