Chromogenic medium used for detecting escherichia coli O157:H7

A chromogenic medium, O157 technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve problems such as experimental errors, and achieve the effect of avoiding non-systematic errors

Inactive Publication Date: 2015-03-04
SOOCHOW KH BIO SCI & TECH CO LTD KHB
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When strains are screened through multiple chromogenic media at the same time, there are experimental errors caused by the same or similar colo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chromogenic medium used for detecting escherichia coli O157:H7
  • Chromogenic medium used for detecting escherichia coli O157:H7
  • Chromogenic medium used for detecting escherichia coli O157:H7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red 、 β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.15g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β-D - 1 g of galactoside, 0.1 g of isopropyl-β-D-thiogalactopyranoside, 1.5 g of bovine bile salt, 2 mg of potassium tellurite, and 0.04 mg of cefixime.

Embodiment 2

[0035] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red , β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.2g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β-D - 1 g of galactoside, 0.1 g of isopropyl-β-D-thiogalactopyranoside, 1.5 g of bovine bile salt, 2 mg of potassium tellurite, and 0.04 mg of cefixime.

Embodiment 3

[0037] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red 、 β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.05g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β-D - 3 g of galactoside, 0.1 g of isopropyl-β-D-thiogalactopyranoside, 1.5 g of bovine bile salt, 2 mg of potassium tellurite, and 0.04 mg of cefixime.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a chromogenic medium used for detecting escherichia coli O157:H7. The medium contains agar, peptone, a yeast extract, sodium chloride, sorbitol, inositol, neutral red, a beta-galactosidase chromogenic substrate I 5-bromo-4-chloro-3-indolyl-beta-galactose, a beta-galactosidase chromogenic substrate II o-nitrophenyl-beta-D-galactoside, isopropyl-beta-D-thiopyran galactoside, bile salt, potassium tellurite and cefixime. The chromogenic medium is used for detecting escherichia coli O157:H7, has high detection sensitivity and good specificity and can be used for directly identifying strains according to colors of bacterial colonies. The color of the target bacterial colony escherichia coli O157:H7 is different from that of the prior art, thus providing more choices for researchers.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Escherichia coli O157:H7. Background technique [0002] Hemorrhagic Escherichia coli (enterohemorrhage E.Coli, EHEC) is a subtype of Escherichia coli, EHEC is divided into 157, 26, 111 serotypes, the main pathogenic strain is O157:H7, which can cause infectious diarrhea, because it can cause human named for hemorrhagic enteritis. [0003] Enterohaemorrhagic Escherichia coli O157:H7 is basically similar to Escherichia coli in other common characteristics except that it does not ferment or slowly ferment sorbitol and the encoded β-glucuronidase is inactive. Traditional slow fermentation sorbitol-MacConkey (SMAC) medium can be used as a medium for O157:H7 screening. On SMAC medium, O157:H7 colonies are colorless, while the fermented strains are pink, on Mérieux’s chromogenic medium, O157:H7 colonies are blue-green, on CHROMa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/04C12R1/19
Inventor 金京勋
Owner SOOCHOW KH BIO SCI & TECH CO LTD KHB
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap