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Chromogenic medium used for detecting escherichia coli O157:H7

A chromogenic medium, O157 technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve problems such as experimental errors, and achieve the effect of avoiding non-systematic errors

Inactive Publication Date: 2015-03-04
SOOCHOW KH BIO SCI & TECH CO LTD KHB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When strains are screened through multiple chromogenic media at the same time, there are experimental errors caused by the same or similar colo

Method used

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  • Chromogenic medium used for detecting escherichia coli O157:H7
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  • Chromogenic medium used for detecting escherichia coli O157:H7

Examples

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Effect test

Embodiment 1

[0033] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red 、 β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.15g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β-D - 1 g of galactoside, 0.1 g of isopropyl-β-D-thiogalactopyranoside, 1.5 g of bovine bile salt, 2 mg of potassium tellurite, and 0.04 mg of cefixime.

Embodiment 2

[0035] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red , β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.2g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β-D - 1 g of galactoside, 0.1 g of isopropyl-β-D-thiogalactopyranoside, 1.5 g of bovine bile salt, 2 mg of potassium tellurite, and 0.04 mg of cefixime.

Embodiment 3

[0037] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red 、 β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.05g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β-D - 3 g of galactoside, 0.1 g of isopropyl-β-D-thiogalactopyranoside, 1.5 g of bovine bile salt, 2 mg of potassium tellurite, and 0.04 mg of cefixime.

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Abstract

The invention discloses a chromogenic medium used for detecting escherichia coli O157:H7. The medium contains agar, peptone, a yeast extract, sodium chloride, sorbitol, inositol, neutral red, a beta-galactosidase chromogenic substrate I 5-bromo-4-chloro-3-indolyl-beta-galactose, a beta-galactosidase chromogenic substrate II o-nitrophenyl-beta-D-galactoside, isopropyl-beta-D-thiopyran galactoside, bile salt, potassium tellurite and cefixime. The chromogenic medium is used for detecting escherichia coli O157:H7, has high detection sensitivity and good specificity and can be used for directly identifying strains according to colors of bacterial colonies. The color of the target bacterial colony escherichia coli O157:H7 is different from that of the prior art, thus providing more choices for researchers.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Escherichia coli O157:H7. Background technique [0002] Hemorrhagic Escherichia coli (enterohemorrhage E.Coli, EHEC) is a subtype of Escherichia coli, EHEC is divided into 157, 26, 111 serotypes, the main pathogenic strain is O157:H7, which can cause infectious diarrhea, because it can cause human named for hemorrhagic enteritis. [0003] Enterohaemorrhagic Escherichia coli O157:H7 is basically similar to Escherichia coli in other common characteristics except that it does not ferment or slowly ferment sorbitol and the encoded β-glucuronidase is inactive. Traditional slow fermentation sorbitol-MacConkey (SMAC) medium can be used as a medium for O157:H7 screening. On SMAC medium, O157:H7 colonies are colorless, while the fermented strains are pink, on Mérieux’s chromogenic medium, O157:H7 colonies are blue-green, on CHROMa...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/19
Inventor 金京勋
Owner SOOCHOW KH BIO SCI & TECH CO LTD KHB
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