Color-developing culture medium for detecting escherichia coli O157:H7

A chromogenic medium and O157 technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as experimental errors, and achieve the effect of avoiding non-systematic errors

Inactive Publication Date: 2018-02-23
黄增才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When strains are screened through multiple chromogenic media at the same time, there are experimental errors caused by the same or similar color development, so it is necessary to develop chromogenic media with different color development schemes to meet the needs of scientific research

Method used

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  • Color-developing culture medium for detecting escherichia coli O157:H7

Examples

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Effect test

Embodiment 1

[0033] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red , β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.15g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β- D-galactoside 1g, isopropyl-β-D-thiogalactopyranoside 0.1g, bovine bile salt 1.5g, potassium tellurite 2mg, cefixime 0.04mg.

Embodiment 2

[0035] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red , β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.2g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β- D-galactoside 1g, isopropyl-β-D-thiogalactopyranoside 0.1g, bovine bile salt 1.5g, potassium tellurite 2mg, cefixime 0.04mg.

Embodiment 3

[0037] A chromogenic medium for detecting Escherichia coli O157:H7, each 1000mL medium contains 15g of agar, 10g of peptone, 5g of yeast extract, 5g of sodium chloride, 15g of sorbitol, 5g of inositol, and 0.01g of neutral red , β-galactosidase chromogenic substrate Ⅰ 5-bromo-4-chloro-3-indole-β-galactose 0.05g, β-galactosidase chromogenic substrate Ⅱ o-nitrobenzene-β- D-galactoside 3g, isopropyl-β-D-thiogalactopyranoside 0.1g, bovine bile salt 1.5g, potassium tellurite 2mg, cefixime 0.04mg.

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Abstract

The invention discloses a chromogenic medium for detecting Escherichia coli O157:H7, the medium contains agar, peptone, yeast extract, sodium chloride, and also contains sorbitol, inositol, neutral red, β-semi Lactosidase chromogenic substrate Ⅰ 5‑bromo‑4‑chloro‑3‑indole‑β‑galactose, β‑galactosidase chromogenic substrate Ⅱ o-nitrobenzene‑β‑D‑galactoside, iso Propyl‑β‑D‑thiogalactopyranoside, ox bile salt, potassium tellurite, cefixime. The chromogenic medium of the present invention is used to detect Escherichia coli O157:H7, has high detection sensitivity and good specificity, and can directly identify bacterial strains according to the color of the colony, and the color of the target colony Escherichia coli O157:H7 is different from the prior art. Researchers offer more options.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Escherichia coli O157:H7. Background technique [0002] Hemorrhagic Escherichia coli (enterohemorrhage E. Coli, EHEC) is a subtype of Escherichia coli, EHEC is divided into 157, 26, 111 serotypes, the main pathogenic strain is O157:H7, which can cause infectious diarrhea, because it can cause human named for hemorrhagic enteritis. [0003] Enterohaemorrhagic Escherichia coli O157:H7 is basically similar to Escherichia coli except that it does not ferment or slowly ferment sorbitol and the encoded β-glucuronidase is inactive. Traditional slow fermentation sorbitol-MacConkey (SMAC) medium can be used as a medium for O157:H7 screening. On SMAC medium, O157:H7 colonies are colorless, while the fermented strains are pink, on Mérieux’s chromogenic medium, O157:H7 colonies are blue-green, on CHROMagar’s chromogenic medium, O...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10
CPCC12Q1/10C12Q2334/52
Inventor 黄增才
Owner 黄增才
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