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Method for fermented production of succinic acid on the basis of electrochemical system for regulating intracellular reducing power regeneration

A succinic acid production, electrochemical technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of differences in electrochemical regulation methods of reducing power, achieve great social significance and economic value, improve yield effect

Active Publication Date: 2016-04-13
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the reducing power required by different metabolites and the required electrochemical regulation methods are different.

Method used

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  • Method for fermented production of succinic acid on the basis of electrochemical system for regulating intracellular reducing power regeneration
  • Method for fermented production of succinic acid on the basis of electrochemical system for regulating intracellular reducing power regeneration
  • Method for fermented production of succinic acid on the basis of electrochemical system for regulating intracellular reducing power regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example illustrates the anaerobic fermentation of Escherichia coli AFP111.

[0044] Escherichia coli (Escherichiacoli) AFP111 anaerobic fermentation method is as follows:

[0045] The Escherichia coli (Escherichiacoli) AFP111 in the cryopreservation tube was used as the starting strain, inoculated with 1% (v / v) inoculum in a test tube containing 5 mL of seed medium, and cultivated at 37°C and 200r / min for 12h Obtain the primary seed liquid; inoculate the primary seed liquid into a 500mL Erlenmeyer flask containing 100mL seed medium at an inoculum volume of 1% (v / v), and incubate at 37°C and 200r / min for 6h to obtain the secondary seed liquid . The secondary seed solution was inoculated into a 700mL chemostat device containing 450mL fermentation medium at an inoculum volume of 10% (v / v). At the same time, sterile carbon dioxide was continuously passed into the chemostat to maintain anaerobic conditions. surroundings. Periodically aseptic sampling during the ferme...

Embodiment 2

[0051] This example illustrates a one-step electrochemical anaerobic fermentation method using Escherichia coli (Escherichiacoli) AFP111 under the condition of -0.65V and the addition of 0.05mmol / L neutral red.

[0052] The one-step electrochemical anaerobic fermentation method of Escherichia coli (Escherichiacoli) AFP111 is as follows:

[0053]The Escherichia coli (Escherichiacoli) AFP111 in the cryopreservation tube was used as the starting strain, inoculated with 1% (v / v) inoculum in a test tube containing 5 mL of seed medium, and cultivated at 37°C and 200r / min for 12h Obtain the primary seed liquid; inoculate the primary seed liquid into a 500mL Erlenmeyer flask containing 100mL seed medium at an inoculum volume of 1% (v / v), and incubate at 37°C and 200r / min for 6h to obtain the secondary seed liquid . The secondary seed solution was inoculated into a 700mL cathode chamber containing 450mL of fermentation medium at an inoculum size of 10% (v / v). At the same time, a volta...

Embodiment 3

[0059] This example illustrates a one-step electrochemical anaerobic fermentation method using Escherichia coli (Escherichiacoli) AFP111 under the condition of -0.65V and the addition of 0.1mmol / L riboflavin.

[0060] The one-step electrochemical anaerobic fermentation method of Escherichia coli (Escherichiacoli) AFP111 is as follows:

[0061] The Escherichia coli (Escherichiacoli) AFP111 in the cryopreservation tube was used as the starting strain, inoculated with 1% (v / v) inoculum in a test tube containing 5 mL of seed medium, and cultivated at 37°C and 200r / min for 12h Obtain the primary seed liquid; inoculate the primary seed liquid into a 500mL Erlenmeyer flask containing 100mL seed medium at an inoculum volume of 1% (v / v), and incubate at 37°C and 200r / min for 6h to obtain the secondary seed liquid . The secondary seed solution was inoculated into a 700mL cathode chamber containing 450mL of fermentation medium at an inoculation amount of 10% (v / v). At the same time, a v...

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Abstract

The invention discloses a method for fermented production of succinic acid on the basis of an electrochemical system for regulating intracellular reducing power regeneration. The method comprises three steps including strain activation, seed culture and anaerobic fermentation production of succinic acid, anaerobic fermentation adopts electrochemical fermentation, an electron carrier is added to a fermentation medium, the corresponding voltage is applied to a cathode, and the concentration of the electron carrier is in a range of 0.01-0.5 mmol / L. the electron carrier is neutral red or riboflavin. The cathode voltage applied to the cathode is scanned and determined in the range from 0.1 V to 1 V with the cyclic voltammetry at the scanning speed of 5-10 mV / s. When fermentation is performed in an electrochemical device under the anaerobic condition, the total amount of intracellular NADH is increased under the assistance of the electron carrier, the intracellular reducing power (NADH / NAD+) level is tripled, the succinic acid accumulation reaches 15.06 g / L, and efficient synthesis of the reduced product, namely, the succinic acid, is facilitated.

Description

technical field [0001] The invention relates to a method for producing succinic acid through regeneration and fermentation of intracellular reducing power regulation based on an electrochemical system, and belongs to the technical field of biochemical industry. Background technique [0002] For different carbon sources and products, the supply and consumption levels of reducing power are not always consistent. For example, when 1 molecule of glucose and 1 molecule of glycerol are converted into phosphoenolpyruvate through the glycolytic pathway, 2 molecules of NADH are produced, If the final product is succinic acid or ethanol with strong reducibility, when glucose is the only carbon source, the reducing power used for product synthesis is obviously insufficient, which reduces the yield of the reducing product, while glycerol is the carbon source. , and because of excess reducing power, the growth of bacteria stagnates. In order to balance the intracellular coenzyme metabol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/19
CPCC12P7/46
Inventor 姜岷冀亚亮马江锋吴明科吴昊张敏
Owner NANJING UNIV OF TECH
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