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Immobilized biological adsorbent, its preparation method and application

A biosorbent and adsorbent technology, applied to chemical instruments and methods, fixed on or in inorganic carriers, alkali metal compounds, etc., can solve the problem that it is difficult to find and effectively remove pollutants at the same time, so as to improve the treatment effect and adsorption capacity, enhanced adsorption effect, special structure effect

Inactive Publication Date: 2012-04-25
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are both heavy metal ions and organic dyes in printing and dyeing wastewater. Due to the differences in structure and properties of heavy metal ions and organic dyes, it is difficult to find a method that can effectively remove the two pollutants at the same time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] An immobilized biological adsorbent of the present invention, the adsorbent includes Phanerochaete chrysosporium, and the surface of Phanerochaete chrysosporium is evenly coated with a layer of Fe 3 O 4 Nano particles, Fe 3 O 4 Nanoparticles are used as carriers to fix Phanerochaete chrysosporium.

[0025] The method for preparing the above-mentioned immobilized biosorbent includes the following steps:

[0026] 1. Preparation of Fe 3 O 4 Nanoparticles

[0027] (1) Prepare acidic iron salt solution: Weigh 4.16g FeCl 3 ·6H 2 O and 1.6g FeCl 2 ·4H 2 O, mixed with 0.68ml of HCl with a concentration of 12mol / L, in which Fe 3+ , Fe 2+ The molar ratio with HCl is 3:1:1, then add the mixed solution to 20ml of ultrapure water (fill the water with nitrogen for 10min before use), stir evenly to obtain an acidic iron salt solution;

[0028] (2) Generate Fe 3 O 4 Suspension: Add the above acidic iron salt solution dropwise to 120ml NaOH solution with a concentration of 1.5mol / L, stir for 30m...

Embodiment 2

[0039] A preparation method of the immobilized biosorbent of the present invention includes the following steps:

[0040] 1. Fe 3 O 4 The preparation of nanoparticles is the same as in Example 1;

[0041] 2. The preparation of the spore suspension of Phanerochaete chrysosporium is the same as in Example 1;

[0042] 3. Fe 3 O 4 Phanerochaete chrysosporium immobilized by nanoparticles

[0043] Weigh the Fe prepared in step 1 above 3 O 4 Add 0.2g of nanoparticles to 100 ml of Phanerochaete chrysosporium culture medium (pH 5.5), sterilize at 115°C for 30 minutes, and inoculate 2 ml of spore suspension (the number of spores is 4.0×10 6 A), then culturing at a constant temperature and shaking at 150 rpm for 4 to 5 days at a culture temperature of 35°C to 37°C. The bacteria balls are filtered out and washed with ultrapure water 3 times for use.

[0044] Add the adsorbent prepared above to 50ml neutral red and Pb according to 1.5g wet weight 2+ In the simulated dye wastewater with a concentrati...

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Abstract

The invention discloses an immobilized biological adsorbent containing phanerochete chrysosporium, the surface of which is uniformly wrapped by a layer of carrier Fe3O4 nanoparticles for fixation. The method comprises the steps of: first adding Fe3O4 nanoparticles into a phanerochete chrysosporium liquid medium containing tween 80, then inoculating a phanerochete chrysosporium spore suspension, conducting shaking cultivation so as to obtain the immobilized biological adsorbent. The invention also discloses application of the adsorbent in dye wastewater treatment. The biological adsorbent of the invention has high adsorption efficiency and large adsorption capacity, and can be effectively used for treating neutral red and lead in dye wastewater. The preparation technology and treatment technology in the invention are characterized by simplicity and low cost.

Description

Technical field [0001] The invention involves the field of dye wastewater treatment, and specifically involves a biological adsorption agent and its preparation methods and applications. Background technique [0002] Dye wastewater is a large -scale, wide -moving industrial wastewater, which causes serious pollution to the ecological environment.The ingredients of wastewater in the dye industry are very complicated. It not only contains dyes, intermediates and various auxiliary materials, but also contain heavy metals and inorganic salts such as mercury, cadmium, copper, chromium.In addition, dye wastewater often contains difficulty degradation and toxic organic pollutants. Some even organic matter that causes cancer, mutations, and terators cause great harm to the ecological environment and drinking water.one.As the new artificial synthetic dyes continue to occur, the problem of dye wastewater treatment has become more complicated and urgent. [0003] Neutral red is one of the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30C12N11/14C02F3/34
Inventor 曾光明许飘曾茁桐黄丹莲冯冲凌赖萃赵美花危臻黄超赵伟伟
Owner HUNAN UNIV
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