Method for detecting cysteine by using neutral red-gold composite material modified electrode

A technology of composite material and cysteine, which is applied in the field of neutral red-gold composite material modified electrode to detect cysteine, can solve the problems of complex pretreatment and high cost, and achieve simple preparation process, low cost, good The effect of applying the foreground

Inactive Publication Date: 2017-11-21
GUILIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

The above-mentioned main instrument detection methods have the advantage

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  • Method for detecting cysteine by using neutral red-gold composite material modified electrode
  • Method for detecting cysteine by using neutral red-gold composite material modified electrode
  • Method for detecting cysteine by using neutral red-gold composite material modified electrode

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Embodiment

[0017] (1) Grind the glassy carbon electrode into a mirror surface with alumina powder, and then use 1:1 HNO 3 、1:1CH 3 CH 2 OH and H 2 O was ultrasonically cleaned for 15 minutes, taken out and washed, put into 10 mL of potassium ferricyanide solution, connected to the three-electrode system, carried out cyclic voltammetry scanning at -0.4~0.6 V, and activated the glassy carbon electrode until the cycle The voltammetric curve is stable, and the peak potential difference is less than 90mV. The obtained activated glassy carbon electrode is cleaned and immersed in secondary water for later use.

[0018] (2) Grind the gold electrode into a mirror surface with alumina powder, and then use 1:1 HNO 3 、1:1CH 3 CH 2 OH and H 2 O was ultrasonically cleaned for 15 minutes, taken out and cleaned, put into 10 mL of sulfuric acid solution with a concentration of 1 mol / L, connected to the three-electrode system, and carried out cyclic voltammetry scanning at -0.4~0.6 V to activate the...

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Abstract

The invention discloses a method for detecting cysteine by using a neutral red-gold composite material modified electrode. The method comprises: coating the surface of a glassy carbon electrode with the mixed solution of neutral red and chloroauric acid in a dropwise manner, naturally drying to form a film layer on the surface of the glassy carbon electrode so as to prepare the neutral red-gold composite material modified glassy carbon electrode, and detecting cysteine in a PBS buffer solution (pH value of 7) by using the neutral red-gold composite material modified glassy carbon electrode through a differential pulse voltammetry method, wherein the linear relationship between the electrode reduction peak current decreasing degree and the cysteine concentration is presented within a certain range, the linear range is 5*10<-6>-1*10<-4> mol/L, r is 0.998, and the detection limit is 2*10<-6> mol/L. According to the present invention, the preparation process of the modified electrode is simple, the cost is low, and the good application prospect is provided in the detection of cysteine.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection, in particular to a method for detecting cysteine ​​with a neutral red-gold composite material modified electrode. Background technique [0002] Cysteine ​​is a common amino acid and the basic substance of protein, which is involved in many physiological processes. Medical research shows that the change of cysteine ​​content has an important relationship with many diseases, such as liver damage, skin damage, etc., which can be used as the basis for the diagnosis of these diseases. Therefore, real-time, accurate, rapid and highly selective detection of cysteine ​​content in blood samples and cells is of great significance for the diagnosis of many diseases. Currently, there are many commonly used detection methods for cysteine, such as high performance liquid chromatography and capillary electrophoresis. The above-mentioned main instrumental detection methods have t...

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Application Information

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IPC IPC(8): G01N27/30G01N27/333
CPCG01N27/30G01N27/333
Inventor 潘宏程庞光明薛争争
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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