Preparation method and application of ratio type fluorescent carbon dot for detecting sertraline and glutathione

Abstract

The invention discloses a preparation method and application of a ratio type fluorescent carbon dot for detecting sertraline and glutathione, belongs to the field of fluorescent carbon dots, and aimsto provide a preparation method and application of a ratio type fluorescent carbon dot with a specific recognition effect on sertraline and glutathione. The method comprises following steps: dissolving neutral red and polyethyleneimine in secondary water, carrying out ultrasonic treatment to obtain a uniform mixed solution, transferring the uniform mixed solution into a hydrothermal reaction kettle, reacting for 4-8 hours at the temperature of 150-200 DEG C, standing and cooling the reaction product to room temperature after the reaction is stopped, centrifuging the liquid to remove insolublesubstances, collecting a supernatant, and carrying out dialysis treatment in a glass container for at least three days by virtue of a dialysis bag being 500-1000 Da, so as to obtain a pure carbon dotaqueous solution; and performing freeze drying to obtain the orange red fluorescence emission carbon dots. The prepared ratio type fluorescent carbon dot has a specific recognition effect on sertraline and glutathione, wherein the fluorescence of the carbon dot shows orange-green-orange reversible transformation, and the ratio type fluorescent carbon dot is used for detecting sertraline and glutathione and is good in selectivity and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of fluorescent carbon dots, and in particular relates to a preparation method and application of ratio-type fluorescent carbon dots for detecting sertraline and glutathione. Background technique [0002] Sertraline is a potent and selective inhibitor of neuronal serotonin reuptake. At clinical doses, sertraline blocks the uptake of serotonin by human platelets and is only sensitive to norepinephrine and dopamine. weak impact. In vitro studies showed that sertraline had no significant effect on adrenergic receptors, cholinergic receptors, GABA receptors, dopaminergic receptors, histamine receptors, serotonergic receptors (or benzodiazepine receptors). Affinity. The antagonism of the above receptors is considered to be related to the sedative effect, anticholinergic effect and cardiotoxicity of other psychiatric drugs. Sertraline has no inhibitory effect on monoamine oxidase. The plasma protein binding rate o...

AI Technical Summary

Problems solved by technology

Most of the currently synthesized carbon dots emit blue-green fluorescence, which limits their applications in biomedical and optoelectronic devices

A few literatures have reported the application of long-wave emission of carbon dots, but these long-wave fluorescent carbon dots only have a single emission peak and the intensity change of a single emission peak, and are easily affected by other concentrations and external environmental conditions.

Images