Tissue engineered esophagus

A technology of tissue engineering and esophagus, applied in the field of biomedical engineering, can solve problems such as slow progress and achieve the effect of accelerating blood supply

Inactive Publication Date: 2003-04-16
谭强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development of artificial esophagus is progressing slowly due to the absence of synthetic materials with good histocompatibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 11

[0011] Example 11) Isolation and cultivation of epidermal keratinocytes

[0012] The patient's epidermis was trimmed into strips with a width of 1-2mm, rinsed twice in PBS without calcium and magnesium, and immersed in 0.5% dispase II overnight at 4°C with the epidermis facing upward. After the epidermis was torn off from the dermis, it was cut into pieces and digested with 0.05% trypsin-0.53mM EDTA at 37°C for 20 minutes, and 10ml of trypsin inhibitor was used to terminate the digestion. Filter through a 150-mesh stainless steel filter, centrifuge (1200 rpm, 10 minutes), and count to make a single-cell suspension, press 3×106 / 75cm 2 Density seeding culture, serum-free epidermal cell medium (GIBRO, USA), plus bovine pituitary extract and recombinant epidermal growth factor, at 37°C, 5% CO 2 , cultured in an incubator with saturated humidity, and the culture medium was changed every 3 days. When the cells reached 60-75% confluence, they were digested with 0.05% trypsin at 37°...

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PUM

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Abstract

A histo-enigineered esophagus is disclosed, and it contains a dual-layer structure of epithelial layer and dermis layer constructed by scaffold material and seed cells. The vascular endothelial cellsin the dermis layer directly take part in the creation of capillary network. Two revascularization methods are used for quickly communicating with the blood supply channel of receptor.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a tissue-engineered esophagus and a preparation method thereof. Background technique [0002] my country is a country with a high incidence of esophageal cancer and the highest mortality rate of esophageal cancer. The current clinical treatment principles are generally transthoracic resection of esophageal cancer and one-stage reconstruction of the digestive tract. The most common esophagus substitutes are the stomach, colon, and small intestine. The operation performed is traumatic, involves a wide range, and has many complications. The incidence of anastomotic leakage is about 3% to 5%. It is a typical treatment mode of repairing trauma by trauma. The development of artificial esophagus is progressing slowly due to the absence of synthetic materials with good histocompatibility. [0003] Tissue engineering is a hot spot in life science in the 21st century. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/04A61L27/00
Inventor 谭强
Owner 谭强
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