Method for preparing bacterial cellulose gel composite material

A technology of bacterial cellulose and composite materials, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., which can solve the problems of material waste and low compounding rate

Active Publication Date: 2013-04-10
SHENZHEN INST OF ADVANCED TECH
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AI Technical Summary

Problems solved by technology

[0003] However, when using in-situ compounding to prepare composite materials of bacterial cellulose and other organic macromolecules, since this combination is a physical combination, the macromolecular materials dispersed in the culture medium cannot enter the network of bacterial cellulose very well. The space of the shape film, the recombination rate is low, resulting in the waste of materials

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  • Method for preparing bacterial cellulose gel composite material
  • Method for preparing bacterial cellulose gel composite material
  • Method for preparing bacterial cellulose gel composite material

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preparation example Construction

[0026] see figure 1 , the preparation method of the spherical bacterial cellulose gel composite material of one embodiment, comprising:

[0027] S101. Prepare a culture solution containing glucose, peptone, anhydrous disodium hydrogen phosphate and citric acid.

[0028] The culture medium is a nutrient for the growth and maintenance of bacteria, and promotes the formation of products. In this embodiment, the culture solution may include glucose, peptone, anhydrous disodium hydrogen phosphate and citric acid. Dextrose, peptone, anhydrous disodium hydrogen phosphate and citric acid. The specific operation can be as follows: prepare 100mL culture medium. Mix 20 g of glucose, 5 g of peptone, 2.7 g of anhydrous disodium hydrogen phosphate, and 1.15 g of citric acid. Adjust the pH value of the culture solution to 6.0, which is suitable for the growth of bacteria. Then other components can be added to the culture solution according to the needs of different bacterial species for...

Embodiment 1

[0043] Preparation of culture medium: 100 mL of culture liquid was composed of 20 g of glucose, 5 g of peptone, 2.7 g of anhydrous disodium hydrogen phosphate, 1.15 g of citric acid, 1 g of magnesium sulfate, 1 g of ammonium sulfate, and 1 mL of corn syrup extract.

[0044] Preparation of bacterial inoculum: Take 1 mL of JCM 9730 Acetobacter xylinum bacteria liquid and place it in 100 mL of culture liquid. After static culture at 30°C for 2 days, transfer the culture liquid and the resulting bacterial cellulose film to a sterile After breaking up the bacterial cellulose film in a blender, add 2mL of cellulase aqueous solution and enzymolyze it at 30°C for 2 hours. Minutes, take the precipitate as the bacterial inoculum.

[0045] Preparation of bacterial solution: Mix the above-mentioned bacterial inoculum with physiological saline at a ratio of 1:1 by volume and set aside.

[0046] Preparation of sterile gel: Take 25g of chitosan with a molecular weight ranging from 10,000 to...

Embodiment 2

[0050] 1. Preparation of culture medium: 100 mL of culture liquid was composed of 20 g of glucose, 5 g of peptone, 2.7 g of anhydrous disodium hydrogen phosphate, 1.15 g of citric acid, 1 g of magnesium sulfate, 1 g of ammonium sulfate, and 1 mL of corn syrup extract.

[0051] 2. Preparation of bacterial inoculum: Take 1 mL of JCM 9730 Acetobacter xylinum bacteria liquid and place it in 100 mL of culture liquid. After static culture at 30°C for 2 days, transfer the culture liquid and the resulting bacterial cellulose film to In a sterilized mixer, break up the bacterial cellulose film, add 1mL of cellulase aqueous solution for every 100mL of cellulosic bacteria solution, and enzymatically hydrolyze at 33°C for 1 hour. The centrifuge uses a centrifugal force of 3000RCF, centrifuges for 10 minutes, and takes the precipitate as the bacterial inoculum.

[0052] 3. Preparation of bacterial solution: Mix the above-mentioned bacterial inoculum with normal saline according to the volu...

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Abstract

The invention relates to a method for preparing a bacterial cellulose gel composite material, which comprises the following steps: preparing culture solution; inoculating a strain of acetobacter xylinum into the culture solution, carrying out static culture for 2 days at a temperature of 30 DEG C to generate a bacterial cellulose thin film, dispersing the bacterial cellulose thin film under the aseptic condition, and centrifuging after carrying out enzymolysis on the bacterial cellulose thin film so as to obtain bacterial inoculated solution; preparing bacteria solution from the bacterial inoculated solution and normal saline according to a volume ratio of 1:1 to 1:3; after preprocessing a composite ligand, sterilizing the composite ligand to obtain aseptic gel; sufficiently mixing the aseptic gel and the bacteria solution according to a volume ratio of 1:1 to 3:1, dropwise adding the mixture into first aseptic calcium chloride solution to form gel complex globule, then transferring the gel complex globule into second aseptic calcium chloride solution to carry out standing for 6 to 12 hours, placing the obtained product into newly prepared culture solution and carrying out rotating fermentation for 2 to 3 days so as to obtain a bacterial cellulose gel composite base material; and carrying out rotating fermentation or static fermentation on the bacterial cellulose gel composite base material for 2 to 5 days so as to obtain the ball-like or film-shaped bacterial cellulose gel composite material. The method is high in composite rate and in material utilization rate.

Description

technical field [0001] The invention belongs to the field of biological composite materials, in particular to a method for preparing bacterial cellulose gel composite materials. Background technique [0002] Gluconacetobacter xylinum can synthesize extracellular cellulose that is secreted into the culture medium. The secreted bacterial cellulose exists in the form of a hydrated gel, and its water content is 500 to 700 times its dry weight. Therefore, in medicine, this high-moisture material is very suitable as a directly utilized material. Hydrated glue material for wound repair. Bacterial cellulose has the same molecular and chemical composition as plant-derived cellulose, but bacterial cellulose has high purity, high crystallinity, nanostructure, skin-like mechanical properties in the hydrated state and easy passage through various It has the advantages of a variety of utensils and molds to shape the shape. As an independent material, bacterial cellulose has been used i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/38A61K47/36A61K47/42A61K9/06A61K31/722A61K31/717A61P17/02A61P43/00A61L31/12A61L31/04A61L31/16
Inventor 胡阳高楠莎潘浩波吕小桥
Owner SHENZHEN INST OF ADVANCED TECH
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