Preparation method of graphene/bacterial cellulose composite material

Abstract

The invention discloses a preparation method of a graphene / bacterial cellulose composite material. Bacterial cellulose and graphene are co-cultured in situ to obtain the graphene / bacterial cellulose composite material, wherein the graphene evenly grows in the bacterial cellulose reticular fiber structure. The preparation method comprises the following steps: 1) preparing a bacterial cellulose culture fluid, and sterilizing at high temperature under high pressure for 30-60 minutes; 2) inoculating the strain in the bacterial cellulose culture fluid, and rocking in a rocker for 12-48 hours; 3) carrying out ultrasonic treatment on a 0.2mg / ml graphene dispersion liquid for 1-3 hours, and adding the graphene dispersion liquid into the bacterial cellulose culture fluid with the strain, wherein the volume ratio of the graphene dispersion liquid to the bacterial cellulose culture fluid is 1:5-1:10; 4) rocking the mixed liquid in the rocker for 12-24 hours; 5) putting the liquid in a 28 DEG C thermostatic oven, and standing for 1-2 weeks to obtain the graphene / bacterial cellulose composite material; and 6) cleaning the graphene / bacterial cellulose composite material, and carrying out freeze-drying. In the composite material, the graphene is evenly distributed on the bacterial cellulose fibers, thereby effectively inhibiting the defect of high agglomeration tendency of graphene particles.

Description

technical field

[0001] The invention relates to a composite material, in particular to a method for preparing a composite material composed of graphene and bacterial cellulose. Background technique

[0002] Bacterial cellulose is a general term for cellulose synthesized by microorganisms. Among them, the typical one is Acetobacter xylinum in the genus Acetobacter, which has the highest cellulose production capacity and is recognized as a model strain for studying cellulose synthesis, crystallization process and structural properties. Compared with plant cellulose, bacterial cellulose has many unique properties: ① It has high chemical purity and high crystallinity; ② It has strong water holding capacity, and the water holding capacity of undried bacterial cellulose can reach more than 1000%; ③It has high biocompatibility and biodegradability; ④The fiber diameter is between 0.01~0.1μm, the elastic modulus is several times to ten times that of ordinary plant fibers, and the te...

Examples

Embodiment 1

[0034] First, use pure water, glucose, peptone, citric acid, disodium hydrogen phosphate, potassium dihydrogen phosphate, and yeast powder to prepare a bacterial cellulose fermentation culture solution. The pH of the culture solution is 5.0, and the bacterial cellulose fermentation culture solution is placed in In a sterilizing pot, sterilize at 120°C and 0.1MPa for 30 minutes; then, insert the activated Acetobacter xylinum species into the above-mentioned bacterial cellulose culture solution, and cultivate it on a shaking table for 12 hours; the mass volume concentration is 0.2mg / ml of graphene dispersion, ultrasonically dispersed for 1 hour, and sterilized by ultraviolet light three times, each time for 15 minutes; In the bacterial cellulose culture solution of bacterial strain; Shake the above-mentioned mixed liquid shaker for 12 hours to make the mixed liquid uniform; put the incubator at 28° C. for static cultivation for 1 week, and obtain the graphene / bacterial cellulose...

Embodiment 2

[0037] The difference between the preparation process of this embodiment 2 and the above-mentioned embodiment 1 is that, wherein, the graphene dispersion is added to the bacteria-carrying In the bacterial cellulose culture solution of species; shake the above-mentioned mixed liquid shaker for 12 hours to make the mixed liquid uniform.

[0038] figure 1 (c) and figure 1 (d) are respectively the SEM photos of the graphene / bacterial cellulose composite material of Example 2; figure 2 (b) is a TEM photo of the graphene / bacterial cellulose composite material of Example 2; FIG. 3(b) is a Raman spectrum test curve of the graphene / bacterial cellulose composite material of Example 2.

Embodiment 3

[0040]The only difference between the present embodiment 3 and the preparation process of the above-mentioned embodiment 1 is that the activated Acetobacter xylinum species is inserted into the above-mentioned bacterial cellulose culture solution, and cultured on a shaking table for 24 hours; the mass volume concentration is 0.2 mg / ml graphene dispersion, ultrasonically dispersed for 2 hours; shake the mixed liquid on a shaker for 24 hours to make the mixed liquid uniform; put it in an incubator at 28°C for static culture for 2 weeks.

[0041] figure 1 (e) and figure 1 (f) SEM photos of the graphene / bacterial cellulose composite material of Example 3; figure 2 (c) is the TEM photo of the graphene / bacterial cellulose composite material of Example 3.

technical field

[0001] The invention relates to a composite material, in particular to a method for preparing a composite material composed of graphene and bacterial cellulose. Background technique

[0002] Bacterial cellulose is a general term for cellulose synthesized by microorganisms. Among them, the typical one is Acetobacter xylinum in the genus Acetobacter, which has the highest cellulose production capacity and is recognized as a model strain for studying cellulose synthesis, crystallization process and structural properties. Compared with plant cellulose, bacterial cellulose has many unique properties: ① It has high chemical purity and high crystallinity; ② It has strong water holding capacity, and the water holding capacity of undried bacterial cellulose can reach more than 1000%; ③It has high biocompatibility and biodegradability; ④The fiber diameter is between 0.01~0.1μm, the elastic modulus is several times to ten times that of ordinary plant fibers, and the te...

Examples

Embodiment 1

[0034] First, use pure water, glucose, peptone, citric acid, disodium hydrogen phosphate, potassium dihydrogen phosphate, and yeast powder to prepare a bacterial cellulose fermentation culture solution. The pH of the culture solution is 5.0, and the bacterial cellulose fermentation culture solution is placed in In a sterilizing pot, sterilize at 120°C and 0.1MPa for 30 minutes; then, insert the activated Acetobacter xylinum species into the above-mentioned bacterial cellulose culture solution, and cultivate it on a shaking table for 12 hours; the mass volume concentration is 0.2mg / ml of graphene dispersion, ultrasonically dispersed for 1 hour, and sterilized by ultraviolet light three times, each time for 15 minutes; In the bacterial cellulose culture solution of bacterial strain; Shake the above-mentioned mixed liquid shaker for 12 hours to make the mixed liquid uniform; put the incubator at 28° C. for static cultivation for 1 week, and obtain the graphene / bacterial cellulose...

Embodiment 2

[0037] The difference between the preparation process of this embodiment 2 and the above-mentioned embodiment 1 is that, wherein, the graphene dispersion is added to the bacteria-carrying In the bacterial cellulose culture solution of species; shake the above-mentioned mixed liquid shaker for 12 hours to make the mixed liquid uniform.

[0038] figure 1 (c) and figure 1 (d) are respectively the SEM photos of the graphene / bacterial cellulose composite material of Example 2; figure 2 (b) is a TEM photo of the graphene / bacterial cellulose composite material of Example 2; FIG. 3(b) is a Raman spectrum test curve of the graphene / bacterial cellulose composite material of Example 2.

Embodiment 3

[0040]The only difference between the present embodiment 3 and the preparation process of the above-mentioned embodiment 1 is that the activated Acetobacter xylinum species is inserted into the above-mentioned bacterial cellulose culture solution, and cultured on a shaking table for 24 hours; the mass volume concentration is 0.2 mg / ml graphene dispersion, ultrasonically dispersed for 2 hours; shake the mixed liquid on a shaker for 24 hours to make the mixed liquid uniform; put it in an incubator at 28°C for static culture for 2 weeks.

[0041] figure 1 (e) and figure 1 (f) SEM photos of the graphene / bacterial cellulose composite material of Example 3; figure 2 (c) is the TEM photo of the graphene / bacterial cellulose composite material of Example 3.

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