Use method of bacteria cellulose in ancient protection of wrought silk cultural relics
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A technology for bacterial cellulose and cultural relic protection, applied in microorganism-based methods, biochemical equipment and methods, fiber processing, etc., can solve problems such as difficult operation, zero tensile strength, damage to cultural relics, etc. The effect of resistance and mechanical strength
Inactive Publication Date: 2009-07-29
荆州文物保护中心 +1
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However, the unearthed cultural relics are very fragile, and the tensile strength is close to zero. Even a slight operation may cause damag...
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preparation example Construction
[0021] The preparation method of the freeze-dried tube of Acetobacter xylinum is: take fresh skimmed milk, add its weight of 0.1% calcium carbonate, sterilize at 110°C for 15 minutes or use intermittent sterilization, sterilize at 80°C for three times, each time for 10 minutes, put wood Wash the Acetobacter cells from the inclined surface, filter them with sterile cotton, collect the cells by centrifugation, add sterile milk to suspend, draw 0.1-0.2ml of cells into a freeze-drying tube, freeze-dry at -40°C, and vacuum seal .
[0025] 1) Soak the silk cultural relics in the sterile culture medium of the fermentation medium, and rinse gently to remove the soil and other impurities attached to the surface of the cultural relics,
[0026] 2) Acetobacter xylinum is activated with a slant medium, and the preserved bacterial classification is transferred to a fresh slant medium, cultivated on a seed medium and fermented on a fermentation medium to obtain a pure culture, and the culture is combined with fresh without Bacteria culture solution was mixed in a ratio of 1:10 by weight, and packed into a micro-sprayer to prepare restoration agent a,
[0027] The seed medium cultivation method is: 500 milliliters of Erlenmeyer flasks are filled with 100 milliliters of culture medium, at 28°C, the shaker speed is 150 rpm, and cultivated for 3 days; The bed rotates at 150 rpm, cultivated for 2 days,
[0028] The slant medium is composed of 8% glucose, 1% yeast extr...
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Abstract
The invention relates to an application method of bacteria cellulose for the protection of ancient silk relics, comprising the specific steps as follows: treating the surface of the ancient silk relic to prepare two bacteria cellulose repair agents: wherein, acetobacter xylinum is activated with a slant culture medium to experience fermentation culture to obtain pure cultures which are admixed with a liquid culture medium to obtain the repair agent a, or acetobacter xylinum freeze-dried powders are dissolved with aseptic sugar-containing stroma to admix the dissolution liquid with liquid culture stroma to obtain the repair agent b; spraying an atomizing agent a or b onto the surface of the silk relic for culture to form a layer of cellulose membrane on the surface of the silk relic, and then obtain the well-processed relic after post-treatments. By making use of the characteristics of high strength and high flexibility of the bacteria cellulose generated by the acetobacter xylinum, the invention mixes and atomizes strains and the culture stroma to be dispersed on the surface of the silk relic, and a thin layer of nano-cellulose membrane is formed on the surface of the silk relic after the culture, thus improving the flexibility and mechanical strength of the ancient silk relics.
Description
technical field [0001] The invention relates to an application method of bacterial cellulose in the protection of ancient silk fabric cultural relics. Background technique [0002] Bacterial cellulose is pure cellulose synthesized by microorganisms such as Acetobacter xylinum using glucose, sucrose and other carbon sources. Its chemical structure is very similar to that of natural plant cellulose. A straight-chain polymer compound formed by glycosidic linkages. The difference is that bacterial cellulose is a pure fiber that does not contain hemicellulose and lignin. In addition, bacterial cellulose has high strength, strong water absorption, and extremely fine fibers (single fiber is as thin as nanometers) )Features. [0003] Acetobacter xylinum is a Gram-negative bacterium with a width of 0.6-0.8 μm and a length of 1.0-4.0 μm. It exists in single, paired or chain form. The colonies are round, opaque, protruding, light brown, and rough in surface , are aerobic microorgani...
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