Acetobacter xylinum and method for preparing nano-cellulose skin tissue repair material by using the same

A technology of nanocellulose and Acetobacter xylinum, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of not being able to improve the mechanical properties of materials, infection or rejection, and poor water holding capacity , to achieve high social and economic benefits, low cost, and long-term moisture retention

Inactive Publication Date: 2008-11-12
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on artificial skin has been reported successively at home and abroad, for example: 1) the application date of 2002.05.09, the application number 02117585.3, the authorization announcement date 2003.11.19, the Chinese patent application document of the patent number CN02117585.3 "A kind of artificial skin and its preparation Methods and Applications "Using type I animal collagen and autologous bone marrow mesenchymal stem cells to prepare artificial skin, but its strength is not high, and the process is complicated and the cost is high; 2) Application date 2002.01.16, application number 02100072.7, authorization announcement date 2002.08 .14, the Chinese patent application document of patent No. CN02100072.7 "multilayer artificial skin with chitin or its derivatives as the matrix grid" uses chitin porous grid as the matrix material, but its flexibility is low and cannot be better 3) Ap

Method used

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  • Acetobacter xylinum and method for preparing nano-cellulose skin tissue repair material by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of basement membrane of nanocellulose skin tissue repair materials

[0024] (1) Prepare a slant medium containing 5% glucose by mass percentage, 1% yeast powder, 2% calcium carbonate and 2% agar. The Acetobacter xylinum Y05 (Acetobacterxylinum Y05, CCTCC M207163) preserved on the slant is activated on the slant medium, and the activated bacterial classification is inoculated in a 250mL Erlenmeyer shaker flask, cultivated on a shaker as a first-class seed, and the first-class Seeds are inoculated in seed tanks, and after expanded cultivation, they are made into seed cultures for fermentation production.

[0025] (2) Prepare a liquid medium containing 10% glucose, add 1% yeast powder and 2% calcium carbonate, and its initial pH value is 5.5, and culture it statically for 7 days at a constant temperature of 30°C.

[0026] (3) Soak the cultured nanocellulose membrane in distilled water for 2 days, then put it into a NaOH solution with a mass concentration of...

Embodiment 2

[0031] 1. Preparation of basement membrane of nanocellulose skin tissue repair materials

[0032] (1) Prepare a slant medium containing 5% glucose by mass percentage, 1% yeast powder, 2% calcium carbonate and 2% agar. Acetobacter xylinum Y05 (Acetobacter xylinum Y05, CCTCC M 207163) preserved on the slant was activated on the slant medium, and the activated strain was inoculated in a 250mL Erlenmeyer shaker flask, cultivated on a shaker as a primary seed, and a The high-grade seeds are inoculated in the seed tanks, and after expanded cultivation, they are made into seed cultures for fermentation production.

[0033] (2) Prepare a liquid medium containing 12% glucose, add 1.5% yeast powder and 3% calcium carbonate, and its initial pH value is 3.5, and culture it statically at a constant temperature of 30° C. for 7 days.

[0034] 2. Combination of new nanocellulose skin tissue repair materials

[0035] Prepare a 2% collagen acetic acid solution and store at 4°C. Chitosan was ...

Embodiment 3

[0037] 1. Preparation of basement membrane of nanocellulose skin tissue repair materials

[0038] (1) Prepare slant culture medium containing 10% glucose by mass percentage, 1% yeast powder, 2% calcium carbonate and 2% agar. Acetobacter xylinum Y05 (Acetobacter xylinum Y05, CCTCC M 207163) preserved on the slant was activated on the slant medium, and the activated strain was inoculated in a 250mL Erlenmeyer shaker flask, cultivated on a shaker as a primary seed, and a The high-grade seeds are inoculated in the seed tanks, and after expanded cultivation, they are made into seed cultures for fermentation production.

[0039] (2) Prepare a liquid medium containing 15% glucose, add 2% yeast powder and 4% calcium carbonate, and its initial pH value is 6.0, and culture it statically at a constant temperature of 30° C. for 14 days.

[0040] 2. Combination of new nanocellulose skin tissue repair materials

[0041] Prepare a 2% collagen acetic acid solution and store at 4°C. Chitosa...

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Abstract

The invention provides an Acetobacter xylinum Y05 strain and a method for preparing nanometer cellulose skin tissue repairing material with the same. The preparation method comprises the following steps that: Acetobacter xylinum Y05 (CCTCC M 207163) preserved on an inclined plane is made into a shake-flask seed and cultivated statically to obtain a nanometer cellulose membrane; and the well-cultivated membrane is separated, purified and then compounded with natural polysaccharide (chitosan, etc.) and protein (collagen, silk fibroin, etc.), so as to prepare the repairing material. The nanometer repairing material has good biocompatibility, flexibility and strength which are similar to human skin, as well as good plasticity and elasticity, can be suitable for various irregular wound surfaces, can keep moisture for a long time, and can be used as a skin substitute and medical dressings for burns, chronic skin ulcers and other skin injuries or defects in clinical practice.

Description

technical field [0001] The invention relates to acetobacter xylinum strain and a method for preparing nano bacterial cellulose film through it. technical background [0002] There are about 15 million patients with burns and ulcers every year in my country, and nearly 10 million of them need skin transplantation treatment. The total market capacity is 3.88×10 4 m 2 . The huge market potential provides a broad market prospect for the industrialization of skin tissue engineering. [0003] For cases of large-area skin loss such as II / III degree burns and refractory skin ulcers, clinical treatment is very difficult due to the large area of ​​skin lesions and severe damage to the subcutaneous tissue structure, and it is often difficult for patients to survive shock and infection. Early use of human skin to seal wounds, reduce body fluid loss, and prevent pathogenic microorganisms from invading can save patients' lives and help wounds heal, while providing support and microenvi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A61L27/24A61L27/22A61L27/20A61L27/60C12R1/02
Inventor 杨光付丽娜何峰周平余龙江
Owner HUAZHONG UNIV OF SCI & TECH
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