Acetobacter xylinum and method for preparing nano-cellulose skin tissue repair material by using the same

Abstract

The invention provides an Acetobacter xylinum Y05 strain and a method for preparing nanometer cellulose skin tissue repairing material with the same. The preparation method comprises the following steps that: Acetobacter xylinum Y05 (CCTCC M 207163) preserved on an inclined plane is made into a shake-flask seed and cultivated statically to obtain a nanometer cellulose membrane; and the well-cultivated membrane is separated, purified and then compounded with natural polysaccharide (chitosan, etc.) and protein (collagen, silk fibroin, etc.), so as to prepare the repairing material. The nanometer repairing material has good biocompatibility, flexibility and strength which are similar to human skin, as well as good plasticity and elasticity, can be suitable for various irregular wound surfaces, can keep moisture for a long time, and can be used as a skin substitute and medical dressings for burns, chronic skin ulcers and other skin injuries or defects in clinical practice.

Description

technical field [0001] The invention relates to acetobacter xylinum strain and a method for preparing nano bacterial cellulose film through it. technical background [0002] There are about 15 million patients with burns and ulcers every year in my country, and nearly 10 million of them need skin transplantation treatment. The total market capacity is 3.88×10 4 m 2 . The huge market potential provides a broad market prospect for the industrialization of skin tissue engineering. [0003] For cases of large-area skin loss such as II / III degree burns and refractory skin ulcers, clinical treatment is very difficult due to the large area of ​​skin lesions and severe damage to the subcutaneous tissue structure, and it is often difficult for patients to survive shock and infection. Early use of human skin to seal wounds, reduce body fluid loss, and prevent pathogenic microorganisms from invading can save patients' lives and help wounds heal, while providing support and microenvi...

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Problems solved by technology

[0006] At present, the research on artificial skin has been reported successively at home and abroad, for example: 1) the application date of 2002.05.09, the application number 02117585.3, the authorization announcement date 2003.11.19, the Chinese patent application document of the patent number CN02117585.3 "A kind of artificial skin and its preparation Methods and Applications "Using type I animal collagen and autologous bone marrow mesenchymal stem cells to prepare artificial skin, but its strength is not high, and the process is complicated and the cost is high; 2) Application date 2002.01.16, application number 02100072.7, authorization announcement date 2002.08 .14, the Chinese patent application document of patent No. CN02100072.7 "multilayer artificial skin with chitin or its derivatives as the matrix grid" uses chitin porous grid as the matrix material, but its flexibility is low and cannot be better 3) Application date 2002.12.04, application number 02151020.2, authorization announcement date 2004.06.16, patent number CN02151020.2 Chinese patent application document "Tissue engineering using xenogeneic or allogeneic bone matrix gelatin as scaffold "Artificial skin" uses heterogeneous or heterogeneous bone matrix gelatin as a scaffold to prepare artificial skin with tissue structure characteristics similar to normal human skin, but materials from heterogeneous or heterogeneous sources may cause infection or rejection; 4) Application date 2004.12.28, application No. 200410081608.8, authorized announcement date 2006.07.05, patent No. CN200410081608.8 Chinese patent application document "a nano-chitosan artificial skin and its manufacturing method" The nano-chitosan artificial skin obtained has a three-dimensional network structure, but its The water is poor and cannot provide a long-term moist environment for the wound;

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