Preparation method of bacterial cellulose

A technology of bacterial cellulose and cellulose, which is applied in the field of industrial biology, can solve problems such as immaturity, and achieve the effects of reducing fermentation costs, simple process, and low cost

Active Publication Date: 2017-02-15
南京生命原健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some achievements have been made in research, it is still not mature enough to transform research into production.

Method used

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  • Preparation method of bacterial cellulose
  • Preparation method of bacterial cellulose

Examples

Experimental program
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Embodiment 1

[0030] Embodiment 1: 7L NBS tank fermentation

[0031] 1) Shake flask strain preparation: scrape a ring of slanted seeds and connect them to the sterilized seed medium (glucose 20, yeast extract 2, peptone 2, CaCO3 0.5, unit g / L, pH value natural), 28°C, 100r / min cultured for 16 hours and then expanded with culture;

[0032] 2) Preparation of molasses: pre-dilute the original sugarcane molasses by 2 times, adjust the pH value to 2.0 with sulfuric acid, hydrolyze at 90° C. for 30 minutes, centrifuge or filter the supernatant after standing overnight and then dilute 10 times, the pH value is 6.0;

[0033] 3) Fermentation: inoculate the seeds in a certain amount of sugarcane molasses medium (molasses 25, glycerol 0.5, corn steep liquor 10g / L, ammonium sulfate 0.5, citric acid 1.0, disodium hydrogen phosphate 2.0, magnesium sulfate 0.2, Unit g / L) fermenter was stirred and fermented for 8 days; wherein, the stirred tank was a bioreactor equipped with gauze, and the gauze was hung ...

Embodiment 2

[0035] Example 2: 50 liters of stirred tank fermentation

[0036] 1) Strain preparation: Scrape a ring of slanted seeds and connect them to the sterilized seed medium (glucose 40, yeast extract 5, peptone 5, CaCO3 1, unit g / L, pH value natural), 32°C, 150r / After 24 hours of culture, move to the NBS tank in Example 1 for expansion, the liquid volume is 3L, the culture medium is similar to the shake flask, the rotation speed is 100r / min, the ventilation rate is 1.0vvm, and the temperature is 32°C and the culture is stirred for 20 hours;

[0037] 2) Preparation of molasses: pre-dilute the beet molasses by 5 times, adjust the pH value to 2.5 with sulfuric acid, hydrolyze for 20 minutes at 100° C., centrifuge or filter the supernatant after standing overnight and then dilute it by 2 times, and the pH value is 5.5;

[0038]3) Fermentation: Inoculate the seeds in 30L molasses medium (molasses 50, glycerin 0.2, corn steep liquor 20g / L, ammonium sulfate 1.5, citric acid 1.5, disodium ...

Embodiment 3

[0040] Embodiment 3: 2 tons of stirred tank fermentation

[0041] 1) Strain preparation: Scrape a ring of slanted seeds and connect to 100mL seed medium (glucose 25, yeast extract 2.5, peptone 2.5, CaCO 3 3, unit g / L, pH value natural) 500mL Erlenmeyer flask, cultured at 30°C, 150r / min for 20 hours, then cultured in a 5L Erlenmeyer flask with 1L medium for 20 hours under the same conditions. Move to a 200L seed tank with 100L medium for expansion. The medium is similar to that of a shaker flask, and the rotation speed is 90r / min, the ventilation rate is 1.5vvm, and the temperature is 30°C and stirred for 24 hours;

[0042] 2) Preparation of molasses: pre-dilute sugarcane molasses 4 times, adjust the pH value to 3.0 with sulfuric acid, hydrolyze at 120° C. for 15 minutes, centrifuge or filter the supernatant after standing overnight and then dilute 2.5 times, the pH value is 4.8;

[0043] 3) Fermentation: move the seeds to inoculate in 1200L molasses medium (molasses 50, glyc...

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Abstract

The invention discloses a preparation method of bacterial cellulose. The preparation method comprises the following steps that (1) preparation of shake flask bacteria: acetobacter xylinum ATCC 700178 is inoculated to a seed culture medium and cultured at the temperature of 28 DEG C to 33 DEG C for 12-24 h, and a first-stage seed solution is obtained; (2) enlarge cultivation of the seed: the first-stage seed solution is inoculated to the seed culture medium and subjected to enlarge cultivation, and a second-stage solution is obtained; (3) fermentation: the second-stage seed solution is inoculated to a fermentation tank filled with a molasses culture medium, the fermentation tank is filled with immobilized media, and fermentation is performed for 6-8 days; (4) extraction: the biological nano-crystalline cellulose is extracted from the fermentation liquor. According to the preparation method of the bacterial cellulose, the yield of the biological nano-crystalline cellulose can be improved greatly, the obtained biological nano-crystalline cellulose product is cotton-shaped or flake-shaped, the fibrous structure is looser, and the degree of polymerization is smaller.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a method for efficiently fermenting and producing biological nanocellulose. Background technique [0002] Compared with plant cellulose, bionanocellulose has many unique properties, such as high water holding capacity, high crystallinity, high biocompatibility, ultra-fine network structure, high tensile strength and elastic modulus, etc., due to These characteristics make it widely used in food, biomedicine, acoustic equipment, papermaking, fuel cells, ion exchange membranes and membrane separation and other fields. For example, in the field of medical materials, it can be made into wound accessories such as artificial skin, gauze, bandages and "band-aids". [0003] At present, there are two types of bionanocellulose fermentation methods: dynamic and static. Different fermentation methods lead to poor polymerization degree and large differences in structur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 陈勇应汉杰刘庆国赵楠刘桂文郭亭
Owner 南京生命原健康科技有限公司
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