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Method for preparing chitosan oligosaccharide/bacterial cellulose sponge

A technology of bacterial cellulose and chitosan oligosaccharides, which is applied in medical science, absorbent pads, bandages, etc., can solve the problems of complex process, water absorption and coagulation effect not meeting the requirements, and achieve simple preparation process, water absorption and permeability. Good wetness and good coagulation effect

Inactive Publication Date: 2013-04-03
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the process is complicated, and the water absorption and coagulation effect cannot meet the requirements

Method used

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  • Method for preparing chitosan oligosaccharide/bacterial cellulose sponge
  • Method for preparing chitosan oligosaccharide/bacterial cellulose sponge
  • Method for preparing chitosan oligosaccharide/bacterial cellulose sponge

Examples

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Effect test

example 1

[0027] Example 1: Take the Acetobacter xylinum cell culture solution, centrifuge at 8000 g, discard the supernatant, wash the pellet with PBS, centrifuge and discard the supernatant, add 40 μl of 0.5 mg / ml MTT, incubate at 30°C for 1 hour, 4 parallel samples in each group, Measure OD at 570nm using a microplate reader 570 Value, average value; The measured OD value is 0.2191.

example 2

[0028] Example 2: Take the Acetobacter xylinum cell culture solution, centrifuge at 8000 g, discard the supernatant, wash the precipitate with PBS, discard the supernatant by centrifugation, add 40 μl of 0.5 mg / ml MTT, incubate at 30°C for 2 hours, and each group has 4 parallel samples, Measure OD at 570nm using a microplate reader 570 Value, average value; The measured OD value is 0.2412.

example 3

[0029] Example 3: Take the Acetobacter xylinum cell culture solution, centrifuge at 8000 g, discard the supernatant, wash the precipitate with PBS, discard the supernatant by centrifugation, add 40 μl of 0.5 mg / ml MTT, incubate at 30°C for 3 hours, and each group has 4 parallel samples, Measure OD at 570nm using a microplate reader 570 Value, average value; The measured OD value is 0.3188.

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Abstract

The invention discloses a method for preparing chitosan oligosaccharide / bacterial cellulose sponge. The method comprises the steps of: screening out acetobacter xylinum at the stage with highest activity by means of MTT (Methyl Thiazolyl Tetrazolium Colourimetry); adding the chitosan oligosaccharide into a culture system to prepare a chitosan oligosaccharide / bacterial cellulose compound; rinsing the chitosan oligosaccharide / bacterial cellulose compound using distilled water; adding 1% sodium hydroxide solution into the rinsed compound to boil for 4 hours; soaking the compound at room temperature for 0.5 hour by adding 0.5% acetic acid; flushing the compound 5 times using distilled water; and freezing and drying the above processed compound to prepare the chitosan oligosaccharide / bacterial cellulose sponge. The chitosan oligosaccharide / bacterial cellulose sponge prepared by the invention is in a three-dimensional network structure, high in porosity, and dense and uniform in interspace. Experiments prove that the chitosan oligosaccharide / bacterial cellulose sponge is better in hydroscopicity, moisture permeability and blood coagulation effect in comparison with a traditional wound dressing; cytotoxicity by biocompatibility test is 0-grade; the raw materials are easily available; and the preparation process is simple. The chitosan oligosaccharide / bacterial cellulose sponge prepared by the invention is easy to achieve industrialized production and is good in biocompatibility, and thus can be widely used in the field of biomedical materials.

Description

technical field [0001] The invention relates to a method in the technical field of wound dressings, in particular to adding chitosan to the culture medium of Acetobacter xylinum to prepare chitosan with better water absorption and moisture permeability, better coagulation effect, and biocompatible chitosan Sugar / bacterial cellulose sponge method. Background technique [0002] In the field of biomedicine, due to its unique biocompatibility and biocompatibility as well as high water holding capacity and crystallinity, bacterial cellulose has been studied more and more in artificial blood vessels, medical dressings and tissue engineering scaffolds. The cellulose with the best performance in the world has become a research hotspot of new biomedical materials in the world in recent years. [0003] Oligochitosan has good water solubility and is easily absorbed by the human body, which makes oligochitosan exhibit more unique functional properties and physiological activities. Oli...

Claims

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Application Information

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IPC IPC(8): A61L15/28
Inventor 曹献英陈胜杰操凤郑育声杜杰陈欢
Owner HAINAN UNIVERSITY
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